Frieder Schaumburg1, Abraham S Alabi2, Harry Kaba2, Bertrand Lell2, Karsten Becker3, Martin P Grobusch4, Peter G Kremsner2, Alexander Mellmann5. 1. Institute of Medical Microbiology, University Hospital Münster, 48149 Münster, Germany Centre de Recherches Médicales de Lambaréné (CERMEL), PB 118 Lambaréné, Gabon frieder.schaumburg@ukmuenster.de. 2. Centre de Recherches Médicales de Lambaréné (CERMEL), PB 118 Lambaréné, Gabon Institut für Tropenmedizin, Eberhard Karls Universität Tübingen, Deutsches Zentrum für Infektionsforschung, 72074 Tübingen, Deutschland. 3. Institute of Medical Microbiology, University Hospital Münster, 48149 Münster, Germany. 4. Centre de Recherches Médicales de Lambaréné (CERMEL), PB 118 Lambaréné, Gabon Institut für Tropenmedizin, Eberhard Karls Universität Tübingen, Deutsches Zentrum für Infektionsforschung, 72074 Tübingen, Deutschland Center of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Division of Internal Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. 5. Institute of Hygiene, University Hospital Münster, 48149 Münster, Germany.
Abstract
BACKGROUND: Shigella spp. dysentery is widespread in developing countries; the incidence is particularly high in children between 1-2 years of age. In sub-Saharan Africa, there is a paucity of epidemiological data on Shigella spp., with possible negative consequences for recognition and correct treatment choice for this life-threatening bacterial infection. We therefore characterized Shigella spp. isolates from Gabon. METHODS: The antimicrobial resistance, virulence factors, genotypes and mobile genetic elements of Shigella isolates (29 S. flexneri; 5 S. boydii; 3 S. sonnei) from a retrospective strain collection were analyzed. RESULTS: High resistance rates were found for gentamicin and tetracycline (100%, 37/37), cotrimoxazole (92%, 34/37) and ampicillin (84%, 31/37). All isolate harbored ial and ipaH; no isolate produced Shiga toxins (stx1/2); enterotoxins (set1A/B) were only found in S. flexneri (n=19). Multilocus sequence types (MLST) clustered with global clones. A high prevalence of atypical class 1 integrons harboring blaOXA30 and aadA1 were detected in S. flexneri, while all S. sonnei carried class 2 integrons. CONCLUSIONS: There is a strong link of Gabonese Shigella spp. isolates with pandemic lineages as they cluster with major global clones and frequently carry atypical class 1 integrons which are frequently reported in Shigella spp. from Asia.
BACKGROUND: Shigella spp. dysentery is widespread in developing countries; the incidence is particularly high in children between 1-2 years of age. In sub-Saharan Africa, there is a paucity of epidemiological data on Shigella spp., with possible negative consequences for recognition and correct treatment choice for this life-threatening bacterial infection. We therefore characterized Shigella spp. isolates from Gabon. METHODS: The antimicrobial resistance, virulence factors, genotypes and mobile genetic elements of Shigella isolates (29 S. flexneri; 5S. boydii; 3 S. sonnei) from a retrospective strain collection were analyzed. RESULTS: High resistance rates were found for gentamicin and tetracycline (100%, 37/37), cotrimoxazole (92%, 34/37) and ampicillin (84%, 31/37). All isolate harbored ial and ipaH; no isolate produced Shiga toxins (stx1/2); enterotoxins (set1A/B) were only found in S. flexneri (n=19). Multilocus sequence types (MLST) clustered with global clones. A high prevalence of atypical class 1 integrons harboring blaOXA30 and aadA1 were detected in S. flexneri, while all S. sonnei carried class 2 integrons. CONCLUSIONS: There is a strong link of Gabonese Shigella spp. isolates with pandemic lineages as they cluster with major global clones and frequently carry atypical class 1 integrons which are frequently reported in Shigella spp. from Asia.
Authors: Christine J D Guglielmino; Asha Kakkanat; Brian M Forde; Sally Rubenach; Lea Merone; Russell Stafford; Rikki M A Graham; Scott A Beatson; Amy V Jennison Journal: Eur J Clin Microbiol Infect Dis Date: 2020-09-04 Impact factor: 3.267