| Literature DB >> 25457745 |
David Madariaga1, Nuria Martínez-Sáez1, Víctor J Somovilla1, Helena Coelho1, Jessika Valero-González1, Jorge Castro-López1, Juan L Asensio1, Jesús Jiménez-Barbero1, Jesús H Busto1, Alberto Avenoza1, Filipa Marcelo1, Ramón Hurtado-Guerrero1, Francisco Corzana1, Jesús M Peregrina1.
Abstract
Tn antigen (α-O-GalNAc-Ser/Thr) is a convenient cancer biomarker that is recognized by antibodies and lectins. This work yields remarkable results for two plant lectins in terms of epitope recognition and reveals that these receptors show higher affinity for Tn antigen when it is incorporated in the Pro-Asp-Thr-Arg (PDTR) peptide region of mucin MUC1. In contrast, a significant affinity loss is observed when Tn antigen is located in the Ala-His-Gly-Val-Thr-Ser-Ala (AHGVTSA) or Ala-Pro-Gly-Ser-Thr-Ala-Pro (APGSTAP) fragments. Our data indicate that the charged residues, Arg and Asp, present in the PDTR sequence establish noteworthy fundamental interactions with the lectin surface as well as fix the conformation of the peptide backbone, favoring the presentation of the sugar moiety toward the lectin. These results may help to better understand glycopeptide-lectin interactions and may contribute to engineer new binding sites, allowing novel glycosensors for Tn antigen detection to be designed.Entities:
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Year: 2014 PMID： 25457745 DOI： 10.1021/cb500855x
Source DB: PubMed Journal: ACS Chem Biol ISSN： 1554-8929 Impact factor: 5.100