Understanding and engineering beneficial plant-microbe interactions: plant growth promotion in energy crops.

Plant production systems globally must be optimized to produce stable high yields from limited land under changing and variable climates. Demands for food, animal feed, and feedstocks for bioenergy and biorefining applications, are increasing with population growth, urbanization and affluence. Low-input, sustainable, alternatives to petrochemical-derived fertilizers and pesticides are required to reduce input costs and maintain or increase yields, with potential biological solutions having an important role to play. In contrast to crops that have been bred for food, many bioenergy crops are largely undomesticated, and so there is an opportunity to harness beneficial plant-microbe relationships which may have been inadvertently lost through intensive crop breeding. Plant-microbe interactions span a wide range of relationships in which one or both of the organisms may have a beneficial, neutral or negative effect on the other partner. A relatively small number of beneficial plant-microbe interactions are well understood and already exploited; however, others remain understudied and represent an untapped reservoir for optimizing plant production. There may be near-term applications for bacterial strains as microbial biopesticides and biofertilizers to increase biomass yield from energy crops grown on land unsuitable for food production. Longer term aims involve the design of synthetic genetic circuits within and between the host and microbes to optimize plant production. A highly exciting prospect is that endosymbionts comprise a unique resource of reduced complexity microbial genomes with adaptive traits of great interest for a wide variety of applications.

Introduction

To meet demand for sustainable alternatives to fossil fuels, dedicated energy crops that produce high annual biomass yields on low-quality land and without the need for fertilizer and pesticide inputs are being developed. Desirable energy crop traits include efficient low-cost establishment, rapid growth and high-biomass yields in the absence of chemical inputs, abiotic and biotic stress tolerance and perenniality (Lewandowski ). Primary candidates include the tall grasses, for example Miscanthus and switchgrass, and fast-growing trees such as Poplar and willow (Simmons ; Tuck ). In the near term, a number of crops domesticated for food and forage are also being grown specifically for bioenergy, notably sugar cane and Sorghum (Heaton ). In order to maximize net energy, outputs per unit of land novel approaches to boost biomass yields are required, including the manipulation of plant–microbe interactions.

Boosting crop yields has for several decades been the domain of the chemical industry. The green revolution, which arose during the 1940s and 1960s, included the development of nitrogen fertilizer derived from the Haber–Bosch process, phosphates and various other nutrients and pesticides (Tilman, 1998). Modern crops have largely been bred in conjunction with these economically and energetically costly chemicals, and therefore, have been selected to produce high yields in their presence, potentially at the expense of beneficial plant–microbe interactions hosted by their ancestors. Indeed, this may contribute to the reduction in competitive advantage many crops experience, to the point they are dependent on humans for their distribution. However, in an age of rapid population growth and climate change, alternative solutions are required to maintain and increase crop yields sustainably, without a concurrent increase in resource utilization (Tikhonovich and Provorov, 2011). These new approaches will require application of biological solutions, including the manipulation and exploitation of beneficial plant–microbe interactions. In contrast to crops that have been bred for food, the majority of dedicated perennial bioenergy crops are largely undomesticated and so there is an opportunity to harness relationships which may have been inadvertently lost through agronomic practice and intensive plant breeding (Finlay, 2008). To conserve the best land for food production, energy crops must be grown on marginal land, and must therefore tolerate a range of abiotic and biotic stresses (Jones ). Furthermore, energy crops are ideal for developing and evaluating novel technologies and applications as they are not consumed by humans. They will therefore provide important data about the safety of the use of bacteria to boost crop yields, which may then be applied more widely.

Overwhelmingly, research into plant–microbial interactions has focused on three categories of plant–microbe interactions: the ancient symbiosis between land plants and arbuscular mycorrhizae (AM, Smith and Smith, 2011), nitrogen fixation by rhizobia within the nodules of legume roots (Oldroyd ) and pathogenesis (Dodds and Rathjen, 2010; Kachroo and Robin, 2013; Wirthmueller ). These systems are now well characterized and provide insights into common and diverged signalling pathways involved in plant–microbe interactions. However, symbiosis is the norm rather than the exception, and so understanding plant–microbe interactions is of fundamental importance to gaining insights into plant evolution and adaptation (Hirsch, 2004). Plants are constantly interacting with a range of benign and parasitic organisms including bacteria, fungi and invertebrates in the soil. Complex relationships based on reciprocal signalling between diverse microbial consortia and plants abound both in the rhizosphere and within the plant itself (Badri ; Evangelisti ).

The spectrum of plant–microbe interactions is highly complex, comprising diverse microbial species, potentially acting as consortia (Hirsch, 2004). Apart from a few specialized examples, such as the legume–rhizobia interaction, monospecific interactions are considered to be the exception. These complex communities are very dynamic and may include opportunistic plant or human pathogens that are repressed under normal conditions (Berg ). Consortia may be governed by the presence of functional groups to maintain resilience rather than selection of specific microbial species and may involve tripartite interactions, for example between plant, fungi and bacteria (Bonfante and Anca, 2009; Dames and Ridsdale, 2012).

Developments in methodology are essential to this field of research. Historically only readily culturable species were studied, with different media and growth conditions required for different classes of microbes (Stewart, 2012; Vartoukian ). A number of endosymbionts such as mycorrhizae are not amenable to culture in isolation and must be grown in the presence of host tissue (Hildebrandt ). Methodological advances such as fluorescent tagging have been critical to the study of bacterial endophytes (Elbeltagy ), enabling clear visualization of small numbers of cells within the host, but remain out of reach for uncultured species. There is a growing interest in uncultured microbes, as these potentially represent ‘obligate endophytes’ which live their entire life cycle within the plant tissues. With the advent of next-generation sequencing, this fascinating group is gradually becoming accessible to study, and consequently, the body of data is accumulating (Bulgarelli ).

While plants and microbes have traditionally been studied and manipulated separately, understanding the interactions between the plant and its microbial symbionts requires a more holistic approach. Computational integration of different data types will be required to enable dissection of this complex and dynamic system. The aim of this review is firstly to summarize our current knowledge about the contribution of both plant and microbe to beneficial plant–microbe interactions in nonlegumes, and secondly to discuss opportunities and challenges ahead in the manipulation of plant–microbe interactions, in particular endophytic bacteria, to optimize production from biomass crops.

Symbiosis—living together

Relationships between plants and microbes comprise both fungal and bacterial interactions and can be categorized in various ways, primarily based on location and relationship to the plant, summarized in Figure1. The distinction between free-living soil bacteria, the rhizosphere population and endosymbionts of a plant host may be a true continuum, with microbes able to move between the soil, the root zone and the root, and definition influenced by both theory and methodology. By contrast, the nature of the interaction requires specialization on the part of the microbe, and there is a gradient from obligate pathogen, to opportunistic pathogen, to parasite/commensal, to facultative endosymbiont, to obligate endosymbiont, to plastid, and ultimately to organelle. Some microbes are generalists, for example able to exist as opportunistic pathogen, commensal and facultative endosymbionts, depending on the environmental circumstances; however, niche adaptation requires genomic specialization, limiting the fitness of a given organism to fulfil multiple roles. Adaptation to life within a plant, to the exclusion of the ability to exist in the competitive environment of the soil, is a specialization of great interest and relatively little study. Furthermore, plants have evolved in the presence of these complex microbial communities, yet ‘our knowledge of how this ‘symbiome’ influences host evolution, and development is woefully inadequate’ (Hirsch, 2004). In this context, we are especially interested in those microbes that have specialized as beneficial plant endosymbionts, the key mechanisms by which these interactions are maintained and how we might manipulate these relationships to optimize biomass production.

Figure 1

Plant–Microbe associations. Free-living bacteria in the soil (a), the rhizosphere population (b) and endosymbionts within the root (c) of a plant host may be a true continuum, with a subset of soil bacteria attracted to the rhizosphere (circles). A smaller number are able to enter the host and exist as endophytes (blue circles). The nature of the interaction with the plant requires specialization on the part of the microbe. Generalist microbes (squares and circles) tend to have larger genomes, enabling them to occupy different environmental niches and plant hosts, or to exist as facultative endosymbionts or opportunistic pathogens, depending on environmental circumstances. Niche adaptation requires genomic specialization, often via genome reduction (red triangles). The resulting LEANOMEs limit the fitness of a given organism to fulfil multiple roles, or even occupy different hosts, but offer potential tools for synthetic biology approaches to optimize plant–microbe interactions.

Quorum sensing and biofilm formation

Cell to cell communication between bacteria occurs via diffusible chemical signals and is collectively known as quorum sensing (QS, Greenberg, 1997). A number of bacterial genes are regulated by QS, including those involved in swarming, virulence and biofilm formation. Biofilms comprise multicellular assemblies of bacteria embedded in a complex extracellular matrix of exopolysaccharides and proteins. The formation of biofilms enables bacterial populations to adhere to environmental surfaces, including plant tissues, and is an intrinsic component of plant–microbe interactions (Ramey ). Transposon mutagenesis of plant-associated Bacillus amyloliquefaciens ssp. plantarum FZB42 identified genes required for bacterial swarming, biofilm formation, root colonization and plant growth promotion in axenic conditions (Budiharjo ). Bacteria within biofilms are phenotypically and physiologically distinct from free-living bacteria and demonstrate increased tolerance to antimicrobial compounds (Ramey ). The free-living diazotroph Azospilrillum brasilense stimulates root proliferation in wheat after forming dense biofilms on the root (Assmus ), and Bacillus subtilis mutants deficient in biofilm formation are unable to prevent infection of Arabidopsis by Pseudomonas syringae (Bais ). Mutagenesis of B. subtilis strain 6051 resulted in a mutant strain compromised in production of surfactin, a lipopeptide antimicrobial compound. Whereas B. subtilis strain 6051 forms biofilms and secretes surfactin to levels estimated to be lethal to P. syringae, the mutant was unable to form robust biofilms and was ineffective as a biocontrol (Bais ).

Plant–microbe signalling

Plant–microbe signalling in the soil occurs by means of chemical interaction, with both partners actively involved. Plants manipulate their interactions with the soil and soil microbes, at significant carbon cost, via rhizodeposition of diverse compounds from the roots. Rhizodeposition comprises root cap and border cells, mucilage, soluble root exudates, volatile organic carbon and the carbon lost to symbionts and through cell and tissue death (Jones ). Rhizodeposits, particularly the mucilage and root exudates, modulate the bacterial composition of the rhizosphere around the growing root (Dennis ). Different plants attract different populations of prokaryotes and eukaryotes to their rhizosphere (Turner ). Amino acids and carbohydrates released by the root may act as chemo-attractants, thereby accounting for the higher numbers of certain bacteria in the rhizosphere as compared to bulk soil (Bacilio-Jiménez ); however, a range of signalling molecules are required for the more subtle interactions. Differential gene expression in Pseudomonas aeruginosa strain PAO1 was observed in response to the root exudates of two different varieties of sugar beet, including previously uncharacterized genes associated with rhizosphere competition and bacterial colonization (Mark ). Reciprocal inoculation experiments of B. subtilis N11 (isolated from banana rhizosphere) and B. amyloliquefaciens SQR9 (isolated from cucumber rhizosphere) on banana and cucumber indicate more efficient colonization of the native host by bacteria from the rhizosphere of the two plants. Analysis of the root exudates of the two plants indicated components that induced both chemotaxis and biofilm formation in the native bacteria, but only one or the other in the non-native strain (Zhang ).

Plants inhibit neighbouring plants, herbivorous eukaryotes, and soil bacteria via chemical signalling from root exudates. A range of antimicrobial products are exuded in the root tip mucilage, providing a defensive zone around the meristematic and elongating root cells (Walker ). Certain legumes exude the compound canavanine, which is structurally similar to arginine, from their roots. If ingested and incorporated into nascent proteins in place of arginine, canavanine results in structurally aberrant proteins. While canavanine is toxic to many soil bacteria, certain rhizobial strains are able to detoxify canavanine and so are presumably advantaged relative to other bacteria in the rhizosphere of legume roots (Cai ). Furthermore, the plant transcriptome and proteome respond to bacterial quorum sensing signals (QSS) by pathogens and symbionts, with protein changes specific to the QSS structure and concentration (reviewed by Mathesius, 2009).

As demonstrated by these examples, QS and biofilm formation represent key targets for bacterial manipulation to optimize plant production.

Bacterial endophytes

In addition to soil and rhizosphere communities, large and diverse populations of microbes live within plants without causing signs of disease and are broadly termed endophytes. Bacterial endophytes reside within specific plants tissues, either inside the host cells or in the intracellular fluids, and have been isolated from all plant tissues (Rosenblueth and Martínez-Romero, 2006). They can be considered to sit at the benign end of the spectrum between mutualists and pathogens (Hirsch, 2004). These ancient relationships are not only fascinating from an evolutionary perspective, but are potentially of great value for sustainable plant production if these relationships can be understood and exploited.

The majority of endophytes are widely considered to represent a subset of soil bacteria which must colonize the plant without triggering the host defence response. Thus, they must exist in both free-living and endophytic states. In order to transition from the soil to the plant, the bacteria must first demonstrate rhizosphere competence and attachment to the root, followed by establishment in the host plant (Compant ). Once inside the plant, endophytes may be either extracellular or intracellular, surrounded by a host membrane. Both motility and secretion of various cellulases and pectinases are necessary attributes of bacteria transitioning from free-living to endophytic lifestyles (Reinhold-Hurek and Hurek, 2011). Endophytic bacteria do not create detrimental effects or cellular damage to the plant. Endophytic bacteria usually have lower population densities in the host plant tissues compared with pathogens, and this may be one method by which they evade the plant defences. There are however, reports of endophytic bacteria colonizing the host tissue internally, sometimes in high numbers, without damaging the host or eliciting symptoms of plant disease (Zinniel, 2002). Far from simply evading the attention of the plant, beneficial endophytes act in part by triggering the plant induced systemic resistance (ISR) towards pathogenic bacteria (reviewed by Kloepper and Ryu, 2006). In order to exploit beneficial bacteria to optimize biomass production, a far deeper understanding of both the individual components and their interactions is required.

Bacterial endophytes have been isolated from virtually all plants studied (Ryan ) including a number of potential bioenergy crops (summarized in Table1). This is almost certainly a considerable under-representation of the true diversity of endophytes within these species as many laboratories seek to isolate specific strains of interest rather than the full diversity present. Since the advent of next-generation sequencing, there has been a shift away from targeted isolation of small numbers of microbes towards large-scale projects aimed at sequencing the entire microbial population within an environmental niche. Metagenomic studies on energy crop species have not yet been reported, but in the same way that the human metagenome revealed the full extent of microbial associations with mammals (Zhao, 2010), diverse communities of endophytic bacteria have been identified in plant microbiome projects (Lundberg ; Sessitsch ). In terms of understanding biological function, a range of molecular tools are of use, including complete genome sequences (Table1), transcriptomics (Mark ; Shidore ; Straub ; Zuccaro ), proteomics (Lery ; Mathesius, 2009), and fluorescent tagging and localization studies (Compant ; Reinhold-Hurek and Hurek, 2011; Ryan ).

Table 1

Reported bacterial endophytes isolated from bioenergy crops

Bioenergy crop	Endophytic bacteria	References
Sorghum	Herbaspirillum seropedicae*	Baldani et al. (1986)*Pedrosa et al. (2011)
Pennisetum	Azospirillum brasilense, Gluconacetobacter diazotrophicus, Gluconacetobacter liquefaciens, Gluconacetobacter sacchari, Burkholderia silvatlantica, Klebsiella sp., Enterobacter cloacae and Enterobacter oryzae	Videira et al. (2012)
	Herbaspirillum-like Herbaspirillum frisingense sp., Herbaspirillum seropedicae	Kirchhof et al. (1997)Kirchhof et al. (2001)Olivares et al. (1996)
Sugarcane	Gluconacetobacter diazotrophicus (syn. Acetobacter diazotrophicus)*	Gillis (1989), Dong et al. (1994)*Berlatan et al. (2009)
	Burkholdeira, Pantoea, Pseudomonas, Microbacterium	Mendes et al. (2007)
	Citrobacter, Enterobacter, Pantoea, Klebsiella, Erwinia, Brevibacillus, Staphylococcus, Curtobacterium, Pseudomonas sp.	Magnani et al. (2010)
	Bacillus, Paenibacillus, Brevibacillus, Cohnella	Raton et al. (2012)
	Burkholdeira australis	Paungfoo-Lonhienne et al. (2014)
	Herbaspirillum seropedicae*, Herbaspirillum rubrisubalbicans	Olivares et al. (1996)*Pedrosa et al. (2011)
Miscanthus	Azospirillum-like, Azospirillum lipoferum-like, Herbaspirillum-like	Kirchhof et al. (1997)
	Azospirillum doereinerae sp. nov. GSF71	Eckert et al. (2001)
	Herbaspirillum frisingense sp.	Kirchhof et al. (2001) and Straub et al. (2013a)
	Clostridium spp.	Miyamoto et al. (2004)
Poplar	Methylobacterium populi, Pseudomonas sp.	Van Aken (2004), (summarised in van der Lelie, 2009)
	Enterobacter sp 638*	Taghavi et al. (2009)
	P. putida W619	*Taghavi et al. (2010)
	Serratia proteamaculans 568
	Stenotrophomonas maltophilia R551-3
	Burkholderia vietnamiensis	Doty et al. (2009)
	Pantoea sp.
	Pseudomonas graminis
	Rahnella sp. CDC 2987-79
	Enterobacter sp. YRL01
	Burkholderia sp. H801
	Acinetobacter calcoaceticus
Willow	Acinetobacter sp. PHD-4	Doty et al. (2009)
	Herbaspirillum
	Stenotrophomonas sp. LQX-11
	Sphingomonas yanoikuyae
	Pseudomonas sp. H9zhy
	Sphingomonas sp. ZnH-1
	Pseudomonas sp. H9zhy
	Sphingomonas yanoikuyae
	Sphingomonas sp. ZnH-1
	Pseudomonas sp. WAI-21
	Pantoea agglomerans*	*Gan et al. (2014)
	Staphylococcus haemolyticus*
	Pseudomonas sp.*
	Microbacterium oleivorans*
	Micrococcus luteus*
	Micrococcus luteus*
	Janthinobacterium lividum*
	Stenotrophomonas sp.*
	Delftia sp.*
	Micrococcus luteus*
	Sphingomonas sp.*
	Exiguobacterium sp.*
	Pseudomonas sp.*

Indicates a published genome sequence is available, and the corresponding reference.

Plant benefits and near-term exploitation

Bacteria that convey benefits to the plant are collectively termed plant growth promoting bacteria (PGPBs). PGPBs may either be rhizobacteria (PGPRs) or colonize plant roots to become endophytes, with a number of species moving between the two states (Compant ). There is a great potential for optimizing biomass production through the application of plant-associated bacteria, as evidenced by a 55% biomass increase in poplar cuttings 17 weeks after inoculation with Enterobacter sp. Strain 638 (Rogers ). PGPBs are diverse in their modes of action, including production of phytohormones, nitrogen acquisition, mobilization or enhanced uptake of soil minerals such as phosphorus, plant protection and control of pathogens. These benefits have not always been realized when applied to field situations, potentially due to insufficient rhizosphere or plant colonization (Compant ). Azospirillum, in particular, has been studied extensively both as a PGPR and as an endophyte and is used as a commercial inoculant to improve yields and/or reduce expensive fertilizer use (Baldani ; Bashan, 1998; Hungria ; Okon and Itzigsohn, 1995). However, the ability of endophytes to live within plant tissues represents a unique niche, increasing the potential for successful application to boost crop production, and presumably requiring genomic specialization (Hardoim ).

Phytohormone signalling

The roles of phytohormones in plant growth and development are fundamental, diverse and complex, combining both default developmental pathways and dynamic responses to the environment (reviewed recently by Durbak ). It is maybe unsurprising then that phytohormones are key components of plant–microbe interactions. Certain bacteria have the ability to produce phytohormones including indole-3-acetic acid (IAA, an auxin), gibberellin (GA) and cytokinin (CK) (Bottini ; Tsavkelova ). It has been theorized that phytohormones could be used as signalling molecules between bacteria and host, and the existing crosstalk between IAA and ethylene biosynthesis exploited as a means of communication (Spaepen ; Yuan ). Furthermore, bacteria can also influence and regulate phytohormone production by the plant.

Inoculation of Miscanthus seedlings with Herbaspirillum frisingense GSF30T, a temperate grass endophyte, promoted root and shoot growth; transcriptome analyses revealed regulation of jasmonate and ethylene signalling, indicating that the promotion of plant growth is modulated by phytohormone activity (Straub ). Of eleven different endophytic bacterial strains isolated from sweet potato, the cuttings inoculated with bacterial strains that produced indole acetic acid (IAA) and auxin produced roots first and grew more rapidly than uninoculated cuttings (Khan and Doty, 2009). Herbaspirillum frisingense GSF30T was demonstrated to produce IAA in culture (Rothballer ), and auxin was concluded to be the likely mechanism behind the increase in seedling growth of wheat plants inoculated with B. subtilis (Egorshina ). Azospirillum spp. are considered to increase plant growth primarily via root stimulation by auxin, with nitrogen fixation and other production of phytohormones playing lesser roles (Steenhoudt and Vanderleyden, 2000). These effects may well be applicable in field situations, for example Azopspirillum sp. strain B510, isolated from surface-sterilized stems of rice, significantly increased tiller number and yield of paddy field-grown rice plants following re-inoculation of seedlings (Isawa ) while three Pseudomonas strains enhanced growth and spike length in wheat in both laboratory and field conditions (Iqbal and Hasnain, 2013). These effects were attributed to phytohormone production rather than nitrogen fixation in both cases.

Ethylene plays an important role in both normal plant development and plant stress response. Ethylene synthesis is highly sensitive to environmental stimuli including light, temperature and other phytohormones, with production increased in response to a variety of biotic and abiotic stresses (Abeles ). Bacterial species with the ability to produce 1-aminocyclopropane-1-carboxylate (ACC) deaminase, for example Burkholderia spp., can degrade excess amounts of ACC (the direct precursor to ethylene) producing nitrogen and energy as a by-product, reducing the stress response and promoting growth (Onofre-Lemus ). Ethylene levels in the plant may be regulated by cleaving ACC or inhibiting its production; in either case, bacterial efficiency increases in close proximity to the plant cells in which ethylene biosynthesis occurs (Hardoim ). Bacteria with ACC deaminase activity frequently provide a range of other benefits and have been postulated to be major forerunners in the transition from chemicals to bacterial plant growth promotion in agricultural systems (Glick, 2014).

Nutrient acquisition

A number of bacterial endophytes have the ability to form symbioses with plants and to fix bio-available nitrogen within unspecialized tissues of the host plant, that is in the absence of nodulation as seen in the legume–rhizobia interaction. For example, Cyanobacteria can form associations with a range of plants from different clades including Gunnera, cycads, lichens and Azolla (Santi ) and form heterocysts; specialized structures creating a microaerophilic environment suitable for nitrogen fixation with the nitrogenase enzyme (Berman-Frank ).

Several diazotrophic bacterial species have been repeatedly identified as being associated with, or as bacterial endophytes of, Saccharum (sugar cane) in Brazil. These species include Gluconacetobacter diazotrophicus (formerly Acetobacter diazotrophicus), Azospirillum amazonense, A. brasilense, Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans (formerly Pseudomonas rubrisubalbicans, Kirchhof ; Monteiro ). Gluconacetobacter diazotrophicus can be endophytic in Saccharum and has been identified in electron microscopic studies using immunogold labelling techniques (James ). Both G. diazotrophicus and the mild plant pathogen Herbaspirillum spp. have been recorded in high numbers in sugar cane roots, stems and leaves (James and Olivares, 1998; Olivares ). Herbaspirillum seropedicae populations are reduced in bulk soil compared with plant-associated populations (Baldani ) suggesting the species is particularly suited to an endophytic life. Herbaspirillum rubrisubalbicans also has the ability to colonize sugar cane endophytically (James ). These species of diazotrophic bacteria are likely to be key contributors to the significant biological nitrogen fixation (BNF) that has been observed in field experiments using nitrogen balance and nitrogen isotope dilution techniques in Brazilian sugar cane (Baldani and Baldani, 2005; Boddey ; Döbereiner ; James, 2000).

Videira ), used semisolid media to culture bacteria from fresh tissue of two genotypes of Pennisetum purpureum to investigate possible nitrogen-fixing bacterial populations. The culturable diazotrophic bacterial population colonizing these plants varied from 102 to 106 bacteria/g fresh tissues. Diazotrophs identified belonging to the genera Gluconacetobacter, Azospirillum and Enterobacter colonized the plant tissues of both genotypes, similar to those found in Brazilian sugar cane and Miscanthus grown in Illinois (Davis ), indicating that these relationships are common to temperate and tropical systems.

In rice and maize, BNF contribution is similarly derived from a number of different species including members of Azospirillum, Azoarcus, Herbaspirillum, Bacillus, Enterobacter, Klebsiella and Pseudomonas (Boddey ; Hurek ; Kirchhof ; Monteiro ). In field experiments using wild rice, grain yields increased to the equivalent of using an additional nitrogen fertilizer application of 40 kg N/ha following inoculation with Herbaspirillum seropedicea (Baldani ; Pereira and Baldani, 1995). In another study, up to 30% of the total nitrogen accumulated in rice plants was derived from BNF, again demonstrating the potential gains to be made from bacterial associations (Malik ). Sixteen percent of plant nitrogen in field-grown Miscanthus plants was estimated to be derived from BNF, despite nonlimiting soil nitrogen (Keymer and Kent, 2013). However, A. diazotrophicus colonization of sugar cane is inhibited by high N-fertilization (Fuentes-Ramirez ), and exogenous nitrogen fertilizer has been demonstrated to reduce the number of diazotrophic endophytes cultured from sugar cane (Pariona-Llanos ). In Brazil, both rhizosphere and endophyte populations were demonstrated to be altered following the practice of adding vinasse, a concentrated by-product of the sugar extraction process rich in nutrients, back to soils to fertilize sugar cane (Leite ). These data indicate that diazotrophic relationships may not be retained by the plant in conditions where they are not conveying a benefit in terms of nitrogen availability. Interestingly, the ability to fix nitrogen of some diazotrophic Herbaspirillum strains has been documented in wild rice, but the results were not replicated in the same experiment with cultivated rice (Elbeltagy ; Kirchhof ). Genomic comparison of wild and cultivated rice should yield insights into the plant components required for successful plant–diazotroph interactions.

Finally, plants may obtain nitrogen from associated bacteria via active release of amino acids by diazotrophs. At least one, and up to four, amino acids were released from each of 22 strains of diazotrophic rhizobacteria isolated from sugar cane and grown on media free of combined-N. The excretion of amino acids was correlated with nitrogenase activity and included methionine and ornithine, both precursors of ethylene (de Oliveira ).

Plant protection and biocontrol

A range of essential microbial components, collectively termed microbe-associated molecular patterns (MAMPs) are recognized by plants and act as elicitors, triggering a generalized MAMP-triggered immunity (MTI). Although commonly described in the context of pathogenicity, MAMPs are conserved among nonpathogens including endophytes. MTI responses include the production of molecules such as reactive oxygen and nitrogen species, which act in signalling and as antimicrobial compounds (reviewed by Newman ). Induction of systemic plant resistance by either rhizosphere or endophytic bacteria is independent of salicylic acid accumulation and pathogen-related protein induction and is termed induced systematic resistance (ISR) to distinguish the response from systemic acquired resistance (SAR), which is triggered by pathogens (van Loon ; Pieterse, 1998). Pre-inoculation of Arabidopsis seedlings with two closely related strains of Streptomyces sp. protected the plants from disease symptoms following subsequent challenge by Erwinia caratovora while endophyte-free plants succumbed to rot within 5 days. Despite morphological and taxonomic similarity of the two strains, gene induction in Arabidopsis was specific to each of the two strains following inoculation, indicating ISR induction by one and SAR induction by the other. The host response is therefore fine-tuned to respond to different bacterial signals, further indicated through induction of ISR by bacterial exudate grown on a complex medium, and SAR induction by exudate of the same strain grown on minimal medium (Conn ). Furthermore, there is an evidence that pathogen infection itself triggers plant recruitment of beneficial rhizosphere bacteria. Infection of Arabidopsis by P. syringae induced a malic acid (MA) transporter, in turn led to an increase of MA in the rhizosphere. Bacillus subtilis, a beneficial rhizobacteria, numbers increased in response to MA, and stimulated ISR in the plant, thereby restricting the effect of the pathogen (Lakshmanan ).

The role of endophytes in eliciting plant defence in energy crops is not yet well studied, although G. diazotrophicus has been demonstrated to elicit a defence response against a plant pathogen in sugar cane (Arencibia ). Further analysis of the signalling that occurs following endophytic and pathogenic inoculation, such as the proteomic analysis conducted in sugar cane by Lery , will indicate whether similar or divergent mechanisms are involved in these crops in comparison to Arabidopsis. In this study, host genotype-specific responses were observed in the proteome of A. diazotrophicus, with one strain of sugar cane expressing proteins involved in root colonization, while the other elicited a strong defence, preventing a successful interaction. It is highly likely that the rhizosphere and endophytic populations will vary between crops grown at different geographies and that the resulting interactions will be largely specific to the plant and microbial strains as well as the environmental conditions. For energy crops, in particular, it will be of importance to design experiments to understand these interactions in controlled environments approximating those in the field.

An alternative mechanism of plant protection by rhizosphere and endophytic bacteria is the production of antimicrobial compounds. Rosmarinic acid, which demonstrated potent antimicrobial activity against a range of soil borne microorganisms, was induced in the exudates of sweet basil hairy root cultures following challenge by Pythium ultimum (Bais ). Endophytic actinobacteria, in particular, have been a rich source of novel bioactive compounds, including antibiotics, antifungals and antitumour compounds with great potential for exploitation (summarized in Qin ). In addition to the production of specific antimicrobial products, endophytic bacteria inhibit pathogenic QS, thereby inhibiting communication and biofilm formation, and hence virulence, without suppressing bacterial growth. Cell-free lysates from endophytic bacteria were demonstrated to degrade QS molecules and suppressed biofilm formation in P. aeruginosa PAO1 (Rajesh and Ravishankar, 2013). Thus, endophytic bacteria can protect the host against pathogens which have evolved resistance to the plant defences. This ‘quorum quenching’ is of great interest as an alternative antivirulence approach to tackling drug-resistant bacteria as it does not induce selective pressure for developing antibiotic resistance (Kusari ).

Abiotic stress tolerance

In an era of changing climates, there are obvious advantages to developing crops with tolerance to abiotic stresses such as drought and salinity. In the case of perennial energy crops, which are to be grown on marginal land, resilience to a wider range of stresses is essential. Such crops must overwinter annually and tolerate the climatic conditions over multiple seasons, perhaps for a decade or more. They must generate high-biomass yields on land unfit for food production; for example due to low or erratic rainfall, salinity or heavy metal pollution. While abiotic stress tolerance may be conferred by the plant genome, relationships with microbes can also provide improved tolerance to, or protection from, numerous abiotic stresses.

Burkholderia phytofirmans strain PsJN has a wide host spectrum, including wheat, maize and grapevine, and has been implicated in a range of beneficial abiotic stress tolerance. Photosynthetic rate, water-use efficiency and chlorophyll content of wheat inoculated with B. phytofirmans PsJN were improved with respect to controls under field conditions, ultimately resulting in increased grain yield (Naveed ). In maize shoot and root biomass, leaf area and photosynthetic efficiency was higher in droughted plants inoculated with both B. phytofirmans and Enterobacter sp. FD17 with respect to controls. Burkholderia phytofirmans offered more efficient protection against drought, indicating that physiological responses to endophyte inoculation are specific to the plant and microbial genotypes (Naveed ). Burkholderia phytofirmans PsJN induces resistance to grey mould and increases tolerance to low nonfreezing temperatures in grapevines. Following growth at 4 °C, more rapid and greater up-regulation of the plant stress-related gene transcripts and metabolites was observed in the plant in the presence of the bacteria, indicating a priming effect of the endophyte (Theocharis ). Burkholderia phytofirmans PsJN has been demonstrated to colonize and promote the growth of switchgrass under glasshouse conditions (Kim ), suggesting it may be an excellent candidate for bioenergy production enhancement.

A number of other endophytes have also been shown to confer tolerance against abiotic stresses to plants. Miscanthus was demonstrated to be more tolerant to salinity following inoculation with an anaerobic diazotroph Clostridium and a nondiazotrophic Enterobacter sp. Despite an initial slight retardation in growth with respect to uninoculated plants, inoculated plants were larger than the controls following continuous growth on 100 mM NaCl (Ye ). Gluconacetobacter diazotrophicus has a wide host range and is a common endophyte of sugar cane, where it tolerates high sucrose concentrations. Expression of levansucrase is required by the bacteria to hydrolyse sucrose to glucose and fructose for transport into the cell and subsequent metabolism. Disruption of the gene encoding levansucrase results in decreased levansucrase production, decreased tolerance to desiccation and decreased tolerance to NaCl, indicating that levansucrase may act as an osmoprotectant (Velázquez-Hernández ). Collectively, the physical and chemical bacteria-induced changes resulting in plant abiotic stress tolerance have been termed ‘induced systemic tolerance’ (IST) (Yang ).

Screening plants growing in extreme environments is a promising approach to isolating novel endophytes for application in energy crops to be grown under marginal conditions. Seventeen of 20 bacteria, predominantly Bacillus sp., isolated from halophyte and salt-tolerant plant species showed growth in culture on 7.5% NaCl, with all but two tolerating up to 10% NaCl (Arora ). The high frequency of halotolerance among the endophytes of plants growing in saline environments suggests that the plant may more readily recruit stress tolerance from a diverse bacterial population than develop innate tolerance via adaptation of the plant genome. If this is the case, this has broad implications for energy crop breeding and agronomy.

Phytoremediation

A potential dual benefit of an energy crop plantation is the possibility to use a biomass crop for phytoremediation of a contaminated site. In addition to harvesting a biomass crop for use as an industrial feedstock for fuel or renewable product production, an energy crop can be used to decontaminate land unsuitable for food production in order to bring it back into use. In pot experiments, endophytic Bacillus sp. SLS18 increased biomass of Sorghum grown in either manganese or cadmium. Similar effects were also observed in two dicotyledonous species, again indicating broad applicability in terms of host range (Luo ). Remediation of both organic compounds and toxic metals is possible, each dependent on effective plant–microbe interactions. Phytoremediation uses plants to clean up toxic soils, whereas the process phytoextraction uses species which uptake and accumulate trace element concentrating the pollutants in their tissues and out of the soil. Fast-growing high-biomass plants including Populus trichocarpa and Salix spp. are often used for phytoextraction, and the process is enhanced by inoculating the plants with bacterial endophytes. The plant provides a biological niche to support higher microbial densities of microbial populations or consortia able to successively transform contaminants. Contaminants may either be neutralized or stored in the plant and harvested, thereby remediating the soil. However, specialist applications or residual metal recovery may be required to prevent future recontamination from the biomass. Endophytes including Burkholderia cepacia have been demonstrated to both increase the efficiency of the remediation and also boost biomass production in the host (Weyens ,b).

Bioprospecting for endophytes in a range of hostile environments may be a route to developing energy crops tolerant to growth on contaminated soils. A high rate of cadmium tolerance was observed in endophytes isolated from the seed of tobacco plants grown with exposure to cadmium. When inoculated with these endophytes, tobacco plants accumulated increased biomass in both the presence and the absence of cadmium. Moreover, cadmium was accumulated to a greater concentration in endophyte inoculated plants (Mastretta ). Targeted screening of plants from extreme environments (saline, droughted, contaminated etc.) may yield a wealth of novel microbes with adaptive traits of interest for application in energy crops for growth on marginal land.

A summary of beneficial plant–microbe interactions and near-term applications is shown in Table2. The increasing number of patents in this area is indicative of the opportunities that these beneficial organisms present (Mei and Flinn, 2010). In practice, countless beneficial bacteria have yet to be isolated and identified, and of those that have, many confer multiple benefits to plants. Wide-spectrum benefits may be conferred to plants via associations with microbes, including both rhizosphere bacteria and endophytes. In addition to promoting plant growth via phytohormone production, PGPRs may further augment plant immunity and elicit both IST and nutrient uptake (Yang ). In a study of 102 bacteria associated with sugar cane roots, 74 were able to fix nitrogen and 77 were able to solubilize phosphate, all 102 produced IAA to at least some degree, 50 were positive for the production of the QS molecule N-acyl homoserine lactone (AHL), and 33 isolates were positive for all four tests. Twenty-seven isolates were further tested for salinity tolerance (Leite ). Harnessing these benefits to promote biomass crop growth will require a combination of detailed understanding of the component microbes and their interactions with plants, for example by mutagenesis (Rouws ), and also long-term field studies to determine the factors regulating microbial populations in the rhizosphere and soil. A near-term application is the development of strains to be used as seed coatings, biofertilizers and biopesticides.

Table 2

Summary of beneficial plant–microbe interactions and near-term applications

Activity	Application	Priority
Phytohormone production	Plant growth promotion	Develop synthetic consortia for use as yield boosting agents
Biological nitrogen fixation/phosphate soulbilization	Biofertilizer	Identify novel strains and elucidate host–microbe specificity mechanisms
Plant protection	Biocontrol	Screening of endophyte collections for antimicrobial properties and plant defence induction
Abiotic stress tolerance	Boost plant biomass on marginal land	Bioprospecting for endophytes of plants growing under extreme conditions, for example drought, cold and salinity
Phytoremediation	Remediation of contaminated land	Bioprospecting for endophytes of plants growing on a range of contaminated sites
Endophytic specialization	Novel pathways and reduced genomes for synthetic applications	Genome analysis of endophytic and closely related species and development of molecular parts and devices libraries

Synthetic bacterial populations

An alternative to identifying single strain isolates with a range of plant benefits is the potential for developing synthetic bacterial communities for application to crops. There is currently an increasing interest in generating synthetic consortia of two or more bacteria to address questions of community-level functions and properties. Previous reports, that competition between bacteria is common, were based on pairwise experiments of isolated strains (Foster and Bell, 2012); however, results of studies to date indicate that even paired interactions are complex in terms of function and stability. One strain may provide a metabolic effect with a negative, neutral or positive effect on the other; six motifs of microbial interactions are possible from a combination of two bacteria (Figure2), with this complexity rapidly increasing to 729 interaction states for a community of three strains, and 531 441 interaction states for a community of four strains (Großkopf and Soyer, 2014). In reality, even simple microbial communities sampled from the natural environment contain far higher numbers of individuals, with seeding of new strains possible at all times. Environmental conditions such as pH, temperature and nutrient availability will affect growth rates of the individuals (Goldfarb ), all of which change over time with bacterial growth. There may be a high or a low rate of seeding in different populations, with certain systems, such as anaerobic digesters, providing a relatively consistent environment in which a bacterial community may stabilize (Werner ). In comparison to the dynamic environment of the soil, the internal tissues of a plant are likely to provide a relative stable environment for a population of bacteria adapted to endophytic life. The challenge then is not to attempt to control this diversity at a species level, but to develop consortia with resilient functionality in terms of plant growth promotion.

Figure 2

The possible six motifs of microbial interactions between two bacterial strains (developed from Großkopf and Soyer, 2014).

Reduced/specialized microbial genomes

Although much attention has been paid to gain of function mutations, loss of function mutation can occur at high frequency and plays an important role in adaptation. Under selection, substantial adaptation to new environments, via altered metabolism, can be achieved through loss of function mutations (Hottes ). Bacteria that have evolved an obligate endosymbiotic relationship with their host are known to have undergone genome reduction during host adaptation stages compared with free living and often pathogenic-related species (Figure3). Statistical analyses confirm that among the γ-proteobacteria genome size is inversely correlated to the intracellular stage of host adaptation (Toft and Andersson, 2010). The genome of H. seropedicae SmR1, a specialized endophyte of tropical grasses, is composed of a circular chromosome of just over 5.5 Mbp, whereas the genome of the pathogen H. rubrisubalbicans M1, causal agent of mottled stripe disease and red stripe disease, was estimated to be over 50 Mbp. (Monteiro ; Pedrosa, 2011). In addition to differences between these species in a range of molecular factors potentially involved in colonization, almost 40% of the suppressive subtractive hybridization library of H. rubrisubalbicans M1 contained mobile elements [insertional sequences (IS)] compared with zero IS being identified in H. seropedicae (Monteiro ). These mobile elements are known to exert plasticity on the bacterial genome and facilitate activation or inactivation of genes resulting in altering the metabolic network and conferring an evolutionary selective advantage in highly variable environments. The absence of mobile elements and the small genome size of H. seropedicae SmR1, seemingly reflect its specialized endophytic lifestyle with tropical grasses. Interestingly, B. phytofirmans PsJN is an endophyte that successfully colonizes potato, tomato, onion roots, maize, barley and agricultural soil and has a genome of 8.2 Mbp. In this case, the large genome is not associated with pathogenicity, but harbours a broad range of physiological functions that facilitate B. phytofermans PsJN ability to colonize such a wide variety of plant species (Mitter ). Again, this demonstrates the relationship between genome size and host specialization. Over evolutionary time, as the bacteria–host plant relationship moves towards obligate mutualism, the symbiont moves towards a low-evolutionary adaptive genome (LEANOME) that is both small and devoid of mobile elements (Figure1). The minimization of genetic and metabolic redundancy is influenced by the metabolite-rich cellular environment, which lowers the selective pressure to maintain metabolic networks leading towards eventual gene loss (Moran ; Toft and Andersson, 2010).

Figure 3

Bacterial genome size associated with the stage of intracellular host adaptation. Early = facultative intracellular; advanced = obligate intracellular; extreme = obligate intracellular mutualistic (cooperative). (adapted from Toft and Andersson, 2010).

The generation of, IS-free E. coli MDS42 has been shown to generate microbial chassis with reduced ability to evolve and improved maintenance of unstable genetic constructs (Umenhoffer ). On this basis, endophytes that are at an advanced stage of mutualism with host plants may provide stable chassis for the introduction of new genetic cargo and dynamic circuits and signal transduction pathways as a result of their naturally evolved LEANOMEs; reduced genome size and low-evolutionary adaptability. Bacterial endophytes, in particular those with reduced genomes adapted to living their entire life cycles in planta, offer a wealth of role diversity and potential to understand and manipulate these mechanisms.

Synthetic approaches

In the longer term genetic and genomic analysis of selected strains will enable targeted modification of bacterial genomes in order to confer improved benefits to the crop (Straub ). Major advances are being made in synthetic biology, with whole pathways engineered into or out of bacterial genomes to modify metabolic function (Reviewed in Jarboe ). Bacterial endophytes are of particular interest as sources of traits, genes and pathways which confer benefit to the plant host and also to understand and exploit the genomic adaptations required to live within a plant without causing disease or succumbing to the plant defence systems.

Synthetic biology has been defined as ‘the engineering of biology: the synthesis of complex, biologically based (or inspired) systems, which display functions that do not exist in nature’ (EUR 21796). This engineering perspective may be applied at all levels of the hierarchy of biological structures—from individual molecules to whole cells, tissues and organisms. In essence, synthetic biology will enable the design of ‘biological systems’ in a rational and systematic way’ (Serrano, 2007). The advent of synthetic biology enables manipulation of genomes beyond the random insertion or reduced transcription of one or two genes. Not only can artificial chromosomes, multiple genes, whole pathways be introduced into new organisms at specific genomic locations, but these genes may be artificially synthesized to incorporate alternative functions or regulation, with genes also being excised completely from a genome, leaving no molecular footprint. Such approaches are accelerating rapidly in a small number of bacterial and yeast species, for example metabolic engineering of fermentation biocatalysts to produce cost effective biofuels and products (Reviewed in Jarboe ); however, their application to plants is not straightforward. Plant–microbe interactions offer a more tractable option, as the microbial partner can be targeted to produce benefits to the plant.

A major target for synthetic biology currently is to engineer BNF in nonlegumes. The identification of a host–endophyte system for crops that is capable of nitrogen fixation offers the potential of both improving crop productivity, while reducing fertilizer inputs that would result in a concomitant lowering of greenhouse gas emissions. However, despite exhaustive efforts, this mutualistic relationship, common to legumes, has not been identified in any agronomically important members of the gramineae, such as rice, wheat or maize. Recent advances in synthetic biology offer the potential to redesign and engineer the nitrogen fixation pathway into non-nitrogen-fixing bacterial hosts that live in close association with one or more of these nutritionally important food crops. Although the majority of known BNF occurs within the root nodules of legumes, this is not unique, for example Rhizobia is also capable of inducing formation of nodules with BNF capacity on the roots of Parasponia, a nonlegume in the Cannabaceae family (Cao ). Recently, Temme have targeted the DNA sequence of the nitrogen fixation (nif) gene cluster of Klebsiella oxytoca. In this landmark study, the entire nif gene cluster DNA sequence was systematically ‘refactored’ in silico (a software development term meaning that the program code has been rewritten to achieve stability while not compromising functionality, Fowler and Beck, 1999). All native regulation was removed, and the cluster placed under the control of synthetic molecular components, a toolbox of standardized parts and devices with known transcript/lational strength for each gene of the original cluster. All known and unknown regulatory sequences such as ribosomal binding sites, operators, promoters, secondary mRNA structure, and methylation pattern and pause sites in essential genes were removed by changing the codon usage (Temme ). The result was the production of a synthetic functional nif gene cluster that bore little genetic similarity to the wild-type cluster, thereby enabling orthogonal control through regulatory sensors designed on a separate plasmid. While nitrogen fixation was modest, interestingly, the synthetic BNF pathway also demonstrated nitrogen fixation in the presence of ammonia that would normally inhibit the wild-type activity. The authors have demonstrated the possibility of redesigning complex biosynthetic pathways and placing them under the regulatory control of synthetic sensors and circuits, thereby offering the potential of horizontal transfer to non-N-fixing bacterial species that live in close association with crops. Collaborative research in this area is currently being funded by both the Bill and Melinda Gates Foundation (https://www.ensa.ac.uk/home/) and the National Science Foundation (NSF) of the United States and Biotechnology and Biological Sciences Research Council (BBSRC) of the United Kingdom (http://www.bbsrc.ac.uk/funding/opportunities/2012/ideaslab-nitrogen-improving-on-nature.aspx, http://synbiology.co.uk/designing-crops-of-the-future/). The aim of these projects, respectively, is to engineer new cereal cultivars harbouring the requisite genetic factors for association with nitrogen-fixing bacteria and to engineer both an N-fixing microbe and a model grass to optimize the interaction and deliver maximum nitrogen to the plant. These combined efforts offer the potential of increasing yields of both food and lignocellulosic feedstock in sustainable agricultural systems.

While microbial synthetic biology has been undergoing rapid advancement, efforts in plant synthetic biology and the development of standardized parts and devices for uptake by plant biologists is in its infancy. In part, this may be due to the relatively lower amenability of plant systems towards targeted genome editing using tools such as transcriptional activator-like effector nucleases (TALENs) or clustered regularly interspaced short palindromic repeats (CRISPRs) and their associated proteins. Although our understanding of the double-strand break-repair mechanisms in plants is improving, the rapid advances seen in microbial systems have been confounded by the complexity of gene expression during plant development coupled with the innate recalcitrance of plant genomes to accommodate homologous recombination that would otherwise facilitate routine targeted alleic replacement (reviewed in Sun and Zhao, 2013; Puchta and Fauser, 2014). There have, however, been impressive advancements in this field (Feng ) along with the development of a genetic circuit capable of detecting the explosive trinitrotoluene (TNT) that visually reports back a decrease in the production of chlorophyll (Antunes ). The synthetic circuit was based on the work by Looger where periplasmic binding proteins (PBP), bacterial chemotactic proteins, where computationally redesigned to accept TNT as a ligand with the complex then binding to a transmembrane histidine kinase resulting in signal transduction to induce expression of β-galactosidase. In the tobacco system, this synthetic signal transduction pathway was adapted to drive expression of GUS or red chlorophyll reductase (AtRCCR), with the latter demonstrating de-greening of the plant following exposure to TNT (Antunes ).

These advances indicate the possibility of designing synthetic sensors and regulatory circuits which, when coupled together, would enable the controlled induction of bioprocessing enzymes from the endophyte upon stimulation of host signals derived during senescence. From 152 endophytic fungi and 52 endophytic bacteria, 91.7% and 64%, respectively, were found to produce xylanases (Suto ). These glycosylhydrolases are required for the deconstruction of plant biomass during biorefining processes and the production of sugar rich syrups for bioconversion into liquid fuels. It is conceivable to computationally design the PBP of bacterial endophytes to accept a specific metabolite(s) that accrue in planta during senescence to initiate transcriptional activation of endogenous or recombinant xylanases and/or other biomass processing enzymes such as ferulic acid esterases. Such synthetic plant–microbial systems may be advantageous over senescence promoter driven heterologous expression in planta (Buanafina , 2010), in terms of editing a specific location in the microbial genome, providing tightly controlled synthetic signal transduction in symbiota by a specific plant-derived metabolite, produced at a specific time, following an environmental cue. Traditional transgenic approaches can often result in obtaining only a few, or aberrant, plant phenotypes possibly arising from ‘leaky’ expression during development, nonspecific genome targeting or subcellular localization. These microbial ‘Trojan horse’ systems are suited to the sustainable agritech production of energy crops. Plant beneficial circuit characteristics (drought/salinity tolerance) may be designed and introduced into endophytes that share a strong mutualistic association with the host plant. As targeted genome editing becomes more advanced, it is possible to envision the development of synthetic sensing and effector signal transduction systems to facilitate the regulation of designer genetic circuits of dynamic metabolic pathways between crops and endophytes.

Conclusions

The opportunities for exploiting plant–microbe interactions for bioenergy crop production are numerous and diverse. The delivery of large volumes of low-cost biomass to replace existing fossil-based production must become a reality in the coming decades if we are to avoid catastrophic climate change. To achieve this at the same time as feeding the growing population is a major challenge for plant science. Land unfit for food production must be brought into use and planted with low-input perennial crops capable of producing high-biomass yields annually. Energy crops inoculated with beneficial endophytes can also be employed to phytoremediate land for future food production. Production costs must be kept to a minimum, both in economic and in energetic terms to supply low-cost sustainable biomass to the biorefinary supply chain. Low-tech applications include coating seeds with microbial biofilms as a direct method for inoculating seedlings with beneficial bacteria to aid plant growth and development. However, there are extensive possibilities for manipulation of the rhizosphere environment, by programming both the plant root exudates, and the bacterial sensing and response mechanisms. It is worth noting that the majority of the interactions in this zone are currently uncharacterized, and so it will be important to monitor soil, rhizosphere and endophyte populations. This will be a challenge as rhizosphere interactions are complex and dynamic, influenced by both addition and loss of individuals within the system (Badri ). In addition, it will be important to ensure no opportunistic pathogens have been inadvertently stimulated. Outbreaks of food poisoning from field-grown lettuce and other fresh fruit and vegetables demonstrate the gravity of this scenario (Rosenblueth and Martínez-Romero, 2006; reviewed by Nithya ). However, such modifications might in practice have major benefits to the soil, even for the subsequent crop in a rotation, similar to the potential benefit of endophytes in crop rotation to suppress nematodes (Sturz and Kimpinski, 2004). Furthermore, perennial energy crops are unlikely to form part of crop rotations where a build-up of potential human pathogens could be problematic.

Synthetic biology is already a reality in the development of novel enzymes and microbes for fermentation of biomass to fuels and other products. In the future, both plants and their beneficial symbionts will be modified to enhance biomass production for a growing population in a changing climate. Major targets for optimizing beneficial plant–microbe interactions include QS, bacterial motility, biofilm formation and the plant–microbe signalling pathways, particularly those specific to obligate endophytes. Furthermore, systematic screening of plants growing in extreme environments promises to yield novel endophytes harbouring genes and pathways conferring abiotic stress tolerance, and potentially IST, for optimization and application in energy crops destined for growth on marginal soils. The opportunities afforded by synthetic approaches, in conjunction with the minimal endophyte LEANOME, should yield a new paradigm in sustainable agriculture, with energy crops leading the way.