Literature DB >> 2542350

A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus.

M Andreoni1, M Faircloth, L Vugler, W J Britt.   

Abstract

We have developed a murine monoclonal antibody reactive with a major immediate early 72,000 dalton protein of human cytomegalovirus and utilized this reagent in a rapid virus titration and microneutralization assay. Because of the early expression of this virus encoded protein, both assays could be accomplished within 16 h following virus inoculation. In addition, both assays resulted in considerable savings of reagents because the assays were carried out in 96-well microtiter plates. These assays should prove useful in the preparation and study of neutralizing antibodies directed against human cytomegalovirus.

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Year:  1989        PMID： 2542350     DOI： 10.1016/0166-0934(89)90129-8

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  104 in total

1.  A novel transferable nuclear export signal mediates CRM1-independent nucleocytoplasmic shuttling of the human cytomegalovirus transactivator protein pUL69.

Authors:  P Lischka; O Rosorius; E Trommer; T Stamminger
Journal:  EMBO J       Date:  2001-12-17       Impact factor: 11.598

2.  Functional interaction between pleiotropic transactivator pUL69 of human cytomegalovirus and the human homolog of yeast chromatin regulatory protein SPT6.

Authors:  M Winkler; T aus Dem Siepen; T Stamminger
Journal:  J Virol       Date:  2000-09       Impact factor: 5.103

3.  A continuous sequence of more than 70 amino acids is essential for antibody binding to the dominant antigenic site of glycoprotein gp58 of human cytomegalovirus.

Authors:  B Wagner; B Kropff; H Kalbacher; W Britt; V A Sundqvist; L Ostberg; M Mach
Journal:  J Virol       Date:  1992-09       Impact factor: 5.103

4.  Comparison of rapid methods of detection of cytomegalovirus in saliva with virus isolation in tissue culture.

Authors:  W P Warren; K Balcarek; R Smith; R F Pass
Journal:  J Clin Microbiol       Date:  1992-04       Impact factor: 5.948

5.  Deletion of open reading frame UL26 from the human cytomegalovirus genome results in reduced viral growth, which involves impaired stability of viral particles.

Authors:  Kerstin Lorz; Heike Hofmann; Anja Berndt; Nina Tavalai; Regina Mueller; Ursula Schlötzer-Schrehardt; Thomas Stamminger
Journal:  J Virol       Date:  2006-06       Impact factor: 5.103

6.  Sequence requirements for localization of human cytomegalovirus tegument protein pp28 to the virus assembly compartment and for assembly of infectious virus.

Authors:  Jun-Young Seo; William J Britt
Journal:  J Virol       Date:  2006-06       Impact factor: 5.103

7.  The carboxy-terminal domain of glycoprotein N of human cytomegalovirus is required for virion morphogenesis.

Authors:  Michael Mach; Karolina Osinski; Barbara Kropff; Ursula Schloetzer-Schrehardt; Magdalena Krzyzaniak; William Britt
Journal:  J Virol       Date:  2007-01-17       Impact factor: 5.103

8.  The novel anticytomegalovirus compound AIC246 (Letermovir) inhibits human cytomegalovirus replication through a specific antiviral mechanism that involves the viral terminase.

Authors:  Thomas Goldner; Guy Hewlett; Nicole Ettischer; Helga Ruebsamen-Schaeff; Holger Zimmermann; Peter Lischka
Journal:  J Virol       Date:  2011-07-13       Impact factor: 5.103

9.  Evidence for a role of the cellular ND10 protein PML in mediating intrinsic immunity against human cytomegalovirus infections.

Authors:  Nina Tavalai; Peer Papior; Sabine Rechter; Martina Leis; Thomas Stamminger
Journal:  J Virol       Date:  2006-08       Impact factor: 5.103

10.  Bicaudal D1-dependent trafficking of human cytomegalovirus tegument protein pp150 in virus-infected cells.

Authors:  Sabarish V Indran; Mary E Ballestas; William J Britt
Journal:  J Virol       Date:  2010-01-20       Impact factor: 5.103
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