Organotropism of latent herpes simplex virus type 1 is correlated to the presence of a 1.5 kb RNA transcript mapped within the BamHI DNA fragment B (0.738 to 0.809 map units).

The transcriptional activity of the DNA sequences within the genome of herpes simplex virus type 1 (HSV-1) at the coordinates 0.760 to 0.762 and their influence in the process of viral latency were investigated. Seven avirulent HSV-1 strains (HFEM, 1752/2, 1752/3, 1752/11, 1469, 1475, 1618), two virulent wild-type HSV-1 strains (F and 17) and three virulent intratypic HSV-1 recombinant viruses (R19, R26, RM1C1) were screened. The virulent HSV-1 strains colonize the ganglia but the avirulent virus strains are only able to persist in the spleen of infected animals (tree shrews). A 1.5 kb RNA transcript was detectable in all virus strains recovered from the ganglia. This RNA transcript hybridised to the HSV-1 DNA sequences at the genome coordinates 0.760 to 0.762 (BssHII DNA fragment F, part of the BamHI DNA fragment B of HSV-1, 0.738 to 0.809 map units (m.u.]. In contrast it was found that the 1.5 kb RNA transcript was missing or its size was changed in cells infected with those HSV-1 strains which were recovered from the spleens of latently infected animals. The state of viral latency of three defined deletion variants of HSV-1 strain 17 (1704, 1705, and 1706) whose genome harbors deletions (2.2 to 5.3 kb) comprising the DNA sequences of the particular region (0.760 to 0.762 m.u.) was investigated. These studies revealed that all three deletion variants could only be recovered from the spleens of latently infected tree shrews.