Guang-yu Chen1, Rui-wu Dai1, Hao Luo1, Wei-hui Liu1, Tao Chen1, Ning Lin1, Tao Wang1, Guo-de Luo1, Li-jun Tang2. 1. General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China. 2. General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China. Electronic address: acecgy@gmail.com.
Abstract
OBJECTIVE: This study investigated the effect of percutaneous catheter drainage (PCD) on pancreatic injury in severe acute pancreatitis (SAP) rats. METHODS: Sixty Wistar rats were equally randomized into three groups: a sham operated control group, an SAP control group, and a PCD group. The levels of inflammatory cytokines, the activity of group II phospholipase A2 (PLA2) in blood and ascitic fluid, and the pancreas level of group II PLA2 and trypsin activity were measured 24 h after the operation. The apoptosis of the pancreatic cells, the expression of cycloxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), active caspase-3, Bcl-2 and Bax in the pancreas was detected. Pancreatic pathological changes were observed. RESULTS: The levels of proinflammatory cytokines, the activity of group II PLA2 and trypsin activity in pancreas in the SAP group were higher than those in the PCD group. The histopathological results revealed that the pancreatic injury was alleviated in the PCD group. The expression of COX-2 and iNOS in the pancreatic tissue in the SAP control rats was higher than that in the PCD rats. The expression of Bcl-2 was decreased and the expression of active caspase-3 and Bax was increased in the pancreas of PCD rats. The apoptosis index of the pancreatic cells in the PCD rats was higher than that in the SAP control rats. CONCLUSION: PCD can relieve SAP-induced pancreatic injury by inhibiting inflammatory reactions, and promoting apoptosis of pancreatic cells.
OBJECTIVE: This study investigated the effect of percutaneous catheter drainage (PCD) on pancreatic injury in severe acute pancreatitis (SAP) rats. METHODS: Sixty Wistar rats were equally randomized into three groups: a sham operated control group, an SAP control group, and a PCD group. The levels of inflammatory cytokines, the activity of group II phospholipase A2 (PLA2) in blood and ascitic fluid, and the pancreas level of group II PLA2 and trypsin activity were measured 24 h after the operation. The apoptosis of the pancreatic cells, the expression of cycloxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), active caspase-3, Bcl-2 and Bax in the pancreas was detected. Pancreatic pathological changes were observed. RESULTS: The levels of proinflammatory cytokines, the activity of group II PLA2 and trypsin activity in pancreas in the SAP group were higher than those in the PCD group. The histopathological results revealed that the pancreatic injury was alleviated in the PCD group. The expression of COX-2 and iNOS in the pancreatic tissue in the SAP control rats was higher than that in the PCDrats. The expression of Bcl-2 was decreased and the expression of active caspase-3 and Bax was increased in the pancreas of PCDrats. The apoptosis index of the pancreatic cells in the PCDrats was higher than that in the SAP control rats. CONCLUSION:PCD can relieve SAP-induced pancreatic injury by inhibiting inflammatory reactions, and promoting apoptosis of pancreatic cells.
Authors: Jolanta Jaworek; Anna Leja-Szpak; Katarzyna Nawrot-Porąbka; Joanna Szklarczyk; Michalina Kot; Piotr Pierzchalski; Marta Góralska; Piotr Ceranowicz; Zygmunt Warzecha; Artur Dembinski; Joanna Bonior Journal: Int J Mol Sci Date: 2017-05-08 Impact factor: 5.923
Authors: Jolanta Jaworek; Barbara Tudek; Paweł Kowalczyk; Michalina Kot; Joanna Szklarczyk; Anna Leja-Szpak; Piotr Pierzchalski; Joanna Bonior; Artur Dembiński; Piotr Ceranowicz; Zygmunt Warzecha; Katarzyna Nawrot-Porąbka; Krzysztof Gil Journal: Gastroenterol Res Pract Date: 2018-01-14 Impact factor: 2.260