M Jessri1, A J Dalley2, C S Farah3. 1. The University of Queensland, UQ Centre for Clinical Research, Herston, QLD 4029, Australia; The University of Queensland, School of Dentistry, Brisbane, QLD 4000, Australia. 2. The University of Queensland, UQ Centre for Clinical Research, Herston, QLD 4029, Australia. 3. The University of Queensland, UQ Centre for Clinical Research, Herston, QLD 4029, Australia; The University of Queensland, School of Dentistry, Brisbane, QLD 4000, Australia. Electronic address: camile@oralmedpath.com.au.
Abstract
OBJECTIVES: This study explored the expression of DNA mismatch repair (MMR) proteins in a range of oral biopsies. We further evaluated the significance of MMR protein expression combined with basic demographic data in differentiating grades of oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC). STUDY DESIGN: Immunohistochemical expression of MutSα (hMLH1 and hPMS2) and MutLα (hMSH2 and hMSH6) were compared in 98 formalin-fixed paraffin embedded oral biopsies: 21 normal, 24 mild-dysplasia (MD), 8 moderate-to-severe-dysplasia (SD), and 45 OSCC. RESULTS: Expression of hMLH1, hPMS2, and hMSH2 was reduced in MD, SD, and OSCC compared with the normal. Reduced hMSH2 immunoreactivity discriminated poorly differentiated OSCC from well-differentiated OSCC. The diagnostic model correctly classified 71.4% of cases and revealed that hPMS2-negative biopsies were more likely to be cancerous (odds ratio [OR], 0.11; 95% confidence interval [CI], 0.000-0.813; P = .040). CONCLUSION: The results suggested a diagnostic role for MMR proteins in OED and OSCC.
OBJECTIVES: This study explored the expression of DNA mismatch repair (MMR) proteins in a range of oral biopsies. We further evaluated the significance of MMR protein expression combined with basic demographic data in differentiating grades of oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC). STUDY DESIGN: Immunohistochemical expression of MutSα (hMLH1 and hPMS2) and MutLα (hMSH2 and hMSH6) were compared in 98 formalin-fixed paraffin embedded oral biopsies: 21 normal, 24 mild-dysplasia (MD), 8 moderate-to-severe-dysplasia (SD), and 45 OSCC. RESULTS: Expression of hMLH1, hPMS2, and hMSH2 was reduced in MD, SD, and OSCC compared with the normal. Reduced hMSH2 immunoreactivity discriminated poorly differentiated OSCC from well-differentiated OSCC. The diagnostic model correctly classified 71.4% of cases and revealed that hPMS2-negative biopsies were more likely to be cancerous (odds ratio [OR], 0.11; 95% confidence interval [CI], 0.000-0.813; P = .040). CONCLUSION: The results suggested a diagnostic role for MMR proteins in OED and OSCC.
Authors: Osias Vieira de Oliveira Filho; Thinali Sousa Dantas; Isabelle Joyce de Lima Silva-Fernandes; Maria do Perpétuo Socorro Saldanha Cunha; Ana Paula Negreiros Nunes Alves; Mário Rogério Lima Mota; Paulo Goberlânio de Barros Silva; Fabrício Bitu Sousa Journal: Head Neck Pathol Date: 2021-01-27
Authors: R Bologna-Molina; V Pereira-Prado; C Sánchez-Romero; G Tapia-Repetto; S Soria; M Hernandez; R Gónzalez-Gónzalez; M Molina-Frechero; T Mikami Journal: Med Oral Patol Oral Cir Bucal Date: 2018-03-01