Raffaele Serra1, Raffaele Grande2, Rossella Montemurro2, Lucia Butrico2, Francesco Giuseppe Caliò3, Diego Mastrangelo4, Edoardo Scarcello5, Luca Gallelli6, Gianluca Buffone2, Stefano de Franciscis7. 1. Interuniversity Center of Phlebolymphology (CIFL), International Research and Educational Program in Clinical and Experimental Biotechnology, University Magna Graecia of Catanzaro, Catanzaro, Italy; Department of Medical and Surgical Sciences, University Magna Graecia of Catanzaro, Catanzaro, Italy. Electronic address: rserra@unicz.it. 2. Interuniversity Center of Phlebolymphology (CIFL), International Research and Educational Program in Clinical and Experimental Biotechnology, University Magna Graecia of Catanzaro, Catanzaro, Italy. 3. Division of Vascular Surgery, S. Anna Hospital, Catanzaro, Italy. 4. Unit of Vascular and Endovascular Surgery, GE.P.O.S. Hospital, Telese Terme, Italy. 5. Unit of Vascular and Endovascular Surgery, Regional Hospital of Cosenza, Cosenza, Italy. 6. Department of Health Sciences, University Magna Graecia of Catanzaro, Catanzaro, Italy. 7. Interuniversity Center of Phlebolymphology (CIFL), International Research and Educational Program in Clinical and Experimental Biotechnology, University Magna Graecia of Catanzaro, Catanzaro, Italy; Department of Medical and Surgical Sciences, University Magna Graecia of Catanzaro, Catanzaro, Italy.
Abstract
INTRODUCTION: An association between arterial aneurysms and matrix metalloproteinases (MMPs) has been described previously. MMPs regulate extracellular structural proteins and tissue remodeling. Neutrophil gelatinase-associated lipocalin (NGAL) is involved in the regulation of MMP activity. The aim of this work was to study the relationship between the levels of MMPs and NGAL and arterial aneurysms. METHODS: In a multicenter, open-label, parallel groups, prospective study, patients with aneurysmal disease were divided into 2 groups: Group I (with ruptured aneurysm) and group II (with nonruptured aneurysm). Healthy volunteer patients were also enrolled and represented the control group (group III). RESULTS: We enrolled 307 patients (107 in group I and 200 in group II) with arterial aneurysm: 49 popliteal, 31 common femoral, 2 superficial femoral, 29 common iliac artery, 3 common carotid, and 193 abdominal aorta. Finally, 11 healthy volunteer patients (9 males and 2 females; age range, 40-70 years; median 56) were enrolled in group III. Enzyme-linked immunosorbent assay and Western blot analysis revealed greater levels of immunoreactive MMP-9 and NGAL in all patients with ruptured aneurysms, both central and peripheral aneurysms, and in the aneurismal vessels. CONCLUSION: These results provide potentially important insights to the understanding of the natural history of arterial aneurysms. MMPs and NGAL play a role in development of arterial aneurysms and may represent molecular markers for the prevention of aneurysmal rupture.
INTRODUCTION: An association between arterial aneurysms and matrix metalloproteinases (MMPs) has been described previously. MMPs regulate extracellular structural proteins and tissue remodeling. Neutrophil gelatinase-associated lipocalin (NGAL) is involved in the regulation of MMP activity. The aim of this work was to study the relationship between the levels of MMPs and NGAL and arterial aneurysms. METHODS: In a multicenter, open-label, parallel groups, prospective study, patients with aneurysmal disease were divided into 2 groups: Group I (with ruptured aneurysm) and group II (with nonruptured aneurysm). Healthy volunteer patients were also enrolled and represented the control group (group III). RESULTS: We enrolled 307 patients (107 in group I and 200 in group II) with arterial aneurysm: 49 popliteal, 31 common femoral, 2 superficial femoral, 29 common iliac artery, 3 common carotid, and 193 abdominal aorta. Finally, 11 healthy volunteer patients (9 males and 2 females; age range, 40-70 years; median 56) were enrolled in group III. Enzyme-linked immunosorbent assay and Western blot analysis revealed greater levels of immunoreactive MMP-9 and NGAL in all patients with ruptured aneurysms, both central and peripheral aneurysms, and in the aneurismal vessels. CONCLUSION: These results provide potentially important insights to the understanding of the natural history of arterial aneurysms. MMPs and NGAL play a role in development of arterial aneurysms and may represent molecular markers for the prevention of aneurysmal rupture.
Authors: Ayman A Saeyeldin; Camilo A Velasquez; Syed Usman B Mahmood; Adam J Brownstein; Mohammad A Zafar; Bulat A Ziganshin; John A Elefteriades Journal: Gen Thorac Cardiovasc Surg Date: 2017-12-04
Authors: Stefano de Franciscis; Giovanni De Caridi; Mafalda Massara; Francesco Spinelli; Luca Gallelli; Gianluca Buffone; Francesco G Caliò; Lucia Butrico; Raffaele Grande; Raffaele Serra Journal: Int Wound J Date: 2014-12-03 Impact factor: 3.315
Authors: Giovanni De Caridi; Mafalda Massara; Francesco Spinelli; Antonio David; Sebastiano Gangemi; Francesco Fugetto; Raffaele Grande; Lucia Butrico; Roberta Stefanelli; Manuela Colosimo; Stefano de Franciscis; Raffaele Serra Journal: Int Wound J Date: 2015-05-27 Impact factor: 3.315
Authors: Raffaele Serra; Luca Gallelli; Lucia Butrico; Gianluca Buffone; Francesco G Caliò; Giovanni De Caridi; Mafalda Massara; Andrea Barbetta; Bruno Amato; Miriam Labonia; Selena Mimmi; Enrico Iaccino; Stefano de Franciscis Journal: Int Wound J Date: 2016-03-15 Impact factor: 3.315
Authors: Stefano de Franciscis; Luca Gallelli; Bruno Amato; Lucia Butrico; Alessio Rossi; Gianluca Buffone; Francesco G Caliò; Giovanni De Caridi; Raffaele Grande; Raffaele Serra Journal: Int Wound J Date: 2015-09-24 Impact factor: 3.315
Authors: Raffaele Grande; Gioia Brachini; Antonio V Sterpetti; Valeria Borrelli; Raffaele Serra; Francesco Pugliese; Giuseppe D'Ermo; Elvira Tartaglia; Paolo Rubino; Andrea Mingoli; Paolo Sapienza Journal: Int Wound J Date: 2019-10-27 Impact factor: 3.315