Literature DB >> 25415185

Collagen labelling with an azide-proline chemical reporter in live cells.

Beatrice Amgarten1, Rakesh Rajan, Nuria Martínez-Sáez, Bruno L Oliveira, Inês S Albuquerque, Roger A Brooks, David G Reid, Melinda J Duer, Gonçalo J L Bernardes.   

Abstract

We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide-alkyne cycloaddition reaction.

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Year:  2015        PMID: 25415185     DOI: 10.1039/c4cc07974d

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN: 1359-7345            Impact factor:   6.222


  1 in total

1.  Importance of dipole moments and ambient polarity for the conformation of Xaa-Pro moieties - a combined experimental and theoretical study.

Authors:  Christiane Siebler; Boris Maryasin; Michael Kuemin; Roman S Erdmann; Carla Rigling; Claudio Grünenfelder; Christian Ochsenfeld; Helma Wennemers
Journal:  Chem Sci       Date:  2015-08-12       Impact factor: 9.825

  1 in total

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