Neil G Venter1, Andréa Monte-Alto-Costa2, Ruy G Marques3. 1. Department of Surgery, Rio de Janeiro State University, Boulevard Vinte e Oito de Setembro, 77, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil. Electronic address: neilventer@terra.com.br. 2. Department of Histology and Embryology, Rio de Janeiro State University, Av. Marechal Rondon, 381/HLA, São Francisco Xavier, Rio de Janeiro, RJ 20950-003, Brazil. Electronic address: amacosta@uerj.br. 3. Department of Surgery, Rio de Janeiro State University, Boulevard Vinte e Oito de Setembro, 77, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil. Electronic address: ruymarques@globo.com.
Abstract
BACKGROUND: Burns are common and recurrent events treated by physicians on a daily basis at most emergency rooms around the world. There is a constant need to understand the physiopathology of burns, so as to minimize their devastating results. The objective of the present report is to describe a burn apparatus in association with an innovative method of animal fixation, as to produce burns of varying sizes and depths. METHODS: Rats were subjected to burns of 60 °C, 70 °C, and 80 °C for 10 s and after 3 days half of the rats in each group were killed and the resulting lesions were analyzed using histological techniques. In the other half of the rats the wound was measured weakly until complete re-epithelialization. RESULTS: All burns were easily visible and the histological feature for the 60 °C burn was a superficial second-degree burn (28% of the dermis), for 70 °C we observed a deep second-degree burn (72% of the dermis), and in the 80 °C group, a third degree-burn was present (100% of the dermis). CONCLUSIONS: This is a safe, reliable, easy to construct and use model that has the ability to produce a regular and uniform reproducible burn due to precise temperature control associated with standardized animal positioning.
BACKGROUND: Burns are common and recurrent events treated by physicians on a daily basis at most emergency rooms around the world. There is a constant need to understand the physiopathology of burns, so as to minimize their devastating results. The objective of the present report is to describe a burn apparatus in association with an innovative method of animal fixation, as to produce burns of varying sizes and depths. METHODS:Rats were subjected to burns of 60 °C, 70 °C, and 80 °C for 10 s and after 3 days half of the rats in each group were killed and the resulting lesions were analyzed using histological techniques. In the other half of the rats the wound was measured weakly until complete re-epithelialization. RESULTS: All burns were easily visible and the histological feature for the 60 °C burn was a superficial second-degree burn (28% of the dermis), for 70 °C we observed a deep second-degree burn (72% of the dermis), and in the 80 °C group, a third degree-burn was present (100% of the dermis). CONCLUSIONS: This is a safe, reliable, easy to construct and use model that has the ability to produce a regular and uniform reproducible burn due to precise temperature control associated with standardized animal positioning.
Authors: Vlad Porumb; Alexandru Florentin Trandabăț; Cristina Terinte; Irina Draga Căruntu; Elena Porumb-Andrese; Mihail Gabriel Dimofte; Dragoş Pieptu Journal: Biomed Res Int Date: 2017-10-12 Impact factor: 3.411