A viable HSV-1 mutant deleted in two nonessential major glycoproteins.

A HSV-1 recombinant lacking the two major glycoproteins gC and gE was isolated from cells co-infected with mutants negative for only one of these glycoproteins. The deletions of the appropriate genes were shown to be the same as on the respective parental genomes. In cell culture, the gC/gE minus recombinant virus replicated to titers similar to those obtained with the gC minus parental strain.