Preeti Chhabra1, Elena Samoilovich2, Marina Yermalovich2, Liudmyla Chernyshova3, Stela Gheorghita4, Radu Cojocaru4, Nazim Shugayev5, Gayane Sahakyan6, Marina Lashkarashvili7, Marina Chubinidze7, Khatuna Zakhashvili7, Dovile Videbaek8, Annemarie Wasley8, Jan Vinjé9. 1. Division of Viral Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States. Electronic address: pchhabra@cdc.gov. 2. Republican Research and Practical Center for Epidemiology and Microbiology, Ministry of Health, Minsk, Belarus. 3. Department of Pediatric Infectious Diseases and Clinical Immunology of National Academy for Post-graduate Education, Kiev, Ukraine. 4. National Center for Public Health, Chişinău, Republic of Moldova. 5. Azerbaijan Republican Anti-plague Station, Baku, Azerbaijan. 6. National Center of Disease Control and Prevention, Ministry of Health, Yerevan, Armenia. 7. National Center for Disease Control and Public Health, Tbilisi, Georgia. 8. WHO Regional Office for Europe, Vaccine-preventable Diseases & Immunization, Copenhagen, Denmark. 9. Division of Viral Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States.
Abstract
PURPOSE: Rotavirus causes nearly 40% of all hospitalizations for AGE among children <5 years of age in the NIS of the former Soviet Union. The etiologic role of other established gastroenteritis viruses in this age group is unknown. METHODS: Laboratory-confirmed rotavirus negative fecal specimens (N=495) collected between January and December 2009 from children in 6 NIS (Armenia, Azerbaijan, Belarus, Georgia, Republic of Moldova and Ukraine) were tested for norovirus, sapovirus, enteric adenovirus and astrovirus by real-time RT-PCR. Genotyping was carried out by sequencing and phylogenetic analysis. RESULTS: Norovirus, enteric adenovirus, sapovirus and astrovirus were detected in 21.8%, 4.0%, 3.2%, and 1.4% of the rotavirus negative specimens, respectively. Mixed infections were identified in 4.1% of the specimens. Phylogenetic analysis showed co-circulation of several different genotypes with GII.4 Den Haag (2006b) norovirus, GI.2 sapovirus, adenovirus type 41, and astrovirus type 1 causing majority of the infections. CONCLUSION: Norovirus, enteric adenovirus, sapovirus and astrovirus account for a significant proportion (30.5%) of AGE in hospitalized children <5 years of age in 6 NIS.
PURPOSE: Rotavirus causes nearly 40% of all hospitalizations for AGE among children <5 years of age in the NIS of the former Soviet Union. The etiologic role of other established gastroenteritis viruses in this age group is unknown. METHODS: Laboratory-confirmed rotavirus negative fecal specimens (N=495) collected between January and December 2009 from children in 6 NIS (Armenia, Azerbaijan, Belarus, Georgia, Republic of Moldova and Ukraine) were tested for norovirus, sapovirus, enteric adenovirus and astrovirus by real-time RT-PCR. Genotyping was carried out by sequencing and phylogenetic analysis. RESULTS: Norovirus, enteric adenovirus, sapovirus and astrovirus were detected in 21.8%, 4.0%, 3.2%, and 1.4% of the rotavirus negative specimens, respectively. Mixed infections were identified in 4.1% of the specimens. Phylogenetic analysis showed co-circulation of several different genotypes with GII.4 Den Haag (2006b) norovirus, GI.2 sapovirus, adenovirus type 41, and astrovirus type 1 causing majority of the infections. CONCLUSION: Norovirus, enteric adenovirus, sapovirus and astrovirus account for a significant proportion (30.5%) of AGE in hospitalized children <5 years of age in 6 NIS.
Authors: Serhii O Soloviov; Tetiana S Todosiichuk; Olena V Kovaliuk; Gabriel M Filippelli; Olena P Trokhymenko; Iryna V Dziublyk; Zachary A Rodd Journal: Int J Environ Res Public Health Date: 2022-04-12 Impact factor: 4.614
Authors: Joana Rocha-Pereira; Abimbola O Kolawole; Eric Verbeken; Christiane E Wobus; Johan Neyts Journal: Antiviral Res Date: 2016-05-29 Impact factor: 5.970