Yang He1, Kai-Yu Wang2, Dan Xiao3, De-Fang Chen4, Lingyuan Huang1, Tianqiang Liu3, Jun Wang1, Yi Geng5, Er-Long Wang1, Qian Yang1. 1. Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China. 2. Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China. Electronic address: kywang@sicau.edu.cn. 3. Animal Health Research Institute of Tongwei Co., Ltd., Chengdu 610041, Sichuan, PR China. 4. Department of Aquaculture, College of Animal Science& Technology, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China. 5. Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China.
Abstract
PURPOSE: Tilapia is an important agricultural fish that has been plagued by Group B streptococcus (GBS) infections in recent years, some of them severe. It is well-known that surface immunogenicity protein (Sip) is an effective vaccine against GBS. EXPERIMENTAL DESIGN: Since Sip was not expressed in either E. coli BL21 or E. coli Rosetta, we removed the N-terminal signal peptide and LysM of the virus to produce purified truncated Sip (tSip(1)), which multiplied easily in an E. coli host. The antibody's ability to recognize and combine with GBS was determined by Western-blot and specific staining in vitro. The relative percentage of survival (RPS), antibody titers, bacterial recovery, and pathologic morphology were monitored in vivo to evaluate the immune effects. Freund's incomplete adjuvant (FIA) plus tSip and aluminum hydroxide gel (AH) plus tSip were also evaluated. RESULTS: It revealed that tSip mixed with FIA was an effective vaccine against GBS in tilapia, while AH is toxic to tilapia.
PURPOSE: Tilapia is an important agricultural fish that has been plagued by Group B streptococcus (GBS) infections in recent years, some of them severe. It is well-known that surface immunogenicity protein (Sip) is an effective vaccine against GBS. EXPERIMENTAL DESIGN: Since Sip was not expressed in either E. coli BL21 or E. coli Rosetta, we removed the N-terminal signal peptide and LysM of the virus to produce purified truncated Sip (tSip(1)), which multiplied easily in an E. coli host. The antibody's ability to recognize and combine with GBS was determined by Western-blot and specific staining in vitro. The relative percentage of survival (RPS), antibody titers, bacterial recovery, and pathologic morphology were monitored in vivo to evaluate the immune effects. Freund's incomplete adjuvant (FIA) plus tSip and aluminum hydroxide gel (AH) plus tSip were also evaluated. RESULTS: It revealed that tSip mixed with FIA was an effective vaccine against GBS in tilapia, while AH is toxic to tilapia.
Authors: Erlong Wang; Kaiyu Wang; Defang Chen; Jun Wang; Yang He; Bo Long; Lei Yang; Qian Yang; Yi Geng; Xiaoli Huang; Ping Ouyang; Weimin Lai Journal: Int J Mol Sci Date: 2015-04-30 Impact factor: 5.923