Kuang-Ming Chen1, Ming-Ko Chiang2, Meilin Wang3, Han-Chen Ho4, Min-Chi Lu5, Yi-Chyi Lai6. 1. Department of Infectious Disease, Antai Medical Care Cooperation Antai Tian-Sheng Memorial Hospital, Ping-tung, Taiwan, ROC. Electronic address: cowcutty@yahoo.com.tw. 2. Department of Life Science, National Chung Cheng University, Chia-Yi, Taiwan, ROC. Electronic address: mkochiang@yahoo.com. 3. Department of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan, ROC. Electronic address: wml@csmu.edu.tw. 4. Department of Anatomy, Tzu Chi University, Hualien, Taiwan, ROC. Electronic address: hcho@mail.tcu.edu.tw. 5. Department of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan, ROC; Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan, ROC. Electronic address: luminchi@csmu.edu.tw. 6. Department of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan, ROC; Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan, ROC. Electronic address: yclai@csmu.edu.tw.
Abstract
BACKGROUND: Klebsiella pneumoniae has emerged as one of the major pathogens for community-acquired and nosocomial infections. A four-gene locus that had a high degree similarity with Escherichia coli pgaABCD and Yersinia pestis hmsHFRS was identified in K. pneumoniae genomes. The pgaABCD in E. coli encodes the envelope-spanning Pga machinery for the synthesis and secretion of poly-β-linked N-acetylglucosamine (PNAG). In a limited number of phylogenetically diverse bacteria, PNAG was demonstrated to mediate biofilm formation and had a role in the host-bacteria interactions. The presence of conserved pgaABCD locus among various K. pneumoniae strains suggested a putative requirement of PNAG for this bacterium. RESULTS: In this study, an in-frame deletion of pgaC was generated in K. pneumoniae CG43 and named ΔpgaC. The loss of pgaC affected the production of PNAG and attenuated the enhancement of in vitro biofilm formation upon the addition of bile salts mixture. In mouse models, ΔpgaC exhibited a weakened ability to colonize the intestine, to disseminate extraintestinally, and to induce a systemic infection when compared to K. pneumoniae CG43. CONCLUSIONS: Our study demonstrated that pgaC participated in the bile salts induced biofilm formation and was required for K. pneumoniae virulence in vivo.
BACKGROUND:Klebsiella pneumoniae has emerged as one of the major pathogens for community-acquired and nosocomial infections. A four-gene locus that had a high degree similarity with Escherichia colipgaABCD and Yersinia pestis hmsHFRS was identified in K. pneumoniae genomes. The pgaABCD in E. coli encodes the envelope-spanning Pga machinery for the synthesis and secretion of poly-β-linked N-acetylglucosamine (PNAG). In a limited number of phylogenetically diverse bacteria, PNAG was demonstrated to mediate biofilm formation and had a role in the host-bacteria interactions. The presence of conserved pgaABCD locus among various K. pneumoniae strains suggested a putative requirement of PNAG for this bacterium. RESULTS: In this study, an in-frame deletion of pgaC was generated in K. pneumoniae CG43 and named ΔpgaC. The loss of pgaC affected the production of PNAG and attenuated the enhancement of in vitro biofilm formation upon the addition of bile salts mixture. In mouse models, ΔpgaC exhibited a weakened ability to colonize the intestine, to disseminate extraintestinally, and to induce a systemic infection when compared to K. pneumoniae CG43. CONCLUSIONS: Our study demonstrated that pgaC participated in the bile salts induced biofilm formation and was required for K. pneumoniae virulence in vivo.
Authors: Kevin S Gipson; Kourtney P Nickerson; Eliana Drenkard; Alejandro Llanos-Chea; Snaha Krishna Dogiparthi; Bernard B Lanter; Rhianna M Hibbler; Lael M Yonker; Bryan P Hurley; Christina S Faherty Journal: Infect Immun Date: 2020-09-18 Impact factor: 3.441
Authors: Maria Magana; Christina Sereti; Anastasios Ioannidis; Courtney A Mitchell; Anthony R Ball; Emmanouil Magiorkinis; Stylianos Chatzipanagiotou; Michael R Hamblin; Maria Hadjifrangiskou; George P Tegos Journal: Clin Microbiol Rev Date: 2018-04-04 Impact factor: 26.132
Authors: Graham G Willsey; Sebastian Ventrone; Kristin C Schutz; Aaron M Wallace; John W Ribis; Benjamin T Suratt; Matthew J Wargo Journal: Infect Immun Date: 2018-06-21 Impact factor: 3.441