Literature DB >> 2542094

Characterization and expression of the rat heart sarcoplasmic reticulum Ca2+-ATPase mRNA.

A M Lompre1, D de la Bastie, K R Boheler, K Schwartz.   

Abstract

Sarcoplasmic reticulum Ca2+-ATPase cDNA clones have been isolated from an adult rat heart cDNA library and the nucleotide sequence of the Ca2+-ATPase mRNA determined. The sequence has an open reading frame of 997 codons. It is identical to a cDNA isolated from a rat stomach cDNA library and 90% isologous to the rabbit and human slow/cardiac cDNAs. Nuclease S1 mapping analysis indicates that this sequence corresponds to the main Ca2+-ATPase mRNA present in heart and in slow skeletal muscle and that it is expressed in various proportions in smooth and non-muscle tissues, together with another isoform which differs from the cardiac form in the sequence of its 3'-end.

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Year:  1989        PMID： 2542094     DOI： 10.1016/0014-5793(89)80010-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  19 in total

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