Umran Toru1, Ceylan Ayada2, Osman Genç2, Sebahat Turgut3, Günfer Turgut3, Ismet Bulut4. 1. Department of Chest Diseases, Faculty of Medicine, Dumlupınar University Kütahya 43100, Turkey. 2. Department of Physiology, Faculty of Medicine, Dumlupınar University Kütahya 43100, Turkey. 3. Department of Physiology, Faculty of Medicine, Pamukkale University Denizli 20070, Turkey. 4. Department of Allergic Disease, Süreyyapaşa Chest Diseases and Thoracic Surgery Training and Research Hospital Istanbul 34844, Turkey.
Abstract
OBJECTIVE: Genetic factors, in addition to oxidative stress factors, have been implicated in the development of chronic obstructive pulmonary disease (COPD). Multi-drug resistant-1 (MDR-1) is a gene located on chromosome 7 and the products of this gene protect lung tissue from oxidative stress. We searched the frequency of MDR-1 gene C/T polymorphism in patients with COPD and aimed to explain the association between MDR-1 gene and COPD development. METHODS: 47 patients with COPD and 64 healthy control participants were placed in this study. DNAs were extracted from blood samples and MDR-1 amplification of DNA was performed using polymerase chain reaction and enzyme digestion techniques. RESULTS: The frequencies of MDR-1 genotypes were found 17.0% for CC, 51.1% for CT and 31.9% for TT in the COPD group and 39.1% for CC, 53.1% for CT and 7.8% for TT in the control group. The distribution of MDR-1 gene C alleles were found 32.3% in COPD group and 67.7% in control group; T alleles were found 55.1% in COPD group and 44.9% in control group. There was statistically significant difference between the groups for genotype and allele frequency of MDR-1 gene (P = 0.001). CONCLUSION: TT genotype of MDR-1 gene was significantly more frequent in COPD patients. MDR-1 gene C/T polymorphism may play a role in COPD development.
OBJECTIVE: Genetic factors, in addition to oxidative stress factors, have been implicated in the development of chronic obstructive pulmonary disease (COPD). Multi-drug resistant-1 (MDR-1) is a gene located on chromosome 7 and the products of this gene protect lung tissue from oxidative stress. We searched the frequency of MDR-1 gene C/T polymorphism in patients with COPD and aimed to explain the association between MDR-1 gene and COPD development. METHODS: 47 patients with COPD and 64 healthy control participants were placed in this study. DNAs were extracted from blood samples and MDR-1 amplification of DNA was performed using polymerase chain reaction and enzyme digestion techniques. RESULTS: The frequencies of MDR-1 genotypes were found 17.0% for CC, 51.1% for CT and 31.9% for TT in the COPD group and 39.1% for CC, 53.1% for CT and 7.8% for TT in the control group. The distribution of MDR-1 gene C alleles were found 32.3% in COPD group and 67.7% in control group; T alleles were found 55.1% in COPD group and 44.9% in control group. There was statistically significant difference between the groups for genotype and allele frequency of MDR-1 gene (P = 0.001). CONCLUSION: TT genotype of MDR-1 gene was significantly more frequent in COPDpatients. MDR-1 gene C/T polymorphism may play a role in COPD development.
Authors: Margaretha van der Deen; Hendrik Marks; Brigitte W M Willemse; Dirkje S Postma; Michael Müller; Egbert F Smit; George L Scheffer; Rik J Scheper; Elisabeth G E de Vries; Wim Timens Journal: Virchows Arch Date: 2006-10-27 Impact factor: 4.064