Xue Xiao1, Na-Na Ping1, Sen Li1, Lei Cao2, Yong-Xia Cao1. 1. Department of Pharmacology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China. 2. Department of Pharmacology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China. Electronic address: leicao@mail.xjtu.edu.cn.
Abstract
AIMS: The aim of the present study was to determine the optimal initial tension for the rat basilar artery when using wire myography. METHODS: Rat basilar arteries were mounted in myograph baths. A normalization procedure was performed. K(+)-rich (60mM) buffer solution-induced tension was measured in different initial tensions. RESULTS: The initial tension of the basilar artery increased from 0.47 to 2.68mN/mm. Contractile tension was also elevated along with the initial tension. When the initial tension reached 1.63mN/mm, K(+)-induced contractile tension of basilar artery achieved its maximum. Thereafter, contractile tension declined as initial tension increased. The duration of equilibration time did not affect K(+)-induced contractile tension. CONCLUSION: The optimal initial tension is 1.63±0.01mN/mm in rat basilar arteries when using wire myography.
AIMS: The aim of the present study was to determine the optimal initial tension for the rat basilar artery when using wire myography. METHODS:Rat basilar arteries were mounted in myograph baths. A normalization procedure was performed. K(+)-rich (60mM) buffer solution-induced tension was measured in different initial tensions. RESULTS: The initial tension of the basilar artery increased from 0.47 to 2.68mN/mm. Contractile tension was also elevated along with the initial tension. When the initial tension reached 1.63mN/mm, K(+)-induced contractile tension of basilar artery achieved its maximum. Thereafter, contractile tension declined as initial tension increased. The duration of equilibration time did not affect K(+)-induced contractile tension. CONCLUSION: The optimal initial tension is 1.63±0.01mN/mm in rat basilar arteries when using wire myography.
Authors: Camilla F Wenceslau; Cameron G McCarthy; Scott Earley; Sarah K England; Jessica A Filosa; Styliani Goulopoulou; David D Gutterman; Brant E Isakson; Nancy L Kanagy; Luis A Martinez-Lemus; Swapnil K Sonkusare; Pratish Thakore; Aaron J Trask; Stephanie W Watts; R Clinton Webb Journal: Am J Physiol Heart Circ Physiol Date: 2021-05-14 Impact factor: 5.125