| Literature DB >> 25448362 |
Michal Jurášek1, Silvie Rimpelová2, Vladimíra Pavlíčková2, Tomáš Ruml2, Oldřich Lapčík1, Pavel B Drašar3.
Abstract
Here, we report synthesis and biological evaluation of fluorescent nandrolone-3-carboxymethyloxime derivatives conjugated with green-emitting bodipy dye via PEG linkers. All the newly-synthesized compounds were evaluated for their effect on cell proliferation in vitro in MCF-7, LNCaP, PC-3 and HEK 293T model cell lines using WST-1 assay. By means of live-cell fluorescence microscopy, the intracellular localization of nandrolone-bodipy conjugates was revealed in endoplasmic reticulum. Moreover, we performed competitive localization study with nonfluorescent nandrolone, metandrolone, boldenone, trenbolone, and testosterone.Entities:
Keywords: Androgens; Cytotoxicity; Fluorescent conjugates; Nandrolone
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Year: 2014 PMID： 25448362 DOI： 10.1016/j.steroids.2014.10.002
Source DB: PubMed Journal: Steroids ISSN： 0039-128X Impact factor: 2.668