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Literature DB >> 25423166

Dual channel RESOLFT nanoscopy by using fluorescent state kinetics.

Ilaria Testa¹, Elisa D'Este, Nicolai T Urban, Francisco Balzarotti, Stefan W Hell.

Abstract

We show that RESOLFT fluorescence nanoscopy, a low light level scanning superresolution technique employing reversibly switchable fluorescent proteins (rsFPs), is capable of dual-channel live-cell imaging that is virtually free of chromatic errors and temporal offsets. This is accomplished using rsEGFP and Dronpa, two rsFPs having similar spectra but different kinetics of switching and fluorescence emission. Our approach is demonstrated by imaging protein distributions and dynamics in living neurons and neuronal tissues.

Entities: Disease

Keywords: Photoswitching; lifetime; low intensities; nanoscopy

Mesh：

Substances：

Year: 2014 PMID： 25423166 DOI： 10.1021/nl503058k

Source DB: PubMed Journal: Nano Lett ISSN： 1530-6984 Impact factor: 11.189

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Cited

11 in total

1. Advancing multiscale structural mapping of the brain through fluorescence imaging and analysis across length scales.

Authors: L J Hogstrom; S M Guo; K Murugadoss; M Bathe
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2. Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom two-color stimulated emission depletion microscope.

Authors: Stephanie A Meyer; Baris N Ozbay; Mariana Potcoava; Ernesto Salcedo; Diego Restrepo; Emily A Gibson
Journal: J Biomed Opt Date: 2016-06-01 Impact factor: 3.170

3. Toward photoswitchable electronic pre-resonance stimulated Raman probes.

Authors: Dongkwan Lee; Chenxi Qian; Haomin Wang; Lei Li; Kun Miao; Jiajun Du; Daria M Shcherbakova; Vladislav V Verkhusha; Lihong V Wang; Lu Wei
Journal: J Chem Phys Date: 2021-04-07 Impact factor: 3.488

4. Encoding and decoding spatio-temporal information for super-resolution microscopy.

Authors: Luca Lanzanò; Iván Coto Hernández; Marco Castello; Enrico Gratton; Alberto Diaspro; Giuseppe Vicidomini
Journal: Nat Commun Date: 2015-04-02 Impact factor: 14.919

5. Spironaphthoxazine switchable dyes for biological imaging.

Authors: Yaoyao Xiong; Andreas Vargas Jentzsch; Johannes W M Osterrieth; Erdinc Sezgin; Igor V Sazanovich; Katharina Reglinski; Silvia Galiani; Anthony W Parker; Christian Eggeling; Harry L Anderson
Journal: Chem Sci Date: 2018-02-20 Impact factor: 9.825

Dual channel RESOLFT nanoscopy by using fluorescent state kinetics.

1. Advancing multiscale structural mapping of the brain through fluorescence imaging and analysis across length scales.

2. Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom two-color stimulated emission depletion microscope.

3. Toward photoswitchable electronic pre-resonance stimulated Raman probes.

4. Encoding and decoding spatio-temporal information for super-resolution microscopy.

5. Spironaphthoxazine switchable dyes for biological imaging.

6. Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization.

7. Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo.

8. RESOLFT nanoscopy with photoswitchable organic fluorophores.

9. 4Pi-RESOLFT nanoscopy.

10. Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity.