Qintong Xu1, Zenggan Chen1, Qiong Li1, Haifei Liu1, Jian Zhang1, Wenhua Yao2, Ren Zhang2, Qingli Li3, Hongying Liu3, Feng Zhang1, William C Lineaweaver4. 1. Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University Shanghai, China. 2. Center of Analysis and Measurement, Fudan University Shanghai, China. 3. Key Laboratory of Polar Materials and Devices, East China Normal University Shanghai, China. 4. Joseph M. Still Burn and Reconstructive Center, Central Mississippi Medical Center 1850 Chadwick Drive, Suite 1427 North Tower, 4 West Jackson, MS 39204.
Abstract
OBJECTIVE: The purpose of this study was to explore the possibility for analyzing and differentiating between motor and sensory functions of peripheral nerve axons using spectral technology. METHODS: 10 μm slide section of S1 anterior and posterior rabbit spinal nerve roots were made and then stained with Karnovsky-Roots method for molecular hyperspectral imaging microscopy analysis. In addition, Raman spectra data of nerve axons on each slide was collected after Karnovsky-Roots staining for 30 minutes. RESULTS: Motor axons were differentiated from sensory axons in a nerve axon section hyperspectral image via Spectral angle mapper algorithm. Raman scatterings could be detected near 2110 cm(-1), and 2155 cm(-1) in motor axons after Karnvosky-Roots staining. The value of I2100/I1440 in motor axons are significantly different (P0.001) than in sensory axons after staining for 30 minutes. CONCLUSIONS: Motor and sensory nerve axons can be differentiated from their counterparts in 30 minutes by using Raman micro-spectroscopy analysis assisted with Karnovsky-Roots staining.
OBJECTIVE: The purpose of this study was to explore the possibility for analyzing and differentiating between motor and sensory functions of peripheral nerve axons using spectral technology. METHODS: 10 μm slide section of S1 anterior and posterior rabbit spinal nerve roots were made and then stained with Karnovsky-Roots method for molecular hyperspectral imaging microscopy analysis. In addition, Raman spectra data of nerve axons on each slide was collected after Karnovsky-Roots staining for 30 minutes. RESULTS: Motor axons were differentiated from sensory axons in a nerve axon section hyperspectral image via Spectral angle mapper algorithm. Raman scatterings could be detected near 2110 cm(-1), and 2155 cm(-1) in motor axons after Karnvosky-Roots staining. The value of I2100/I1440 in motor axons are significantly different (P0.001) than in sensory axons after staining for 30 minutes. CONCLUSIONS: Motor and sensory nerve axons can be differentiated from their counterparts in 30 minutes by using Raman micro-spectroscopy analysis assisted with Karnovsky-Roots staining.
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