Literature DB >> 25461484

Tetracycline-inducible gene expression system in Leishmania mexicana.

Natalya Kraeva1, Aygul Ishemgulova1, Julius Lukeš2, Vyacheslav Yurchenko3.   

Abstract

Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The gene expression in this strain is tightly regulated and dose- and time-dependent. This system can be widely used by the parasitology community to analyze effects of genes of interest on biology, physiology and virulence of parasites causing cutaneous leishmaniases.
Copyright © 2014 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Gene expression; Leishmania mexicana; Tet-inducible system

Mesh：

Substances：

Year:  2014        PMID： 25461484     DOI： 10.1016/j.molbiopara.2014.11.002

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN： 0166-6851            Impact factor:   1.759


  13 in total

1.  Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content.

Authors:  Alexandra Zakharova; Amanda T S Albanaz; Fred R Opperdoes; Ingrid Škodová-Sveráková; Diana Zagirova; Andreu Saura; Lˇubomíra Chmelová; Evgeny S Gerasimov; Tereza Leštinová; Tomáš Bečvář; Jovana Sádlová; Petr Volf; Julius Lukeš; Anton Horváth; Anzhelika Butenko; Vyacheslav Yurchenko
Journal:  PLoS Negl Trop Dis       Date:  2022-06-24

2.  Leptomonas seymouri: Adaptations to the Dixenous Life Cycle Analyzed by Genome Sequencing, Transcriptome Profiling and Co-infection with Leishmania donovani.

Authors:  Natalya Kraeva; Anzhelika Butenko; Jana Hlaváčová; Alexei Kostygov; Jitka Myškova; Danyil Grybchuk; Tereza Leštinová; Jan Votýpka; Petr Volf; Fred Opperdoes; Pavel Flegontov; Julius Lukeš; Vyacheslav Yurchenko
Journal:  PLoS Pathog       Date:  2015-08-28       Impact factor: 6.823

Review 3.  Trypanosome Mitochondrial Translation and Tetracycline: No Sweat about Tet.

Authors:  Hassan Hashimi; Sabine Kaltenbrunner; Alena Zíková; Julius Lukeš
Journal:  PLoS Pathog       Date:  2016-04-21       Impact factor: 6.823

Review 4.  Plasticity of the Leishmania genome leading to gene copy number variations and drug resistance.

Authors:  Marie-Claude N Laffitte; Philippe Leprohon; Barbara Papadopoulou; Marc Ouellette
Journal:  F1000Res       Date:  2016-09-20

5.  A putative ATP/GTP binding protein affects Leishmania mexicana growth in insect vectors and vertebrate hosts.

Authors:  Aygul Ishemgulova; Natalya Kraeva; Jana Hlaváčová; Sara L Zimmer; Anzhelika Butenko; Lucie Podešvová; Tereza Leštinová; Julius Lukeš; Alexei Kostygov; Jan Votýpka; Petr Volf; Vyacheslav Yurchenko
Journal:  PLoS Negl Trop Dis       Date:  2017-07-24

6.  A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids.

Authors:  Tom Beneke; Ross Madden; Laura Makin; Jessica Valli; Jack Sunter; Eva Gluenz
Journal:  R Soc Open Sci       Date:  2017-05-03       Impact factor: 2.963

7.  CRISPR/Cas9 in Leishmania mexicana: A case study of LmxBTN1.

Authors:  Aygul Ishemgulova; Jana Hlaváčová; Karolina Majerová; Anzhelika Butenko; Julius Lukeš; Jan Votýpka; Petr Volf; Vyacheslav Yurchenko
Journal:  PLoS One       Date:  2018-02-13       Impact factor: 3.240

8.  Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections.

Authors:  Tom Beneke; François Demay; Edward Hookway; Nicole Ashman; Heather Jeffery; James Smith; Jessica Valli; Tomas Becvar; Jitka Myskova; Tereza Lestinova; Shahaan Shafiq; Jovana Sadlova; Petr Volf; Richard John Wheeler; Eva Gluenz
Journal:  PLoS Pathog       Date:  2019-06-26       Impact factor: 6.823

9.  Suicidal Leishmania.

Authors:  Lucie Podešvová; Tereza Leštinová; Eva Horáková; Julius Lukeš; Petr Volf; Vyacheslav Yurchenko
Journal:  Pathogens       Date:  2020-01-25

10.  Simplified inducible system for Trypanosoma brucei.

Authors:  Gabriela T Niemirowicz; Juan J Cazzulo; Vanina E Álvarez; León A Bouvier
Journal:  PLoS One       Date:  2018-10-11       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.