Raquel Sabino1, Jose A G Ferreira2, Richard B Moss3, Joana Valente4, Cristina Veríssimo4, Elisabete Carolino5, Karl V Clemons6, Cassie Everson3, Niaz Banaei7, John Penner8, David A Stevens6. 1. National Institute of Health Dr. Ricardo Jorge-Infectious Diseases Department, Lisbon, Portugal; Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, United States; California Institute for Medical Research, San Jose, CA, United States. Electronic address: raquelsabino@hotmail.com. 2. Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, United States; California Institute for Medical Research, San Jose, CA, United States; School of Medicine, Faculdade da Saúde e Ecologia Humana-FASEH, Vespasiano, Brazil. 3. Department of Pediatrics, Division of Pulmonology, Stanford University, Stanford, CA, United States. 4. National Institute of Health Dr. Ricardo Jorge-Infectious Diseases Department, Lisbon, Portugal. 5. Scientific Area of Mathematics, Lisbon School of Health Technology, Polytechnic Institute of Lisbon, Lisbon, Portugal. 6. Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, United States; California Institute for Medical Research, San Jose, CA, United States. 7. Clinical Microbiology Laboratory, Stanford University, Stanford, CA, United States. 8. California Institute for Medical Research, San Jose, CA, United States.
Abstract
BACKGROUND: Aspergillus respiratory infection is a common complication in cystic fibrosis (CF) and is associated with loss of pulmonary function and allergic disease. METHODS: Fifty-three Aspergillus isolates recovered from CF patients were identified to species by Internal Transcribed Spacer Region (ITS), β-tubulin, and calmodulin sequencing. RESULTS: Three species complexes (Terrei, Nigri, and Fumigati) were found. Identification to species level gave a single Aspergillus terreus sensu stricto, one Aspergillus niger sensu stricto and 51 Aspergillus fumigatus sensu stricto isolates. No cryptic species were found. CONCLUSIONS: To our knowledge, this is the first prospective study of Aspergillus species in CF using molecular methods. The paucity of non-A. fumigatus and of cryptic species of A. fumigatus suggests a special association of A. fumigatus sensu stricto with CF airways, indicating it likely displays unique characteristics making it suitable for chronic residence in that milieu. These findings could refine an epidemiologic and therapeutic approach geared to this pathogen.
BACKGROUND:Aspergillus respiratory infection is a common complication in cystic fibrosis (CF) and is associated with loss of pulmonary function and allergic disease. METHODS: Fifty-three Aspergillus isolates recovered from CF patients were identified to species by Internal Transcribed Spacer Region (ITS), β-tubulin, and calmodulin sequencing. RESULTS: Three species complexes (Terrei, Nigri, and Fumigati) were found. Identification to species level gave a single Aspergillus terreus sensu stricto, one Aspergillus niger sensu stricto and 51 Aspergillus fumigatus sensu stricto isolates. No cryptic species were found. CONCLUSIONS: To our knowledge, this is the first prospective study of Aspergillus species in CF using molecular methods. The paucity of non-A. fumigatus and of cryptic species of A. fumigatus suggests a special association of A. fumigatus sensu stricto with CF airways, indicating it likely displays unique characteristics making it suitable for chronic residence in that milieu. These findings could refine an epidemiologic and therapeutic approach geared to this pathogen.
Authors: Hasan Nazik; Richard B Moss; Vyshnavi Karna; Karl V Clemons; Niaz Banaei; Kevin Cohen; Varun Choudhary; David A Stevens Journal: Mycopathologia Date: 2016-11-07 Impact factor: 2.574
Authors: Gabriele Sass; Laura C Miller Conrad; Terrence-Thang H Nguyen; David A Stevens Journal: Trans R Soc Trop Med Hyg Date: 2020-07-01 Impact factor: 2.184
Authors: Hasan Nazik; John C Penner; Jose A Ferreira; Janus A J Haagensen; Kevin Cohen; Alfred M Spormann; Marife Martinez; Vicky Chen; Joe L Hsu; Karl V Clemons; David A Stevens Journal: Antimicrob Agents Chemother Date: 2015-08-03 Impact factor: 5.191
Authors: David A Stevens; Richard B Moss; Cathy Hernandez; Karl V Clemons; Marife Martinez Journal: Antimicrob Agents Chemother Date: 2016-03-25 Impact factor: 5.191
Authors: Gabriele Sass; Hasan Nazik; John Penner; Hemi Shah; Shajia Rahman Ansari; Karl V Clemons; Marie-Christine Groleau; Anna-Maria Dietl; Paolo Visca; Hubertus Haas; Eric Déziel; David A Stevens Journal: J Bacteriol Date: 2017-12-05 Impact factor: 3.490