| Literature DB >> 25428067 |
Steven J Jonas1, Jackelyn A Alva2, Wade Richardson3, Sean P Sherman4, Zoran Galic5, April D Pyle6, Bruce Dunn7.
Abstract
We report the design of a chemically defined platform engineered for the culture of human pluripotent stem cells (hPSCs) that supports the long-term maintenance of self-renewing hPSC populations in a more uniform manner than standard culture systems. Microcontact printing (μCP) of alkanethiol self-assembled monolayers (SAMs) was used to spatially direct hPSC adherence. This technique not only establishes control over hPSC colony size and shape but also preserves genetic stability and provides unprecedented uniformity in the pluripotency of hPSC populations that is quantitatively assessed in the present study.Entities:
Mesh:
Substances:
Year: 2012 PMID: 25428067 PMCID: PMC4247494 DOI: 10.1016/j.msec.2012.08.035
Source DB: PubMed Journal: Mater Sci Eng C Mater Biol Appl ISSN: 0928-4931 Impact factor: 7.328