Literature DB >> 25446907

Characterisation of the first ongoing outbreak due to KPC-3-producing Klebsiella pneumoniae (ST512) in Spain.

Lorena López-Cerero1, Pilar Egea2, Irene Gracia-Ahufinger3, Marcelino González-Padilla3, Fernando Rodríguez-López4, Jesús Rodríguez-Baño2, Alvaro Pascual5.   

Abstract

The aim of this study was to characterise carbapenem-resistant Klebsiella pneumoniae isolates that caused an outbreak in a hospital in the south of Spain, originating from a patient transferred in 2012 from Italy. Forty-four K. pneumoniae isolates, recovered from 28 patients, were screened by PCR for extended-spectrum β-lactamase (ESBL) and carbapenemase genes and the products were further sequenced. Plasmids were transferred by electroporation and were classified using PCR-based Inc/rep typing and IncF subtyping. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to determine the genetic relatedness of the isolates. All isolates yielded positive modified Hodge test results, harboured bla(SHV-11), bla(TEM-1) and bla(KPC-3) genes, showed an identical PFGE pattern, and were assigned to clone sequence type 512 (ST512). The bla(KPC-3) gene was located on a 140-kb K2:A-:B-plasmid. In conclusion, the successful K. pneumoniae ST512 clone caused a major outbreak in Spain from an imported case and is the first description of an outbreak in this country due to the KPC-3-producing K. pneumoniae ST512 clone.
Copyright © 2014 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

Entities:  

Keywords:  KPC-3; Outbreak; ST512

Mesh：

Substances：

Year:  2014        PMID： 25446907     DOI： 10.1016/j.ijantimicag.2014.08.006

Source DB:  PubMed          Journal:  Int J Antimicrob Agents        ISSN： 0924-8579            Impact factor:   5.283


  14 in total

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Journal:  J Antimicrob Chemother       Date:  2016-08-15       Impact factor: 5.790

Review 2.  Treatment of Infections Caused by Extended-Spectrum-Beta-Lactamase-, AmpC-, and Carbapenemase-Producing Enterobacteriaceae.

Authors:  Jesús Rodríguez-Baño; Belén Gutiérrez-Gutiérrez; Isabel Machuca; Alvaro Pascual
Journal:  Clin Microbiol Rev       Date:  2018-02-14       Impact factor: 26.132

3.  Mortality Associated with Bacteremia Due to Colistin-Resistant Klebsiella pneumoniae with High-Level Meropenem Resistance: Importance of Combination Therapy without Colistin and Carbapenems.

Authors:  Isabel Machuca; Belén Gutiérrez-Gutiérrez; Irene Gracia-Ahufinger; Francisco Rivera Espinar; Ángela Cano; Julia Guzmán-Puche; Elena Pérez-Nadales; Clara Natera; Marina Rodríguez; Rafael León; Juan J Castón; Fernando Rodríguez-López; Jesús Rodríguez-Baño; Julián Torre-Cisneros
Journal:  Antimicrob Agents Chemother       Date:  2017-07-25       Impact factor: 5.191

Review 4.  Carbapenemase-Producing Klebsiella pneumoniae, a Key Pathogen Set for Global Nosocomial Dominance.

Authors:  Johann D D Pitout; Patrice Nordmann; Laurent Poirel
Journal:  Antimicrob Agents Chemother       Date:  2015-07-13       Impact factor: 5.191

5.  Impact of KPC Production and High-Level Meropenem Resistance on All-Cause Mortality of Ventilator-Associated Pneumonia in Association with Klebsiella pneumoniae.

Authors:  Francisco Rivera-Espinar; Isabel Machuca; Rocio Tejero; Jorge Rodríguez; Ana Mula; Eduardo Marfil; Ángela Cano; Belén Gutiérrez-Gutiérrez; Marina Rodríguez; Juan Carlos Pozo; Carmen De la Fuente; Jesús Rodriguez-Baño; Luis Martínez-Martínez; Rafael León; Julian Torre-Cisneros
Journal:  Antimicrob Agents Chemother       Date:  2020-05-21       Impact factor: 5.191

6.  Tracking KPC-3-producing ST-258 Klebsiella pneumoniae outbreak in a third-level hospital in Granada (Andalusia, Spain) by risk factors and molecular characteristics.

Authors:  Carmen Soria-Segarra; Pablo González-Bustos; Lorena López-Cerero; Felipe Fernández-Cuenca; María Dolores Rojo-Martín; María Amelia Fernández-Sierra; José Gutiérrez-Fernández
Journal:  Mol Biol Rep       Date:  2019-12-02       Impact factor: 2.316

7.  Epidemiology and biomolecular characterization of carbapenem-resistant klebsiella pneumoniae in an Italian hospital.

Authors:  M L Cristina; M Sartini; G Ottria; E Schinca; N Cenderello; M P Crisalli; P Fabbri; G Lo Pinto; D Usiglio; A M Spagnolo
Journal:  J Prev Med Hyg       Date:  2016-09

8.  In vitro Activity of Pentamidine Alone and in Combination With Aminoglycosides, Tigecycline, Rifampicin, and Doripenem Against Clinical Strains of Carbapenemase-Producing and/or Colistin-Resistant Enterobacteriaceae.

Authors:  Tania Cebrero-Cangueiro; Rocío Álvarez-Marín; Gema Labrador-Herrera; Younes Smani; Elisa Cordero-Matía; Jerónimo Pachón; María Eugenia Pachón-Ibáñez
Journal:  Front Cell Infect Microbiol       Date:  2018-10-18       Impact factor: 5.293

Review 9.  Current status of ESKAPE microorganisms in Spain: Epidemiology and resistance phenotypes.

Authors:  J Sánchez-López; R Cantón
Journal:  Rev Esp Quimioter       Date:  2019-09       Impact factor: 1.553

10.  Efficacy of Colistin and Its Combination With Rifampin in Vitro and in Experimental Models of Infection Caused by Carbapenemase-Producing Clinical Isolates of Klebsiella pneumoniae.

Authors:  María E Pachón-Ibáñez; Gema Labrador-Herrera; Tania Cebrero-Cangueiro; Caridad Díaz; Younes Smani; José P Del Palacio; Jesús Rodríguez-Baño; Alvaro Pascual; Jerónimo Pachón; M Carmen Conejo
Journal:  Front Microbiol       Date:  2018-05-15       Impact factor: 5.640
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