| Literature DB >> 25430503 |
Amir Norouzy1, Zahra Azizi, Werner M Nau.
Abstract
The macrocycle p-sulfonatocalix[4]arene (CX4) and the fluorescent dye lucigenin (LCG) form a stable host-guest complex, in which the dye fluorescence is quenched. Incubation of live V79 and CHO cells with the CX4/LCG chemosensing ensemble resulted in its spontaneous uptake. Subsequent addition of choline, acetylcholine, or protamine, which have a high affinity for CX4 and are capable of entering cells, resulted in a fluorescence switch-on response. This can be traced to the displacement of LCG from CX4 by the analytes. The results establish the principal functionality of indicator displacement assays with synthetic receptors for the detection of the uptake of bioorganic analytes by live cells.Entities:
Keywords: bioanalytical chemistry; fluorescence; host-guest complexes; macrocycles; supramolecular chemistry
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Year: 2014 PMID： 25430503 DOI： 10.1002/anie.201407808
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336