| Literature DB >> 25447857 |
Agustín Correa1, Pablo Oppezzo.
Abstract
Despite the importance of recombinant protein production in academy and industrial fields, many issues concerning the expression of soluble and homogeneous product are still unsolved. Although several strategies were developed to overcome these obstacles, at present there is no magic bullet that can be applied for all cases. Indeed, several key expression parameters need to be evaluated for each protein. Among the different hosts for protein expression, Escherichia coli is by far the most widely used. In this chapter, we review many of the different tools employed to circumvent protein insolubility problems.Entities:
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Year: 2015 PMID： 25447857 DOI： 10.1007/978-1-4939-2205-5_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745