Literature DB >> 2543395

Disruption of the Saccharomyces cerevisiae gene for NADPH-cytochrome P450 reductase causes increased sensitivity to ketoconazole.

T R Sutter1, J C Loper.   

Abstract

Strains of Saccharomyces cerevisiae deleted in the NADPH-cytochrome P450 reductase gene by transplacement are 200-fold more sensitive to ketoconazole, an inhibitor of the cytochrome P450 lanosterol 14 alpha-demethylase. Resistance is restored through complementation by the plasmid-borne wild type gene from either S. cerevisiae or Candida tropicalis. Neither Southern hybridization nor Western immunoblot techniques provided evidence for a second NADPH-cytochrome P450 reductase gene, suggesting that an alternate pathway may provide for the functions of this reductase in S. cerevisiae.

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Year:  1989        PMID： 2543395     DOI： 10.1016/s0006-291x(89)80139-1

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  14 in total

1.  Genome-wide expression patterns in Saccharomyces cerevisiae: comparison of drug treatments and genetic alterations affecting biosynthesis of ergosterol.

Authors:  G F Bammert; J M Fostel
Journal:  Antimicrob Agents Chemother       Date:  2000-05       Impact factor: 5.191

2.  Genetic analysis of Aspergillus niger mutants defective in benzoate-4-hydroxylase function.

Authors:  J G Boschloo; E Moonen; R F van Gorcom; H F Hermes; C J Bos
Journal:  Curr Genet       Date:  1991-04       Impact factor: 3.886

3.  Microsomal electron transfer in higher plants: cloning and heterologous expression of NADH-cytochrome b5 reductase from Arabidopsis.

Authors:  M Fukuchi-Mizutani; M Mizutani; Y Tanaka; T Kusumi; D Ohta
Journal:  Plant Physiol       Date:  1999-01       Impact factor: 8.340

Review 4.  Microbial cytochromes P450: biodiversity and biotechnology. Where do cytochromes P450 come from, what do they do and what can they do for us?

Authors:  Steven L Kelly; Diane E Kelly
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2013-01-06       Impact factor: 6.237

5.  Genetic analysis of azole resistance by transposon mutagenesis in Saccharomyces cerevisiae.

Authors:  D P Kontoyiannis
Journal:  Antimicrob Agents Chemother       Date:  1999-11       Impact factor: 5.191

6.  Cytochrome b₅ reductase-cytochrome b₅ as an active P450 redox enzyme system in Phanerochaete chrysosporium: atypical properties and in vivo evidence of electron transfer capability to CYP63A2.

Authors:  Khajamohiddin Syed; Chandramohan Kattamuri; Thomas B Thompson; Jagjit S Yadav
Journal:  Arch Biochem Biophys       Date:  2011-03-02       Impact factor: 4.013

7.  P450 redox enzymes in the white rot fungus Phanerochaete chrysosporium: gene transcription, heterologous expression, and activity analysis on the purified proteins.

Authors:  Venkataramanan Subramanian; Harshavardhan Doddapaneni; Khajamohiddin Syed; Jagjit S Yadav
Journal:  Curr Microbiol       Date:  2010-03-11       Impact factor: 2.188

8.  Identification of an NADH-cytochrome b(5) reductase gene from an arachidonic acid-producing fungus, Mortierella alpina 1S-4, by sequencing of the encoding cDNA and heterologous expression in a fungus, Aspergillus oryzae.

Authors:  E Sakuradani; M Kobayashi; S Shimizu
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

9.  The sporulation-specific enzymes encoded by the DIT1 and DIT2 genes catalyze a two-step reaction leading to a soluble LL-dityrosine-containing precursor of the yeast spore wall.

Authors:  P Briza; M Eckerstorfer; M Breitenbach
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-10       Impact factor: 11.205

10.  Disruption of the Candida albicans CYB5 gene results in increased azole sensitivity.

Authors:  K M Rogers; C A Pierson; N T Culbertson; C Mo; A M Sturm; J Eckstein; R Barbuch; N D Lees; M Bard
Journal:  Antimicrob Agents Chemother       Date:  2004-09       Impact factor: 5.191
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