Literature DB >> 25447866

Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins.

Joanne E Nettleship1, Peter J Watson, Nahid Rahman-Huq, Louise Fairall, Mareike G Posner, Abhishek Upadhyay, Yamini Reddivari, Jonathan M G Chamberlain, Simon E Kolstoe, Stefan Bagby, John W R Schwabe, Raymond J Owens.   

Abstract

Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

Entities:  

Mesh：

Substances：

Year:  2015        PMID： 25447866     DOI： 10.1007/978-1-4939-2205-5_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small Scale: Application for Structural Studies.

Authors:  Ganna O Krasnoselska; Maud Dumoux; Nadisha Gamage; Harish Cheruvara; James Birch; Andrew Quigley; Raymond J Owens
Journal:  Methods Mol Biol       Date:  2021

2.  Heterologous expression of plant glycosyltransferases for biochemistry and structural biology.

Authors:  Pradeep K Prabhakar; Hsin-Tzu Wang; Peter J Smith; Jeong-Yeh Yang; William J Barnes; Maria J Peña; Kelley W Moremen; Breeanna R Urbanowicz
Journal:  Methods Cell Biol       Date:  2020-06-13       Impact factor: 1.441

3.  ENPP1 processes protein ADP-ribosylation in vitro.

Authors:  Luca Palazzo; Casey M Daniels; Joanne E Nettleship; Nahid Rahman; Robert Lyle McPherson; Shao-En Ong; Kazuki Kato; Osamu Nureki; Anthony K L Leung; Ivan Ahel
Journal:  FEBS J       Date:  2016-08-05       Impact factor: 5.542

Review 4.  Methods for Using Small Non-Coding RNAs to Improve Recombinant Protein Expression in Mammalian Cells.

Authors:  Sarah Inwood; Michael J Betenbaugh; Joseph Shiloach
Journal:  Genes (Basel)       Date:  2018-01-09       Impact factor: 4.096

5.  Neutralization of SARS-CoV-2 by Destruction of the Prefusion Spike.

Authors:  Jiandong Huo; Yuguang Zhao; Jingshan Ren; Daming Zhou; Helen M E Duyvesteyn; Helen M Ginn; Loic Carrique; Tomas Malinauskas; Reinis R Ruza; Pranav N M Shah; Tiong Kit Tan; Pramila Rijal; Naomi Coombes; Kevin R Bewley; Julia A Tree; Julika Radecke; Neil G Paterson; Piyada Supasa; Juthathip Mongkolsapaya; Gavin R Screaton; Miles Carroll; Alain Townsend; Elizabeth E Fry; Raymond J Owens; David I Stuart
Journal:  Cell Host Microbe       Date:  2020-06-19       Impact factor: 21.023

6.  Optimized Recombinant Production of Secreted Proteins Using Human Embryonic Kidney (HEK293) Cells Grown in Suspension.

Authors:  Silvia Faravelli; Matteo Campioni; Martina Palamini; Anselmo Canciani; Antonella Chiapparino; Federico Forneris
Journal:  Bio Protoc       Date:  2021-04-20

7.  The structure of the core NuRD repression complex provides insights into its interaction with chromatin.

Authors:  Christopher J Millard; Niranjan Varma; Almutasem Saleh; Kyle Morris; Peter J Watson; Andrew R Bottrill; Louise Fairall; Corinne J Smith; John W R Schwabe
Journal:  Elife       Date:  2016-04-21       Impact factor: 8.140

Review 8.  Protein stability: a crystallographer's perspective.

Authors:  Marc C Deller; Leopold Kong; Bernhard Rupp
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2016-01-26       Impact factor: 1.056

9.  Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1.

Authors:  Victoria Arena de Souza; David J Scott; Joanne E Nettleship; Nahid Rahman; Michael H Charlton; Martin A Walsh; Raymond J Owens
Journal:  PLoS One       Date:  2015-12-11       Impact factor: 3.240

10.  Diversity between mammalian tolloid proteinases: Oligomerisation and non-catalytic domains influence activity and specificity.

Authors:  Christopher P Bayley; Hilda D Ruiz Nivia; Rana Dajani; Thomas A Jowitt; Richard F Collins; Heather Rada; Louise E Bird; Clair Baldock
Journal:  Sci Rep       Date:  2016-02-23       Impact factor: 4.379
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.