Yonggang Gao1, Kai Zhang2, Fengli Zhu1, Zhonglin Wu1, Xi Chu3, Xuan Zhang4, Ying Zhang4, Jianping Zhang4, Li Chu5. 1. Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, Hebei, China. 2. Department of Basic Theory of Traditional Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China. 3. The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China. 4. Department of Pharmacology, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China. 5. Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, Hebei, China; Department of Pharmacology, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China. Electronic address: chuli0614@126.com.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Salvia miltiorrhiza (SM, Danshen), a traditional Chinese herbal drug, has been widely used for hundreds of years to treat coronary artery disease. MATERIALS AND METHODS: We studied the effects of SM on the L-type Ca(2+) current (ICa-L) with whole-cell patch-clamp technique in rat ventricular myocytes, and its influence on Ca(2+) transient and contractility using video-based edge detection and dual excitation fluorescence photomultiplier systems as well. RESULTS: Exposure to SM solution caused a concentration- and voltage-dependent blockade of ICa-L, and the dose of SM solution (10g/l) decreased the maximal inhibitory effect of 35.2±1.2%. However, SM solution did not significantly change the current-voltage relationship or reversal potential of ICa-L, nor did it altered the activation and inactivation gating properties of cardiac Ca(2+) channels. Meanwhile, SM decreased the amplitude of myocyte shortening and the peak value of Ca(2+) transient with a significant decrease in the time to 90% of the baseline (Tr), but the time to 10% of the peak (Tp) was not dramatically prolonged. CONCLUSIONS: The results indicated that SM significantly inhibited L-type Ca(2+) channels, decreased [Ca(2+)]i and contractility in adult rat cardiac myocytes. These findings may be relevant to the cardioprotective efficacy of SM.
ETHNOPHARMACOLOGICAL RELEVANCE: Salvia miltiorrhiza (SM, Danshen), a traditional Chinese herbal drug, has been widely used for hundreds of years to treat coronary artery disease. MATERIALS AND METHODS: We studied the effects of SM on the L-type Ca(2+) current (ICa-L) with whole-cell patch-clamp technique in rat ventricular myocytes, and its influence on Ca(2+) transient and contractility using video-based edge detection and dual excitation fluorescence photomultiplier systems as well. RESULTS: Exposure to SM solution caused a concentration- and voltage-dependent blockade of ICa-L, and the dose of SM solution (10g/l) decreased the maximal inhibitory effect of 35.2±1.2%. However, SM solution did not significantly change the current-voltage relationship or reversal potential of ICa-L, nor did it altered the activation and inactivation gating properties of cardiac Ca(2+) channels. Meanwhile, SM decreased the amplitude of myocyte shortening and the peak value of Ca(2+) transient with a significant decrease in the time to 90% of the baseline (Tr), but the time to 10% of the peak (Tp) was not dramatically prolonged. CONCLUSIONS: The results indicated that SM significantly inhibited L-type Ca(2+) channels, decreased [Ca(2+)]i and contractility in adult rat cardiac myocytes. These findings may be relevant to the cardioprotective efficacy of SM.
Authors: Jia-Ying Qi; Ya-Kun Yang; Chuan Jiang; Yang Zhao; Yong-Chao Wu; Xue Han; Xuan Jing; Zhong-Lin Wu; Li Chu Journal: Front Cardiovasc Med Date: 2022-02-28