Literature DB >> 2542395

Marked and variable inhibition by chemical fixation of cytochrome oxidase and succinate dehydrogenase in single motoneurons.

G R Chalmers1, V R Edgerton.   

Abstract

The effect of tissue fixation on succinate dehydrogenase and cytochrome oxidase activity in single motoneurons of the rat was demonstrated using a computer image processing system. Inhibition of enzyme activity by chemical fixation was variable, with some motoneurons being affected more than others. It was concluded that quantification of enzymatic activity in chemically fixed tissue provides an imprecise estimate of enzyme activities found in fresh-frozen tissues.

Entities:  

Keywords:  NASA Discipline Musculoskeletal; Non-NASA Center

Mesh：

Substances：

Year:  1989        PMID： 2542395     DOI： 10.1177/37.6.2542395

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  15 in total

1.  Demonstration of 5'-nucleotidase activity in unfixed cryostat sections of rat liver using a combined light- and electron-microscope procedure.

Authors:  J Song; K S Bosch; W Tigchelaar; R J Van Den Munckhof; J P Schellens; C J Van Noorden; W M Frederiks
Journal:  Histochem J       Date:  1995-11

2.  Comparison of cell body size and oxidative enzyme activity in motoneurons between the cervical and lumbar segments in the rat spinal cord after spaceflight and recovery.

Authors:  A Ishihara; J Yamashiro; A Matsumoto; A Higashibata; N Ishioka; T Shimazu; Y Ohira
Journal:  Neurochem Res       Date:  2006-05-04       Impact factor: 3.996

3.  Cell body size and succinate dehydrogenase activity of spinal motoneurons innervating the soleus muscle in mice, rats, and cats.

Authors:  A Ishihara; Y Ohira; M Tanaka; W Nishikawa; N Ishioka; A Higashibata; R Izumi; T Shimazu; Y Ibata
Journal:  Neurochem Res       Date:  2001-12       Impact factor: 3.996

4.  Ultrastructural localization of activity of phosphatases by low temperature incubation of unfixed cryostat sections.

Authors:  J Y Song; W Tigchelaar; J P Schellens; J Van Marle; C J Van Noorden; W M Frederiks
Journal:  Histochem Cell Biol       Date:  1996-09       Impact factor: 4.304

Review 5.  Analysis of enzyme reactions in situ.

Authors:  C J Van Noorden; G N Jonges
Journal:  Histochem J       Date:  1995-02

6.  Ultrastructural localization of xanthine oxidase activity in the digestive tract of the rat.

Authors:  R J Van Den Munckhof; H Vreeling-Sindelarova; J P Schellens; C J Van Noorden; W M Frederiks
Journal:  Histochem J       Date:  1995-11

7.  Quantitative aspects of enzyme histochemistry on sections of freeze-substituted glycol methacrylate-embedded rat liver.

Authors:  W M Frederiks; K S Bosch
Journal:  Histochemistry       Date:  1993-10

Review 8.  A re-evaluation of the tissue distribution and physiology of xanthine oxidoreductase.

Authors:  A Kooij
Journal:  Histochem J       Date:  1994-12

9.  An accurate method for the quantification of cytochrome C oxidase in tissue sections.

Authors:  Miguel Melendez-Ferro; Matthew W Rice; Rosalinda C Roberts; Emma Perez-Costas
Journal:  J Neurosci Methods       Date:  2013-01-30       Impact factor: 2.390

10.  Electron microscopical study of a cytosolic enzyme in unfixed cryostat sections: demonstration of glycogen phosphorylase activity in rat liver and heart tissue.

Authors:  J P Schellens; H Vreeling-Sindelárová; R J Van den Munckhof; W M Frederiks
Journal:  Histochem J       Date:  1995-08
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.