| Literature DB >> 25461909 |
Suqin Qi1, Wenjuan Fu2, Chengmin Wang2, Changjiang Liu2, Chao Quan2, Ansoumane Kourouma2, Maosheng Yan2, Tingting Yu2, Peng Duan2, Kedi Yang3.
Abstract
Bisphenol-A was examined for its effects on cultured Sertoli cells established from 18 to 22-day-old rat testes. Results indicated that exposure to BPA (0, 30, 50 and 70 μM) decreased the cell viability in a concentration-dependent manner and induced cell apoptosis. Apoptosis-caused cell death was observed in cells exposed to 50 and 70 μM BPA. The mRNA expressions of Fas, FasL and caspase-3 were all elevated, and the protein expressions of FasL and cleaved caspase-3 were also increased. In addition, levels of phosphorylation of JNKs/p38 MAPK were also increased and then activated JNKs/p38 MAPK up regulated target gene expressions, such as c-jun and CHOP. Translocation of NF-κB into nuclei indicated the activation of NF-κB after treatment with BPA. Taken together, observed results suggest that BPA induces apoptosis of Sertoli cells by the activation of JNKs/p38 MPAK and translocation of NF-κB, and Fas/FasL system plays a critical role in the initiation of apoptosis.Entities:
Keywords: Bisphenol-A; Cells apoptosis; Fas/FasL; MAPK; NF-κB
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Year: 2014 PMID： 25461909 DOI： 10.1016/j.reprotox.2014.10.013
Source DB: PubMed Journal: Reprod Toxicol ISSN： 0890-6238 Impact factor: 3.143