| Literature DB >> 25462452 |
Maryline Olivier, Remain BottG, Eric Frisdal, Marion Nowick, Wanee Plengpanich, Charles Desmarchelier, Stéphanie Roi, Carmel M Quinn, Ingrid Gelissen, Wendy Jessup, Miranda Van Eck, Maryse Guérin, Wilfried Le Goff, Emmanuelle Reboul.
Abstract
Vitamin E membrane transport has been shown to involve the cholesterol transporters SR-BI, ABCA1 and NPC1L1. Our aim was to investigate the possible participation of another cholesterol transporter in cellular vitamin E efflux: ABCG1. In Abcgl-deficient mice, vitamin E concentration was reduced in plasma lipoproteins whereas most tissues displayed a higher vitamin E content compared to wild-type mice. α- and γ-tocopherol efflux was increased in CHO cells overexpressing human ABCG1 compared to control cells. Conversely, α- and γ- tocopherol efflux was decreased in ABCG1-knockdown human cells (Hep3B hepatocytes and THP-1 macro- phages). Interestingly, α- and γ-tocopherol significantly downregulated ABCG1 and ABCA1 expression levels in Hep3B and THP-1, an effect confirmed in vivo in rats given vitamin E for 5 days. This was likely due to reduced LXR activation by oxysterols, as Hep3B cells and rat liver treated with vitamin E displayed a significantly reduced content in oxysterols compared to their respective controls. Overall, the present study reveals for the first time that ABCG1 is involved in cellular vitamin E efflux.Entities:
Mesh：
Substances：
Year: 2014 PMID： 25462452 DOI： 10.1016/j.bbalip.2014.10.003
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002