Saleh Muhammad Rahim1, Ekhlass Muhi Taha2, Muneef Saeb Al-janabi3, Bushra Ismael Al-douri3, Kumar Das Simon4, Abd Gaffar Mazlan4. 1. Dept.of Biology, Faculty of Education, University of Tikrit, 34001Tikrit,Salah Aldeen, Iraq ; School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, D.E., Malaysia. 2. Dept. of Chemistry, Faculty of Science for Women, University of Baghdad, Baghdad, Iraq. 3. Dept.of Biology, Faculty of Education, University of Tikrit, 34001Tikrit,Salah Aldeen, Iraq. 4. School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, D.E., Malaysia.
Abstract
BACKGROUND: Cymbopogon citratus (Poaceae) a tropical perennial herb plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. This study aimed at evaluating the protective effects of C. citratus aqueous extract against liver injury induced by hydrogen peroxide (H2O2), in male rats. MATERIALS AND METHODS: Twenty-five rats were randomly divided into five different groups of five animals in each group; (1) Control. (2) Received H2O2 (0.5%) with drinking water. (3), and (4) received H2O2 and C. citratus (100 mg·kg(-1) b wt), vitamin C (250 mg·kg(-1) b wt) respectively. (5), was given C. citratus alone. The treatments were administered for 30 days. Blood samples were collected and serum was used for biochemical assay including liver enzymes activities, total protein, total bilirubin and malonaldehyde, glutathione in serum and liver homogenates. Liver was excised and routinely processed for histological examinations. RESULTS: C. citratus attenuated liver damage due to H2O2 administration as indicated by the significant reduction (p<0.05), in the elevated levels of ALT, AST, ALP, LDH, TB, and MDA in serum and liver homogenates; increase in TP and GSH levels in serum and liver homogenates; and improvement of liver histo-pathological changes. These effects of the extract were similar to that of vitamin C which used as antioxidant reference. CONCLUSION: C. citratus could effectively ameliorate H2O2-induced oxidative stress and prevent liver injury in male rats.
BACKGROUND:Cymbopogon citratus (Poaceae) a tropical perennial herb plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. This study aimed at evaluating the protective effects of C. citratus aqueous extract against liver injury induced by hydrogen peroxide (H2O2), in male rats. MATERIALS AND METHODS: Twenty-five rats were randomly divided into five different groups of five animals in each group; (1) Control. (2) Received H2O2 (0.5%) with drinking water. (3), and (4) received H2O2 and C. citratus (100 mg·kg(-1) b wt), vitamin C (250 mg·kg(-1) b wt) respectively. (5), was given C. citratus alone. The treatments were administered for 30 days. Blood samples were collected and serum was used for biochemical assay including liver enzymes activities, total protein, total bilirubin and malonaldehyde, glutathione in serum and liver homogenates. Liver was excised and routinely processed for histological examinations. RESULTS:C. citratusattenuated liver damage due to H2O2 administration as indicated by the significant reduction (p<0.05), in the elevated levels of ALT, AST, ALP, LDH, TB, and MDA in serum and liver homogenates; increase in TP and GSH levels in serum and liver homogenates; and improvement of liver histo-pathological changes. These effects of the extract were similar to that of vitamin C which used as antioxidant reference. CONCLUSION:C. citratus could effectively ameliorate H2O2-induced oxidative stress and prevent liver injury in male rats.
Authors: Artur Figueirinha; Maria Teresa Cruz; Vera Francisco; M Celeste Lopes; Maria Teresa Batista Journal: J Med Food Date: 2010-06 Impact factor: 2.786
Authors: Mai M Al-Oqail; Nida N Farshori; Ebtesam S Al-Sheddi; Shaza M Al-Massarani; Maqsood A Siddiqui; Abdulaziz A Al-Khedhairy Journal: Mol Biol Rep Date: 2020-03-16 Impact factor: 2.316