| Literature DB >> 25453291 |
Jie Liu1, Xi Chen1, Chanjuan Yue1, Ranran Hou1, Jin Chen2, Yu Lu2, Xiuping Li1, Rongjia Li1, Cui Liu1, Zhenzhen Gao1, Entao Li1, Youying Li1, Han Wang1, Yue Yan1, Hongquan Li3, Yuanliang Hu4.
Abstract
The Atractylodes macrocephala polysaccharide (AMP) was extracted purified and modified in selenylation by Nitric acid-sodium selenite method to get nine selenizing AMPs (sAMPs), sAMP(1)-sAMP(9). In vitro test their effects on chicken peripheral lymphocyte proliferation were determined by MTT assay. The results showed that nine sAMPs and AMP at five concentrations could significantly promote lymphocyte proliferation, the actions in six sAMPs were significantly stronger than that in AMP, and in sAMP(9) was the strongest. In vivo test, 14-day-old chickens vaccinated with ND vaccine were injected respectively with sAMP(9) and AMP, the peripheral lymphocytes proliferation, serum antibody titer, IFN-γ, IL-2 and IL-6 contents were determined. The results displayed that the sAMP could significantly promote lymphocyte proliferation and elevate the antibody titers and content of IFN-γ, IL-2 and IL-6 in comparison with unmodified AMP. These results indicate that selenylation modification can significantly enhance the immune-enhancing activity of AMP.Entities:
Keywords: Atractylodes macrocephala polysaccharide; Immune-enhancing activity; Selenylation modification
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Year: 2014 PMID： 25453291 DOI： 10.1016/j.ijbiomac.2014.10.022
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953