Jakov Meštrović1, Irena Drmić-Hofman2, Zenon Pogorelić3, Katarina Vilović4, Daniela Šupe-Domić5, Ana Šešelja-Perišin6, Vesna Capkun7. 1. Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Split, Croatia. 2. Department of Medical Chemistry and Biochemistry, University of Split School of Medicine, Split, Croatia; Department of Pathology, Forensic Medicine and Cytology, University of Split School of Medicine, Split, Croatia. 3. Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Split, Croatia. Electronic address: zpogorelic@gmail.com. 4. Department of Pathology, Forensic Medicine and Cytology, Split University Hospital Centre and Split University School of Medicine, Split, Croatia. 5. Department of Medical Laboratory Diagnosis, Split University Hospital Centre, Split, Croatia. 6. Department of Pharmacology, University of Split School of Medicine, Split, Croatia. 7. Department of Nuclear Medicine, Split University Hospital Centre and Split University School of Medicine, Split, Croatia.
Abstract
OBJECTIVE: To investigate the effect of nifedipine on testicular torsion-detorsion injury. MATERIALS AND METHODS: Twenty-four adult male Sprague-Dawley rats were randomly divided into 3 groups, each containing 8 rats. Rats in the control group underwent a sham operation of the left testis. In the torsion-detorsion (T/D) group, the left testis was twisted at 720° for 3 hours. After 3 hours of reperfusion, at the end of the experiment, the testes were removed. Rats in the treatment group received the same surgical procedure as the T/D group, but nifedipine was administered intraperitoneally (100 μg/kg) 30 minutes before the time of detorsion. RESULTS: Unilateral testicular torsion-detorsion caused a significant increase in the malondialdehyde level and apoptosis and caused significant decreases in superoxide dismutase and glutathione peroxidase activities in ipsilateral testes. The rats treated with nifedipine had a significant decrease in malondialdehyde level and apoptosis and had significant increases in superoxide dismutase and glutathione peroxidase activities in ipsilateral testes compared with those of the T/D group. CONCLUSION: These results suggest that biochemical and histological torsion-detorsion injury occurs in the ipsilateral testes after a 3-hour torsion and 3-hour detorsion and that administration of nifedipine before detorsion prevents ischemia/reperfusion cellular damage in the testicular tissue.
OBJECTIVE: To investigate the effect of nifedipine on testicular torsion-detorsion injury. MATERIALS AND METHODS: Twenty-four adult male Sprague-Dawley rats were randomly divided into 3 groups, each containing 8 rats. Rats in the control group underwent a sham operation of the left testis. In the torsion-detorsion (T/D) group, the left testis was twisted at 720° for 3 hours. After 3 hours of reperfusion, at the end of the experiment, the testes were removed. Rats in the treatment group received the same surgical procedure as the T/D group, but nifedipine was administered intraperitoneally (100 μg/kg) 30 minutes before the time of detorsion. RESULTS: Unilateral testicular torsion-detorsion caused a significant increase in the malondialdehyde level and apoptosis and caused significant decreases in superoxide dismutase and glutathione peroxidase activities in ipsilateral testes. The rats treated with nifedipine had a significant decrease in malondialdehyde level and apoptosis and had significant increases in superoxide dismutase and glutathione peroxidase activities in ipsilateral testes compared with those of the T/D group. CONCLUSION: These results suggest that biochemical and histological torsion-detorsion injury occurs in the ipsilateral testes after a 3-hour torsion and 3-hour detorsion and that administration of nifedipine before detorsion prevents ischemia/reperfusion cellular damage in the testicular tissue.
Authors: Doaa I Mohamed; Doaa A Abou-Bakr; Samar F Ezzat; Hanaa F Abd El-Kareem; Hebatallah H Abo Nahas; Hosam A Saad; Amir E Mehana; Essa M Saied Journal: Pharmaceuticals (Basel) Date: 2021-11-25