Amarel Saieg1, Kristy J Brown2, Maria T Pena3, Mary C Rose2, Diego Preciado4. 1. Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC. 2. 1] Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC [2] Department of Integrative Systems Biology, Children's National Medical Center, Washington, DC. 3. Division of Pediatric Otolaryngology-Head and Neck Surgery, Children's National Medical Center, Washington, DC. 4. 1] Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC [2] Department of Integrative Systems Biology, Children's National Medical Center, Washington, DC [3] Division of Pediatric Otolaryngology-Head and Neck Surgery, Children's National Medical Center, Washington, DC.
Abstract
BACKGROUND: Chronic rhinosinusitis (CRS) is characterized by mucous overproduction and submucosal gland hyperplasia. The global protein profile of sinonasal secretions in pediatric CRS has not been studied. We hypothesized that MUC5B, a glandular mucin, would be relatively increased in CRS secretions compared to other mucins. METHODS: Secretions were collected at Children's National Health System (Children's National) from CRS patients undergoing sinus surgery and from control patients without CRS undergoing craniofacial procedures. Proteins were extracted, digested to peptides, and analyzed by mass spectometry. Fold change significance was calculated using the QSpec algorithm. Western blot analysis was performed to validate proteomic findings. RESULTS: In total, 294 proteins were identified. Although both MUC5B and MUC5AC were identified in a majority of samples, the relative abundance of MUC5B was found to be significantly higher (P < 0.05). Western blot data validated these findings. Other proteins with the highest significant positive-fold change in CRS samples were BP1 fold-containing family A member 1, chitinase-3-like protein 1, plastin-2, serpin 10, and BP1 fold-containing family B member 1. CONCLUSION: Overall, our data demonstrate an increase of MUC5B abundance in the sinus secretions of pediatric patients with CRS.
BACKGROUND:Chronic rhinosinusitis (CRS) is characterized by mucous overproduction and submucosal gland hyperplasia. The global protein profile of sinonasal secretions in pediatric CRS has not been studied. We hypothesized that MUC5B, a glandular mucin, would be relatively increased in CRS secretions compared to other mucins. METHODS: Secretions were collected at Children's National Health System (Children's National) from CRSpatients undergoing sinus surgery and from control patients without CRS undergoing craniofacial procedures. Proteins were extracted, digested to peptides, and analyzed by mass spectometry. Fold change significance was calculated using the QSpec algorithm. Western blot analysis was performed to validate proteomic findings. RESULTS: In total, 294 proteins were identified. Although both MUC5B and MUC5AC were identified in a majority of samples, the relative abundance of MUC5B was found to be significantly higher (P < 0.05). Western blot data validated these findings. Other proteins with the highest significant positive-fold change in CRS samples were BP1 fold-containing family A member 1, chitinase-3-like protein 1, plastin-2, serpin 10, and BP1 fold-containing family B member 1. CONCLUSION: Overall, our data demonstrate an increase of MUC5B abundance in the sinus secretions of pediatric patients with CRS.
Authors: Yi Jia; Huifeng Yu; Steve M Fernandes; Yadong Wei; Anabel Gonzalez-Gil; Mary G Motari; Katarina Vajn; Whitney W Stevens; Anju T Peters; Bruce S Bochner; Robert C Kern; Robert P Schleimer; Ronald L Schnaar Journal: J Allergy Clin Immunol Date: 2015-03 Impact factor: 10.793
Authors: Kristi Biswas; Brett Wagner Mackenzie; Sharon Waldvogel-Thurlow; Martin Middleditch; Mia Jullig; Melissa Zoing; Michael W Taylor; Richard G Douglas Journal: Front Cell Infect Microbiol Date: 2017-12-06 Impact factor: 5.293
Authors: Stephanie Val; Marian Poley; Kristy Brown; Rachel Choi; Stephanie Jeong; Annie Colberg-Poley; Mary C Rose; Karuna C Panchapakesan; Joe C Devaney; Marcos Perez-Losada; Diego Preciado Journal: PLoS One Date: 2016-04-14 Impact factor: 3.240
Authors: Massimiliano Marazzato; Anna Maria Zicari; Marta Aleandri; Antonietta Lucia Conte; Catia Longhi; Luca Vitanza; Vanessa Bolognino; Carlo Zagaglia; Giovanna De Castro; Giulia Brindisi; Laura Schiavi; Valentina De Vittori; Sofia Reddel; Andrea Quagliariello; Federica Del Chierico; Lorenza Putignani; Marzia Duse; Anna Teresa Palamara; Maria Pia Conte Journal: Front Cell Infect Microbiol Date: 2020-09-02 Impact factor: 5.293