| Literature DB >> 25459681 |
Debanjan Tewari1, Tofayel Ahmed1, Venkat R Chirasani1, Pradeep K Singh2, Samir K Maji2, Sanjib Senapati1, Amal Kanti Bera3.
Abstract
Voltage dependent anion channel (VDAC) of mitochondria plays a crucial role in apoptosis. Human VDAC-1, reconstituted in planar lipid bilayer showed reduced conductance when treated with curcumin. Curcumin interacts with residues in the α helical N-terminus of VDAC and in the channel wall, as revealed by molecular docking, followed by mutational analysis. N-terminus mimicking peptide showed conformational changes in circular dichroism, upon curcumin treatment. We propose that the interaction of curcumin with amino acids in N-terminus and in channel wall fixes the α helix in closed conformation. This restricts its movement which is required for the opening of the channel.Entities:
Keywords: Apoptosis; Curcumin; Gating; Ion channel; Mitochondria; VDAC
Mesh：
Substances：
Year: 2014 PMID： 25459681 DOI： 10.1016/j.bbamem.2014.10.014
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002