Literature DB >> 2542354

Use of two monoclonal antibodies in an ELISA test for the detection of antibodies to bovine leukaemia virus envelope protein gp51.

D Portetelle1, M Mammerickx, A Burny.   

Abstract

A competition ELISA technique involving two monoclonal anti-gp51 antibodies has been developed for the detection of bovine leukaemia virus (BLV) antibodies. Precoated gp51 antigen-microtitre plates were obtained by incubation of plastic adsorbed monoclonal antibody with a non-purified BLV preparation. Samples to be tested were incubated in the wells of the gp51-coated plates; the presence of anti-gp51 antibodies was indicated by competition for antigen binding with an enzyme linked monoclonal antibody directed to an important epitope on gp51. This test is as sensitive as a routinely used indirect ELISA test; it is highly specific, reliable and easy to perform.

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Year:  1989        PMID： 2542354     DOI： 10.1016/0166-0934(89)90135-3

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  33 in total

1.  Suboptimal enhancer sequences are required for efficient bovine leukemia virus propagation in vivo: implications for viral latency.

Authors:  C Merezak; C Pierreux; E Adam; F Lemaigre; G G Rousseau; C Calomme; C Van Lint; D Christophe; P Kerkhofs; A Burny; R Kettmann; L Willems
Journal:  J Virol       Date:  2001-08       Impact factor: 5.103

2.  Proviral detection and serology in bovine leukemia virus-exposed normal cattle and cattle with lymphoma.

Authors:  R M Jacobs; Z Song; H Poon; J L Heeney; J A Taylor; B Jefferson; W Vernau; V E Valli
Journal:  Can J Vet Res       Date:  1992-10       Impact factor: 1.310

3.  Peripheral blood B-cell death compensates for excessive proliferation in lymphoid tissues and maintains homeostasis in bovine leukemia virus-infected sheep.

Authors:  Christophe Debacq; Nicolas Gillet; Becca Asquith; Maria Teresa Sanchez-Alcaraz; Arnaud Florins; Mathieu Boxus; Isabelle Schwartz-Cornil; Michel Bonneau; Geneviève Jean; Pierre Kerkhofs; Jack Hay; André Théwis; Richard Kettmann; Luc Willems
Journal:  J Virol       Date:  2006-10       Impact factor: 5.103

4.  CD154 costimulated ovine primary B cells, a cell culture system that supports productive infection by bovine leukemia virus.

Authors:  A Van den Broeke; Y Cleuter; T Beskorwayne; P Kerkhofs; M Szynal; C Bagnis; A Burny; P Griebel
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

5.  Purified bovine plasma blocking factor decreases Bovine leukemia virus p24 expression while increasing protein synthesis and transcriptional activity of peripheral blood mononuclear cells in short-term culture.

Authors:  Marianne J van den Heuvel; Barbara J Jefferson; Robert M Jacobs
Journal:  Can J Vet Res       Date:  2005-07       Impact factor: 1.310

6.  Cellular pathways involved in the ex vivo expression of bovine leukemia virus.

Authors:  P Kerkhofs; E Adam; L Droogmans; D Portetelle; M Mammerickx; A Burny; R Kettmann; L Willems
Journal:  J Virol       Date:  1996-04       Impact factor: 5.103

7.  Pathogenicity of molecularly cloned bovine leukemia virus.

Authors:  J Rovnak; A L Boyd; J W Casey; M A Gonda; W A Jensen; G L Cockerell
Journal:  J Virol       Date:  1993-12       Impact factor: 5.103

8.  Bovine leukemia virus-induced persistent lymphocytosis in cattle does not correlate with increased ex vivo survival of B lymphocytes.

Authors:  F Dequiedt; G H Cantor; V T Hamilton; S M Pritchard; W C Davis; P Kerkhofs; A Burny; R Kettmann; L Willems
Journal:  J Virol       Date:  1999-02       Impact factor: 5.103

9.  In vivo rescue of a silent tax-deficient bovine leukemia virus from a tumor-derived ovine B-cell line by recombination with a retrovirally transduced wild-type tax gene.

Authors:  A Van Den Broeke; C Bagnis; M Ciesiolka; Y Cleuter; H Gelderblom; P Kerkhofs; P Griebel; P Mannoni; A Burny
Journal:  J Virol       Date:  1999-02       Impact factor: 5.103

10.  Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30(T-)).

Authors:  Seong In Lim; Wooseog Jeong; Dong Seob Tark; Dong Kun Yang; Chang Hee Kweon
Journal:  J Vet Sci       Date:  2009-12       Impact factor: 1.603
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