Fan Lin1, Zongming Chen. 1. From the Department of Laboratory Medicine, Geisinger Medical Center, Danville, Pennsylvania.
Abstract
CONTEXT: Immunohistochemistry has become an indispensable ancillary technique in anatomic pathology laboratories. Standardization of every step in preanalytic, analytic, and postanalytic phases is crucial to achieve reproducible and reliable immunohistochemistry test results. OBJECTIVE: To standardize immunohistochemistry tests from preanalytic, analytic, to postanalytic phases. DATA SOURCES: Literature review and Geisinger (Geisinger Medical Center, Danville, Pennsylvania) experience. CONCLUSIONS: This review article delineates some critical points in preanalytic, analytic, and postanalytic phases; reiterates some important questions, which may or may not have a consensus at this time; and updates the newly proposed guidelines on antibody validation from the College of American Pathologists Pathology and Laboratory Quality Center. Additionally, the article intends to share Geisinger's experience with (1) testing/optimizing a new antibody and troubleshooting; (2) interpreting and reporting immunohistochemistry assay results; (3) improving and implementing a total immunohistochemistry quality management program; and (4) developing best practices in immunohistochemistry.
CONTEXT: Immunohistochemistry has become an indispensable ancillary technique in anatomic pathology laboratories. Standardization of every step in preanalytic, analytic, and postanalytic phases is crucial to achieve reproducible and reliable immunohistochemistry test results. OBJECTIVE: To standardize immunohistochemistry tests from preanalytic, analytic, to postanalytic phases. DATA SOURCES: Literature review and Geisinger (Geisinger Medical Center, Danville, Pennsylvania) experience. CONCLUSIONS: This review article delineates some critical points in preanalytic, analytic, and postanalytic phases; reiterates some important questions, which may or may not have a consensus at this time; and updates the newly proposed guidelines on antibody validation from the College of American Pathologists Pathology and Laboratory Quality Center. Additionally, the article intends to share Geisinger's experience with (1) testing/optimizing a new antibody and troubleshooting; (2) interpreting and reporting immunohistochemistry assay results; (3) improving and implementing a total immunohistochemistry quality management program; and (4) developing best practices in immunohistochemistry.
Authors: B Smith; R Neff; D E Cohn; F J Backes; A A Suarez; D G Mutch; C M Rush; C J Walker; P J Goodfellow Journal: Gynecol Oncol Date: 2016-08-15 Impact factor: 5.482
Authors: Lik Hang Lee; Lindy Davis; Lourdes Ylagan; Angela R Omilian; Kristopher Attwood; Canan Firat; Jinru Shia; Philip B Paty; William G Cance Journal: J Natl Cancer Inst Date: 2022-05-09 Impact factor: 11.816