BACKGROUND: Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor with a dismal prognosis. Despite intensive study on tumor biology, the underlying mechanisms of the unlimited proliferation and progressive local invasion are still poorly understood, and no effective treatment has been developed for GBM patients. AIMS: We determine the role of TRPM7 channels in the growth, migration, and infiltration of malignant glioma cells. METHODS: Using a combination of RT-PCR, Western blot, and patch-clamp techniques, we demonstrated the expression of functional TRPM7 channels of A172 cells, a human glioma cell line, as well as in human glioma tissues. Furthermore, we evaluated the role of TRPM7 in growth, migration, and infiltration of A172 cells with MTT and transwell migration and invasion assays. RESULTS: We showed the expression of functional TRPM7 channels in both A172 cells and human glioma tissues. Suppression of TRPM7 expression with TRPM7-siRNA dramatically reduced the proliferation, migration, and invasion of A172 cells. Pharmacological inhibition of TRPM7 channel with 2-aminoethoxydiphenyl borate (2-APB) showed a similar effect as TRPM7-siRNA. CONCLUSION: We demonstrate that human glioma cells express functional TRPM7 channel and that activation of this channel plays an important role in the proliferation, migration, and invasion of malignant glioma cells. TRPM7 channel may represent a novel and promising target for therapeutic intervention of malignant glioma.
BACKGROUND:Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor with a dismal prognosis. Despite intensive study on tumor biology, the underlying mechanisms of the unlimited proliferation and progressive local invasion are still poorly understood, and no effective treatment has been developed for GBMpatients. AIMS: We determine the role of TRPM7 channels in the growth, migration, and infiltration of malignant glioma cells. METHODS: Using a combination of RT-PCR, Western blot, and patch-clamp techniques, we demonstrated the expression of functional TRPM7 channels of A172 cells, a humanglioma cell line, as well as in humanglioma tissues. Furthermore, we evaluated the role of TRPM7 in growth, migration, and infiltration of A172 cells with MTT and transwell migration and invasion assays. RESULTS: We showed the expression of functional TRPM7 channels in both A172 cells and humanglioma tissues. Suppression of TRPM7 expression with TRPM7-siRNA dramatically reduced the proliferation, migration, and invasion of A172 cells. Pharmacological inhibition of TRPM7 channel with 2-aminoethoxydiphenyl borate (2-APB) showed a similar effect as TRPM7-siRNA. CONCLUSION: We demonstrate that humanglioma cells express functional TRPM7 channel and that activation of this channel plays an important role in the proliferation, migration, and invasion of malignant glioma cells. TRPM7 channel may represent a novel and promising target for therapeutic intervention of malignant glioma.
Authors: Carsten Schmitz; Anne-Laure Perraud; Catherine O Johnson; Kazunori Inabe; Megan K Smith; Reinhold Penner; Tomohiro Kurosaki; Andrea Fleig; Andrew M Scharenberg Journal: Cell Date: 2003-07-25 Impact factor: 41.582
Authors: M J Nadler; M C Hermosura; K Inabe; A L Perraud; Q Zhu; A J Stokes; T Kurosaki; J P Kinet; R Penner; A M Scharenberg; A Fleig Journal: Nature Date: 2001-05-31 Impact factor: 49.962
Authors: G J Brewer; J Espinosa; M P McIlhaney; T P Pencek; J P Kesslak; C Cotman; J Viel; D C McManus Journal: J Neurosci Methods Date: 2001-05-30 Impact factor: 2.390
Authors: Mahealani K Monteilh-Zoller; Meredith C Hermosura; Monica J S Nadler; Andrew M Scharenberg; Reinhold Penner; Andrea Fleig Journal: J Gen Physiol Date: 2003-01 Impact factor: 4.086
Authors: Wen-Liang Chen; Andrew Barszczyk; Ekaterina Turlova; Marielle Deurloo; Baosong Liu; Burton B Yang; James T Rutka; Zhong-Ping Feng; Hong-Shuo Sun Journal: Oncotarget Date: 2015-06-30