Combination of letrozole, metronomic cyclophosphamide and sorafenib is well-tolerated and shows activity in patients with primary breast cancer.

PURPOSE: To assess whether the combination of letrozole, metronomic cyclophosphamide and sorafenib (LCS) is well tolerated and shows activity in primary breast cancer (BC).
METHODS: Thirteen oestrogen receptor-positive, postmenopausal, T2-4, N0-1 BC patients received the LCS combination for 6 months. In these patients we examined the pharmacokinetics of sorafenib and cyclophosphamide, toxicity of the regimen, the clinical response to therapy and changes in the levels of biologically relevant biomarkers.
RESULTS: Adequate plasma concentrations of sorafenib were achieved in patients when it was dosed in combination with L+C. The mean plasma concentrations of C were consistently lower following administration of LCS, compared with administration of L+C only. The most common drug-related grade 3/4 adverse events were skin rash (69.3%), hand-foot skin reaction (69.3%) and diarrhoea (46.1%). According to RECIST Criteria, a clinical complete response was observed in 6 of 13 patients. A significant reduction in tumour size, evaluated with MRI, was also observed between baseline and 14 days of treatment in all 13 patients (P=0.005). A significant reduction in SUV uptake, measured by (18)FDG-PET/CT, was observed in all patients between baseline and 30 days of treatment (P=0.015) and between baseline and definitive surgery (P=0.0002). Using modified CT Criteria, a response was demonstrated in 8 out of 10 evaluable patients at 30 days and in 11 out of 13 evaluable patients at the definitive surgery. A significant reduction in Ki67 expression was observed in all patients at day 14 compared with baseline (P<0.00001) and in 9 out of 13 patients at the definitive surgery compared with baseline (P<0.03). There was also a significant suppression of CD31 and VEGF-A expression in response to treatment (P=0.01 and P=0.007, respectively).
CONCLUSIONS: The LCS combination is feasible and tolerable. The tumour response and target biomarker modulation indicate that the combination is clinically and biologically active.

Treatment with aromatase inhibitors (AIs), such as letrozole, has demonstrated increased response rate compared with tamoxifen in patients with breast cancer (BC) (Smith and Dowsett, 2003). However, the development of resistance to endocrine therapy is a significant problem involving the activation of other signalling pathways such as EGFR, HER2, IGFR, MAPK, PI3K-AKT or PKC (Nicholson , 2005; Osborne ; Generali ). This provides a strong rationale for combining endocrine therapies with signal-transduction inhibitors to bypass endocrine therapy resistance and achieve a more potent anti-tumour effect (Gligorov ; Johnston ).

Sorafenib (Nexavar, BAY43-9006) is an oral multi-targeted kinase inhibitor, which is capable of inhibiting several kinases involved in tumour progression and angiogenesis (Iyer ). It was also recently shown that sorafenib can inhibit both mTOR and (Kumar ; Huynh ) and MAPK signalling (Keswani ). Moreover, our preclinical data demonstrate that the combination of letrozole with sorafenib in aromatase-expressing BC cells shows a synergistic inhibition of cell proliferation in vitro (Bonelli ). We showed that sorafenib inhibited BC-cell proliferation in a concentration-dependent manner through a mechanism involving downregulation of mTORC and its downstream targets p70S6K and 4E-binding protein 1 (4E-BP1) (Bonelli ). In BC cells treated with letrozole and sorafenib, suppression of cell proliferation was associated with enhanced accumulation of cells in the G0/G1 phase of the cell cycle due to a downregulation of c-myc, cyclin D1 and phospho-Rb expression (Bonelli ). Moreover, experiments involving long-term exposure to sorafenib showed that it prevented the acquisition of resistance towards letrozole in BC cells in vitro (Bonelli ). These preclinical data provide a strong rational for testing letrozole combined with sorafenib in patients with BC.

Metronomic chemotherapy is the frequent administration of cytotoxic drugs at a low dose that is designed to avoid dose-limiting adverse effects that would require rest periods. In addition, metronomic chemotherapy is proposed to target tumour growth indirectly by inhibiting angiogenesis, rather than directly targeting tumour cells (Pasquier ). Our group has previously shown that the combination of letrozole with a metronomic regimen of cyclophosphamide is active in elderly patients with primary BC (Bottini ). These data do contrast with studies that combined endocrine therapy with conventional high-dose chemotherapy regimens, where the combination was shown to be antagonistic (Osborne ; Albain ). However, the favourable results we reported with metronomic cyclophosphamide probably stem from the lower-toxicity profile observed with this regimen and the fact that this therapy targets the vascular compartment instead of targeting tumour cells directly.

On the basis of these findings, we have planned a randomised phase II trial to explore the activity of the letrozole, metronomic chemotherapy and sorafenib combination. This is a neoadjuvant trial in postmenopausal breast cancer patients with ER positive, HER2 negative primary breast cancer and is currently ongoing (Eudract Number 2007-006208-39). In this paper, we present pharmacokinetic, toxicity, response and biomarker data for this regimen in the first thirteen consecutive patients enrolled in the study before randomisation.

Materials and methods

Patients and treatment

This study involved 13 postmenopausal women with ER positive, HER2 negative, T2-4, N0-1 BC enrolled in a single institution. Inflammatory breast cancer was excluded from the study. Eligibility criteria for inclusion were the same as those described for our previous study, which examined the combination of letrozole with metronomic cyclophosphamide in BC (Bottini ). Patients received one tablet (2.5 mg) of letrozole per day (Femara; Novartis), one tablet (50 mg) of cyclophosphamide per day (Endoxan; Baxter) and every 5th day they received two tablets (400 mg bid) of sorafenib (Nexavar, Bayer). These drugs were given continuously for 6 months. At the end of treatment the patients underwent definitive surgery. All adverse events (AE) were graded by the National Cancer Institute Common Toxicity Criteria (NCI-CTC), version 3.0.

With regard to the pharmacokinetic profile, during the first 5 days the patients received only letrozole and cyclophosphamide. The administration of sorafenib started from day 6 onwards. The pharmacokinetic profile of sorafenib was assessed on day 29. The pharmacokinetic profile of cyclophosphamide and 4-hydroxy-cyclophosphamide was assessed on days 5 and 29. This approach permitted us to test the safety and tolerability of this regimen and the pharmacokinetic interaction between sorafenib and cyclophosphamide. The decision to continue the study or not depended on evaluations of tolerability, safety and pharmacokinetic profile of cyclophosphamide after 6 weeks. A dose escalation was not planned. If <33% of these patients experienced no significant toxicity on the treatment, the study continued until the conclusion of the pre-planned enrolment. If 33% or more of the patients experienced a significant toxicity during the treatment, the study would have been closed due to unacceptable toxicity. The dose-limiting unacceptable toxicity was defined by the following adverse events during the first cycle that could be correlated to the drug combination:

Haematological toxicity:

Neutropenia <0.5 × 109 l−1 not associated with fever lasting longer than 10 days

Neutropenia <0.5 × 109 l−1 associated to fever >38.5 °C

Piastrinopenia <25 × 109 l−1 or grade 3 piastrinopenia associated with bleeding

Non-haematological toxicity:

grade 3–4 non-haematological toxicity with the exclusion of:

allergic reactions controlled by an adequate therapy

grade 4 hypertension crises related to sorafenib

grade 3 hypertension

Any toxicity correlated to the study drug was unacceptable according to the opinion of the investigators.

Pharmacokinetic analysis

The pharmacokinetic analysis was performed in the Laboratory of Dr Frank-Thorsten Hafner (Bayer HealthCare AG, Preclinical Pharmacokinetics—Bioanalytics, Pharma Research Center, Building 468 D-42096 Wuppertal, Germany). For measurement of sorafenib, plasma samples were collected on day 1 of Cycle 2 (Day 29) following multiple dosing of both 400 mg bid sorafenib and 50 mg of cyclophosphamide in combination with 2.5 mg of letrozole (see Figure 1A). Plasma samples from 13 patients were collected before dosing and at 0.5, 1, 2, 4, 8, 10 and 12 h after dosing. For measurement of cyclophosphamide and its metabolite 4-hydroxy-cyclophosphamide, plasma samples were collected on Day 5 of Cycle 1 (before sorafenib treatment) and on Day 1 of Cycle 2 (Day 29) (after concomitant treatment with multiple oral doses of both drugs) (see Figure 1A). This allowed us to evaluate the effect of multiple oral doses of sorafenib on the pharmacokinetics of cyclophosphamide. Plasma samples from 13 patients were collected before dosing and at 0.25, 0.5, 0.75, 1, 1.5, 2, 3, 4, 6, 8, 10, 12 and 24 h after dosing. The concentration of the drugs was assessed by using a one-way analysis of variance model for dose-normalised and log-transformed maximum observed serum concentration considering the concentration-time curve (AUC). Statistical significance of the factor of dose was set at P=0.05.

Figure 1

(A) Schematics illustrating the design of the study. (B) Pharmacodynamic profile of the study.

Response assessment

The size of the primary tumour and the axillary lymph nodes was measured monthly with callipers by the same clinician. Tumour size measurements obtained by breast MRI, using protocols described elsewhere (Bonelli ), were made at baseline and at the 14th day of treatment and were independently reviewed by two experienced radiologists. Any discrepant readings were resolved by consensus. Response evaluated by either clinical palpation or breast MRI was assessed by the measurement of the changes in the product of the two largest diameters recorded at baseline, at the 14th day and at the end of therapy before surgery, according to the RECIST Criteria.

The 18FDG-PET/CT scans (CT 8 slice) were carried out using a Discovery ST PET/CT scanner (GE Healthcare, Milwaukee, WI, USA) and performed at baseline, after 30 days of treatment and before definitive surgery. The 18FDG-PET/CT images were read independently by two experienced radiologists. Standardised uptake values (SUVs) were calculated. Lesions were scored for response by Modified CT Criteria using 18FDG-PET/CT scans obtained at baseline, after 30 days of treatment and just before definitive surgery. Lesions were scored as follows: CR (disappearance of lesion), PR (decrease in tumour size of ⩾10% or decrease in tumour density of ⩾15%), SD (does not meet the criteria for CR, PR or PD) and PD (an increase in tumour size of ⩾10% and does not meet criteria of PR by tumour density on CT) (Choi ).

The observers were blinded for any clinical data including the site of the primary tumour. Discrepancies were resolved by consensus. During the study, clinicians and pathologists were blinded to the 18FDG-PET/CT related data.

Collection and processing of tissue samples

Tissue was obtained from patients at three time points: tissue from incision biopsy performed at presentation, tissue from tru-cut biopsy performed after 14 days of treatment and tissue obtained at definitive surgery. Surgery (quadrantectomy or mastectomy) was planned after full clinical reassessment. All patients subjected to quadrantectomy underwent irradiation of the residual breast (60 Gys delivered in 6 weeks). Immunohistochemistry was performed on paraffin-embedded tumour samples obtained at all three time points. HER-2, ER, PgR, Ki67, CD31 and VEGF-A staining was performed using standard protocols as described elsewhere (Koukourakis ; Bottini ).

Statistical analysis

Ki67, CD31, VEGF-A, MRI and SUVmax were analysed as continuous variables. Non-parametric statistical methods (Mann–Whitney test for unpaired data, Wilcoxon's matched-pairs signed-rank test for paired data, Spearman Rho for simple correlation analysis) were used in the primary analyses of the data. The PK analysis was carried out using ANOVA test. All tests were two-sided; P<0.05 was considered as statistically significant. Statistical analysis was performed on an IBM-compatible personal computer using Statistica software (Statsoft, Tulsa, OK, USA) for Windows (Microsoft, Redmond, WA, USA) software.

Ethical approval

The Local Ethical Committee of A.O. Istituti Ospitalieri di Cremona approved this prospective study and signed informed consent was obtained from each patient.

Results

Details of the study population

Thirteen postmenopausal women (median 67 years old, range 53–79) with T2-4, N0-1 (11 patients with T2, and 2 with T4, 6 with node negative and 7 with node positive, 5 with grade 2 and 8 with grade 3) and ER+ve (13/13) and HER2-ve (0/13) BC were prospectively enrolled between April 2009 and May 2010 into the trial. See Table 1A and 1B for details of patient characteristics.

Table 1A

Preliminary pharmacokinetic parameters of sorafenib following multiple doses of 400 mg per b.i.d. sorafenib in combination with 50 mg of cyclophosphamide and 2.5 mg of letrozole on Day 1 of Cycle 2

Patient No.	AUC(0–12) (mg h l−1)	Cmax (mg l−1)
1	80.2	8.40
2	101	10.7
3	263	23.2
5	45.7	4.98
6	56.0	5.73
7	86.8	11.4
8	103	11.6
9	46.3	6.10
10	79.0	9.00
11	113	16.4
12	41.7	4.22
13	94.5	17.6
Geometric mean	82.7	9.77
Geometric s.d.	1.64	1.68

Table 1B

Preliminary pharmacokinetic parameters of cyclophosphamide following multiple doses of 50 mg od cyclophosphamide in combination of 2.5 mg of letrozole without (Day 5, Cycle 1) and after concomitant treatment (Day 1, Cycle 2) with multiple oral doses of 400 mg bid sorafenib

	AUC(0–24) (mg h l−1)			Cmax (mg l−1)			t1/2 (h)
Patient No.	Cycle 1	Cycle 2	Ratio cycles 2/1	Cycle 1	Cycle 2	Ratio cycles 2/1	Cycle 1	Cycle 2
1	22.4	21.4	0.96	1.86	1.89	1.02	13.7	9.86
2	20.1	12.1	0.60	2.28	1.61	0.71	7.56	4.74
3	19.7	10.7	0.54	1.91	1.13	0.59	8.13	7.41
5	6.58	5.51	0.84	1.57	1.43	0.91	3.25	3.10
6	19.8	14.4	0.73	2.60	2.29	0.88	8.46	6.83
7	12.0	7.99	0.67	1.56	1.44	0.92	6.16	4.52
8	16.6	12.9	0.78	2.19	2.67	1.22	7.25	5.04
9	21.1	17.8	0.84	2.20	2.12	0.96	8.76	7.15
10	19.3	17.1	0.89	1.98	1.80	0.91	9.27	7.29
11	35.2	21.2	0.60	2.74	1.99	0.73	13.8	8.88
12	14.9	10.2	0.68	1.72	1.48	0.86	5.81	3.69
13	11.7	8.17	0.70	2.07	1.90	0.92	5.84	3.92
Meana	16.7	11.9	0.71	2.01	1.77	0.88	7.39	5.43
S.d.b	1.50	1.52	1.20	1.19	1.26	1.20	1.48	1.47

Geometric means.

Geometric s.d.

Pharmacokinetic analysis of sorafenib

We examined whether the pharmacokinetics of sorafenib were affected when sorafenib was dosed in combination with letrozole and metronomic cyclophosphamide. Plasma samples were collected on day 1 of Cycle 2 (Day 29) at which time point patients had already received multiple doses of sorafenib, cyclophosphamide and letrozole (Figure 1A). Plasma samples from 13 patients were collected before dosing and at 0.5, 1, 2, 4, 8, 10 and 12 h after dosing. A time course of circulating sorafenib concentrations is shown in Figure 2A. The results for AUC (0–12) and Cmax of sorafenib for Day 1 of Cycle 2 are given as individual and geometric mean values (see Table 1A). The geometric means for AUC (0–12) and Cmax calculated were within the range of values observed after multiple dosing with 400 mg per b.i.d. sorafenib in previous studies (Strumberg , 2007). These findings show that circulating concentrations of sorafenib were not significantly affected by dosing in combination with cyclophosphamide and letrozole.

Figure 2

Pharmaockinetics of sorafenib and cyclophosphamide. (A) Plasma concentrations of sorafenib following multiple doses of 400 mg per b.i.d. sorafenib in combination with 50 mg of cyclophosphamide and 2.5 mg of letrozole on Day 1 of Cycle 2 (geometric means, geometric s.d.; n=13). (B) Plasma concentrations of cyclophosphamide (*) following multiple doses of 50 mg of cyclophosphamide in combination with 2.5 mg of letrozole without (Day 5, Cycle 1) and after concomitant treatment (Day 1, Cycle 2) with multiple oral doses of 400 mg per b.i.d. sorafenib (G.M.=geometric means, G.s.d.= geometric standard deviation; n=13) *More than one-third of individual plasma concentrations were below LLOQ at 24 h after dosing on both profile days. No corresponding geometric mean concentrations was calculated.

Pharmacokinetic analysis of cyclophosphamide

We then examined whether dosing of sorafenib affects the pharmacokinetics of cyclophosphamide. Plasma samples were collected on Day 5 of Cycle 1 (before sorafenib treatment) and on Day 1 of Cycle 2 (Day 29) (after concomitant treatment with multiple oral doses of both sorafenib and cyclophosphamide) (see Figure 1A). This allowed us to evaluate the effect of multiple oral doses of sorafenib on the pharmacokinetics of cyclophosphamide. Although cyclophosphamide was measurable in all samples up to 12 h, only a few isolated plasma samples obtained at 24 h contained concentrations above the lower limit of quantification (LLOQ), so we limited our pharmacokinetic analysis to the time points up to 12 h. Geometric mean plasma concentration time courses of cyclophosphamide obtained in patients before and after exposure to sorafenib are shown in Figure 2A. The results for AUC (0–24), Cmax and t1/2 of cyclophosphamide for Day 5 of Cycle 1 and for Day 1 of Cycle 2 are given as individual and geometric mean values in Table 1B. Mean plasma concentrations of cyclophosphamide were consistently lower following concomitant administrations of sorafenib and letrozole compared with concomitant administration of letrozole alone (Figure 2B). This trend was confirmed by the comparison of corresponding AUC (0–24) and Cmax values from Day 5, Cycle 1 and Day 1 of Cycle 2. AUC (0–24) of cyclophosphamide was consistently lower in all subjects when both drugs were co-administered, on average by 29% (Table 1B). Cmax was also reduced in most subjects on Day 1 of Cycle 2 but to a lesser degree, on average by 12%. The decrease in AUC (0–24) was accompanied by a corresponding decrease in t1/2, on average by 27%. These data indicate that concomitant administration of sorafenib moderately decreases the exposure to cyclophosphamide.

Toxicity assessment

We did not observe particular haematological toxicity; only neutropenia grade 1–2 (38.5% of the cases) and grade 3 (7.7% of the cases). The non-haematological toxicity of letrozole and cyclophospamide in combination with sorafenib was mainly grade 1 or 2 as reported in Table 2. The most common drug-related grade 3–4 adverse events were skin rash (69.3%), hand-foot skin reaction (69.3%), diarrhoea (46.1%). Examples of skin rash and hand-foot skin reaction are depicted in Figure 3A and B, respectively. Dosing interruptions or reductions due to an AE did not occur.

Table 2

Adverse Events

Adverse Events	Grade 1 or 2	Grade 3 or 4
Hand-foot syndrome	4 (30.7%)	9 (69,3%)
Fatigue	4 (30.7%)	1 (7.7%)
Rash	4 (30.7%)	9 (69.3%)
Emesis	0	0
Hypertension	2 (15.4%)	0
Nausea	0	0
Arthralgias	3 (23%)	0
Diarrhea	2 (15.4%)	6 (46.1%)
Dehydration	4 (30.7%)	2 (23%)
Infection	0	0
Anorexia	4 (30.7%)	0
Headache	1 (7.7%)	0
Mucositis	2 (15.4%)	0
Elevated liver function tests	0	0
Rigors/chills	1 (7.7%)	0
Joint function	3 (23%)	1 (7.7%)
Neutropenia	5 (38.5%)	1 (7.7%)
Dyspnea	0	0
Acne	3 (23%)	1 (7.7%)
Hypotension	0	0
Hypophosphatemia	0	0
Alopecia	10 (76.9%)	0
Sensory neuropathy	5 (38.5%)	0
Weight loss	5 (38.5%)	0

Figure 3

Common drug-related adverse events. (A) Grade 2 skin rash. (B) Grade 2 hand-foot syndrome. Both of these reactions are adverse events related to the treatment with sorafenib.

Response assessment by palpation, mammography and pathology

Complete response by clinical palpation, evaluated at the end of treatment before definitive surgery, was observed in 6 out of 13 patients (46%), whereas partial response was observed in the remaining 7 patients (54%). Complete response by mammography was observed in 5 out of 13 patients (38.4%), whereas partial response was observed in the remaining 8 patients (61.6%) before definitive surgery. At the definitive surgery, we observed two patients with pT1b tumour, three patients with pT1c tumour, five patients with pT2 tumour, two patients with pT4b tumour, with 11 out of 13 patients having lymph node involvement. No change of tumour grading between the baseline sample and post-treatment samples was noted. None of the enrolled patients showed a post-treatment pathologic complete response.

Imaging assessment by MRI, FDG-PET and Modified CT criteria

Tumour response was assessed by an MRI scan at baseline and at 14 days of treatment (Figure 1B). Median tumour size evaluated by an MRI was 31 mm (range 17–90 mm) at baseline, 27 mm (range 11–90 mm) at day 14 with a small but statistically significant reduction in tumour size being observed in all 13 patients at day 14 (P=0.005).

The metabolic activity of the tumours was evaluated by a 18FDG -PET/CT scan at baseline (PET1), after 1 month of treatment (PET2) and at the end of the treatment before definitive surgery (PET3) for all 13 patients (Figures 1B, 4A and 4B). At PET1 the mean average SUVmax was 5.01 with a median of 4.5 (range 1.8–12). At PET 2 the mean average SUVmax was 2.39 with a median of 1.9 (range 0–4.5). At PET3 the mean average SUVmax was 0.71 with a median of 0 (range 0–3.9). In this series of patients we identified a significant reduction between PET1 and PET2 (P=0.015), between PET2 and PET3 (P=0.006) and between PET1 and PET3 (P=0.0002) (Figure 4B).

Figure 4

Measurment of responses with (A) Example images of 18FDG PET/CT scan obtained from the same patient before (PET1) and after treatment (PET3). A change in the metabolic activity of the tumour between baseline and end of the treatment is clearly observed. (B) Tumour SUVmax values by the 18FDG PET/CT scan. The trend of the tumour metabolic activity during treatment for each patient. A significant reduction in SUVmax has been quantified in all thirteen patients from baseline to the definitive surgery (P-value: between PET1 and PET2 (P=0.015), between PET2 and PET3, (P=0.006) and between PET1 and PET3 (P=0.0002)).

We also monitored the SUVmax in the lymph nodes. At baseline, 10 out of 13 patients presented a positive uptake suggesting tumour involvement of lymph nodes. After 1 month of treatment, seven patients showed a reduction in SUVmax values in the lymph nodes. By the end of treatment, all of the patients had a complete metabolic response in the lymph nodes and in the remaining patients the lymph nodes still showed lower metabolic activity compared with baseline. The average of the SUVmax at PET1 was 2.89 (median=2.50, range 1.1–7.1); at PET2 it was 1.52 (median=1.30, range 0–3.3) and at PET3 it was 0.17 (median=0, range 0–1.7). A significant reduction of SUVmax in the lymph nodes was therefore observed between PET1 and PET2 (P=0.045), PET2 and PET3 (P=0.0008), and PET1 and PET3 (P=0.0002). Using the Modified CT Criteria (Choi ), to evaluate response at 30 days and response just before surgery, our study showed response (CR or PR) in 8 patients and 2 patients with stable disease (n=10 evaluable patients) at 30 days and a response in 11 patients (n=11 evaluable patients) at definitive surgery (Table 3).

Table 3

Response scored using Modified CT Criteria

	Number of patients (%)
	CR	PR	SD	PD
30 days time pointa	1	7	2	0
Definitive surgery time pointb	7	4	0	0

Abbreviations: CR=complete response; PD=progressive disease; PR=partial response; SD=stable disease.

(n=13 patients; in 3 out of 13 patients data were not available).

(n=13 patients; in 2 out of 13 patients data were not available).

Assessment of biological markers

Biological markers were assessed in samples obtained at baseline, at 14 days and at definitive surgery (Figure 1B). Ki67 labelling index was assessable in 11 of the 13 patients, but there was insufficient tissue available for the remaining two patients. At baseline the mean average Ki67 index was 20.69% with median of 20% (range 8–36), at day 14 the mean was 10.64% with median of 10% (range 1–20) and at definitive surgery the mean was 11.38% with median of 7% (range 0–34) (Figure 5A). We observed a marked suppression of the Ki67 proliferation index after 14 days in the 11 patients that could be evaluated (P<0.00001), and there was a significant reduction in Ki67 index at definitive surgery compared with baseline (P=0.03). However, 4 patients out of the 11 evaluated showed an increase in Ki67 index at definitive surgery. At the end of treatment, these four patients showed a clinical partial response, which corresponded to a pT2 and pT4 at the pathology report. It is possible that these four patients experienced early-onset resistance to the therapeutic regimen.

Figure 5

Analysis of molecular markers. (A) Ki67 labelling index at baseline, day 14 and at the definitive surgery. Proliferation trend is reported for each patient. A significant reduction in mean average proliferation index is observed between baseline and day 14 (P=0.00001) and between baseline and end of treatment (P=0.031). (B) Immunohistochemistry demonstrating the expression of angiogenesis-related markers (CD31 and VEGF-A) in pre-treatment and post-treatment samples.

Change in expression of the angiogenesis-related markers CD31 and VEGF-A was also evaluated (Figure 5B). CD31 expression could be evaluated in 10 of the 13 patients at baseline (median expression=3, range 1–5), in 8 out of 13 at day 14 (median expression=1, range 1–3) and in 8 out of 13 at definitive surgery (median expression=1, range 1–3). The CD31 expression showed a significant reduction at day 14 (P=0.006) and at definitive surgery (P=0.01) compared with baseline. VEGF-A expression could be evaluated in 9 of the 13 patients at baseline (median expression=2, range 1–2), in 8 out of 13 at day 14 (median expression=1, range 1–3) and in 9 out of 13 at definitive surgery (median expression=1, range 1–3). No significant difference in VEGF-A expression was found between baseline and day 14, but a significant reduction at the definitive surgery compared with baseline was observed (P=0.007) (Figure 5B). A correlation was observed between the decrease in CD31 and the decrease in VEGF-A expression, both at day 14 (P<0.0001) and at the definitive surgery (P<0.0001).

Discussion

Endocrine resistance presents a major challenge in the management of ER+ve breast cancer and is an area under intense investigation. Non-genomic cross-talk between ER and growth factor receptors can activate intracellular signalling cascades involved in oestrogen-independent growth, such as the Ras-Raf-mitogen-activated protein kinase (MAPK) (Massarweh ) and the phosphatidylinositol-3-kinase (PI3K)/AKT/mTOR pathways (Johnston ). Moreover, both MAPK and AKT can directly enhance the classic genomic transcriptional activity of ER in the absence of oestrogens (Le Goff ) further contributing to endocrine resistance. It has been shown that letrozole-based treatment is able to significantly reduce PI3K and phospho-mTOR expression (Generali ) and that HIF-1alpha and p44/42 MAPK expression is independent factor for endocrine resistance in neoadjuvant setting (Generali ). These data suggest that targeting these pathways could potentiate the aromatase inhibitor effect, preventing or postponing the onset of endocrine resistance. Therefore, combined inhibition of the oestroge pathway and growth factor signalling represents an attractive target for pharmacological intervention. Sorafenib is an oral multikinase inhibitor, capable of inhibiting mTOR signalling and HIF-1alpha expression (Kumar ; Huynh ) or MAPK expression and related proliferation proteins (Keswani ). Our previous in vitro experiments with the combination of letrozole and sorafenib demonstrated a potential role for this combination in the treatment of hormone-dependent BC cells (Bonelli ). However, to date, the combination of sorafenib with letrozole and metronomic administration of cyclophosphamide has never been tested in a clinical setting. We have therefore investigated the pharmacology, toxicity and efficacy of this regimen in primary breast cancer patients.

Pharmacokinetic analysis showed there was no impact of the combination regimen on the circulating levels of sorafenib in patients. Moreover, the analysis of the pharmacokinetic data showed that there was no relevant impact of sorafenib (400 mg BID) on the pharmacokinetics of cyclophosphamide when these compounds were co-administered. However, the data do indicate that concomitant sorafenib moderately decreased the exposure (Cmax and AUC0–24) to cyclophosphamide, without being influenced by the sorafenib total dose. Indeed it is recognised that cyclophosphamide is a potent inducer of microsomal enzymes and is able to increase levels of CYP2C8, 2C9 and 3A4 inducing its own metabolism (Chang ). As previously shown, a similar induction by cyclophosphamide of human CYP2B6 and 3A4 enzyme expression occurs (Gervot ; Lindley ). This auto-induction in cyclophosphamide metabolism is detectable within 24 h after the start of the treatment and might explain the reduction of cyclophosphamide plasma concentrations. However, further investigation in a larger population of cancer patients and a more accurate evaluation of all cyclophosphamide-derived metabolites is warranted.

The metronomic administration of chemotherapy compounds is a good treatment option for breast cancer patients, has a low-toxicity profile and high efficacy in most patients (Montagna ). We previously demonstrated that co-administration of endocrine therapy with the metronomic administration of cyclophosphamide is well tolerated without any particular adverse events (Bottini ).

In the current study, we show that sorafenib administered with cyclophosphamide and letrozole had a safety profile consistent with that of the individual agents in patients with breast cancer. The most common toxicities were skin rash, hand-foot skin reaction and diarrhea Although all are reported to be associated with sorafenib (Gomez & Lacouture, 2011), it is difficult to determine whether sorafenib was the sole agent responsible for the relative high incidence of these events among the enrolled patients. Across three phase 2 randomised studies where advanced breast cancer patients were treated with sorafenib in combination with chemotherapy (Baselga ; Gradishar ; Schwartzberg ), the incidence of hand-foot syndrome, rash and diarrhea was 31–44, 2–4 and 3–6%, respectively. By comparison, the incidence of hand-foot syndrome (69.3%), rash (69.3%) and diarrhea (46.1%) was elevated in our study. On the contrary, the incidence of other grade 3/4 toxicities reported in our study, such as fatigue and neutropenia, was similar to those reported in these previous studies that combined chemotherapy with sorafenib. However, despite this incidence of toxicity, in our experience the timely detection of adverse events in patients helps to mitigate their clinical consequences.

It is also of interest to compare the toxicity profile of LCS with a previous study that combined endocrine therapy with sorafenib in breast cancer. Isaacs reported a phase I/II study that employed a combination of sorafenib and anastrazole in patients with metastatic breast cancer. In that study, 77% of patients required dose reductions of sorafenib and 31% came off study due to toxicity. The most common grade 3/4 toxicities were hand-foot syndrome (34%), fatigue (17%), rash (11%), emesis (11%) and hypertension (11%). Among the 35 women enrolled into that phase I/II trial, 46% had an ECOG ⩾1, 23% had visceral metastasis, 43% had bone metastasis and all had received one or two lines of previous therapy. In contrast, the women enrolled in our study were naive of any treatment and without any visceral/bone involvement. These differences in the patient populations may explain the different toxicity profile reported by our group in comparison with the study by Isaacs .

Clinical anti-cancer activity (complete and partial responses) was observed in all patients. This was objectively confirmed by the 18FDG-PET/CT scan, with decreased SUVmax values observed in all 13 patients after treatment. However, FDG PET/CT failed to detect 11 instances of isolated tumour cells, micro-embolic or plural-embolic in axillary nodes, which were reported in the definitive pathological report. In some studies the role of FDG PET/CT in the axillary staging of breast cancer has been questioned with FDG PET/CT proving to have poor sensitivity compared with immunohistochemistry on lymph nodes biopsy in which multistep sectioning is commonly used to identify axillary micrometastases (that is, clusters of malignant cells >0.2 mm to ⩽2.0 mm in diameter) (Guller ; Kelemen ; van der Hoeven ). Thus, the limited spatial resolution of the current FDG PET/CT scanners in comparison with the more accurate pathological examination techniques is a reason for these modest results of PET in the detection of micro-metastases.

The Ki67 labelling index after 2 weeks of therapy and at definitive surgery was significantly lower than the labelling index at the baseline for eight patients. In particular, we noticed that the reduction of Ki67 expression was greater between the beginning and the end of the treatment than after 15 days of treatment. Taken together, these data suggest that the tried and tested letrozole-cyclophosphamide combination (Bottini ) with sorafenib is promising in terms of metabolic activity and suppression proliferation index. Of note, however, are four patients that did not show a reduction in tumour proliferation activity at the end of the treatment. As previously described by Dowsett , this might be an indicator of endocrine resistance. However, Ki67 may not be the most reliable indicator of tumour cell proliferation. As Dowsett have proposed, to better investigate the anti-proliferative effect of therapy, it might be more appropriate to analyse other indices of cell proliferation, such as S phase markers or MCM2.

Previous reports showed the metronomic schedule is able to modulate several factors, such as VEGF-A, circulating endothelial cells and fibroblast growth factor 2, which are all involved in remodelling of cancer vessels (Mancuso ; Calleri ; Isaacs ). Our group has shown that the combination of letrozole and metronomic cyclophosphamide has an anti-angiogenic effect with a significant reduction of VEGF-A after 6 months of neoadjuvant treatment (Bottini ). Here we investigated the expression of two robust markers, CD31 and VEGF-A, supported also by published data on their modulation and their correlation with treatment response to bevacizumab (Yang ). The expression of CD31 and VEGF-A was significantly decreased after treatment, supporting the contention that the administered regimen has an anti-angiogenic effect in primary BC.

In this study, we also used the breast MRI technique as a more accurate method than breast ultrasound and mammography or clinical palpation (Bodini ) in evaluating the disease response to therapy and because of its ability to delineate anatomic detail of breast cancers. In our study, there was only a modest evidence of significant changes in tumour dimensions after 14 days of treatment, maybe due to the short interval between the two evaluations. In the future, it would be preferable to perform the breast MRI at time points further into treatment to monitor properly any differences in clinical response induced by the treatment (Bodini ). Moreover, improvements in an MRI technology for the monitoring of blood-flow as a marker of tumour angiogenesis (Mehta ) are warranted in future trials containing anti-angiogenic drugs such as sorafenib and or metronomic cyclophosphamide.

In conclusion, the addition of sorafenib to the already tested letrozole-cyclophosphamide combination was associated with a clinical response, a significant decrease in tumour metabolic activity, in proliferation index and in angiogenesis in patients with hormone receptor positive primary breast cancer. Conceivably, this benefit may be due to the ability of sorafenib to overcome resistance to endocrine therapy and/or the increased anti-angiogenic activity when sorafenib is combined with the metronomic administration of cyclophosphamide.