| Literature DB >> 25450789 |
Antoinette A Westen1, Thirsa Kraaijenbrink2, Lindy Clarisse3, Laurens J W Grol4, Patricia Willemse5, Sofia B Zuniga6, Elizaveta A Robles de Medina7, Ron Schouten8, Kristiaan J van der Gaag9, Natalie E C Weiler10, Arnoud J Kal11, Manfred Kayser12, Titia Sijen13, Peter de Knijff14.
Abstract
The genotypes of 36 Y-chromosomal short tandem repeat (Y-STR) marker units were analysed in a Dutch population sample of 2085 males. Profiling results were compared for several partially overlapping kits, i.e. PowerPlex Y, Yfiler, PowerPlex Y23, and two in-house designed multiplexes with rapidly mutating Y-STRs. Nineteen Y-STR marker units, of which two are rapidly mutating, reside in at least two of these multiplexes, and for these markers concordance testing was performed. Two samples showed discordant genotyping results and the probable causative base change was revealed by Sanger sequencing. In addition, we encountered concordant, but aberrant genotyping results including one allele with low peak height and several null alleles. For 12 samples, this involved a null allele in two adjacent loci suggesting a large and recurrent deletion as the samples represent three distinct haplogroups. For each marker unit, the allele counts and frequencies are presented, as are the haplotype counts and haplotype diversities for several combinations of markers.Keywords: Allele frequency; Concordance study; Forensic science; Haplotype diversity; Rapidly mutating Y-STR marker unit; Sanger sequencing
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Year: 2014 PMID： 25450789 DOI： 10.1016/j.fsigen.2014.10.012
Source DB: PubMed Journal: Forensic Sci Int Genet ISSN： 1872-4973 Impact factor: 4.882