Literature DB >> 2543290

Purification and characterization of the NADP-dependent glutamate dehydrogenase from Bacillus fastidiosus.

H J Op den Camp1, K D Liem, P Meesters, J M Hermans, C Van der Drift.   

Abstract

Ammonia assimilation in Bacillus fastidiosus proceeds via the NADP-dependent glutamate dehydrogenase. The enzyme, purified to homogeneity, is composed of identical subunits with a molecular weight of about 48,000 dalton. Presumably the enzyme is a hexamer. The enzyme is specific for NADP(H). The pH optima for the amination and deamination reactions are 7.7 and 8.6, respectively. The temperature optimum is 60 degrees C. Furthermore, temperature stability and apparent Km values for substrates of both the amination and deamination reactions were determined. Several metabolites were tested for their effect on the enzyme activity. Only malate and fumarate showed some inhibitory effect.

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Year:  1989        PMID： 2543290     DOI： 10.1007/bf00398509

Source DB:  PubMed          Journal:  Antonie Van Leeuwenhoek        ISSN： 0003-6072            Impact factor:   2.271


  9 in total

1.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

2.  A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

Authors:  J J Sedmak; S E Grossberg
Journal:  Anal Biochem       Date:  1977-05-01       Impact factor: 3.365

3.  Purification of NADP-dependent glutamate dehydrogenase from Pseudomonas aeruginosa and immunochemical characterization of its in vivo inactivation.

Authors:  R A Smits; F R Pieper; C Van der Drift
Journal:  Biochim Biophys Acta       Date:  1984-09-07

4.  'Glutamine(amide):2-oxoglutarate amino transferase oxido-reductase (NADP); an enzyme involved in the synthesis of glutamate by some bacteria.

Authors:  J L Meers; D W Tempest; C M Brown
Journal:  J Gen Microbiol       Date:  1970-12

5.  Purification and properties of glutamate synthase and glutamate dehydrogenase from Bacillus megaterium.

Authors:  I A Hemmilä; P I Mäntsälä
Journal:  Biochem J       Date:  1978-07-01       Impact factor: 3.857

6.  Glutamate dehydrogenase from Bacillus subtilis PCI 219. I. Purification and properties.

Authors:  K Kimura; A Miyakawa; T Imai; T Sasakawa
Journal:  J Biochem       Date:  1977-02       Impact factor: 3.387

7.  Uric acid degradation by Bacillus fastidiosus strains.

Authors:  G P Bongaerts; G D Vogels
Journal:  J Bacteriol       Date:  1976-02       Impact factor: 3.490

8.  Characterization of a pyridine nucleotide-nonspecific glutamate dehydrogenase from Bacteroides thetaiotaomicron.

Authors:  T L Glass; P B Hylemon
Journal:  J Bacteriol       Date:  1980-03       Impact factor: 3.490

9.  Properties of glutamate dehydrogenase purified from Bacteroides fragilis.

Authors:  I Yamamoto; A Abe; M Ishimoto
Journal:  J Biochem       Date:  1987-06       Impact factor: 3.387
  9 in total

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