OBJECTIVE: To investigate a role of Vitamin D in the pathogenesis of preeclampsia (PE), and to discern any potential benefits of Vitamin D supplementation on hypertension in the RUPP rat model of PE. STUDY DESIGN: Blood and placentas from normal pregnancies (NP) and PE were collected following elective cesarean delivery without evidence of infection. Circulating Vitamin D was extracted by HPLC and measured via mass spectrometry. Media for placenta explants was supplemented with Vitamin D and exposed to hypoxic (1% O2) or normoxic (6% O2) conditions for 24 hours. ELISAs were performed on media and normalized to total protein to determine cytokine secretion. RUPP rats were supplemented with vitamin D by oral gavage, and blood pressure (MAP) and pup weights were measured in NP and RUPP rats with or without Vitamin D supplementation. Flow cytometry was used to evaluate CD4+ Tcells in control RUPP rats and RUPP rats treated with Vitamin D. RESULTS: Inflammatory cytokine secretion was higher (p<0.05) while the anti-inflammatory cytokine, IL-10, was significantly lower in the media of PE placentas compared to NP (p=0.005). Vitamin D supplementation decreased hypoxia stimulated pro-inflammatory cytokine secretion (p=0.003) in the media of PE placentas. Vitamin D decreased MAP and circulating CD4+ T cells in the RUPP rat model of PE (p<0.05). CONCLUSION: Vitamin D supplementation may be useful in the treatment or prevention of hypertensive disorders in pregnancy.
OBJECTIVE: To investigate a role of Vitamin D in the pathogenesis of preeclampsia (PE), and to discern any potential benefits of Vitamin D supplementation on hypertension in the RUPPrat model of PE. STUDY DESIGN: Blood and placentas from normal pregnancies (NP) and PE were collected following elective cesarean delivery without evidence of infection. Circulating Vitamin D was extracted by HPLC and measured via mass spectrometry. Media for placenta explants was supplemented with Vitamin D and exposed to hypoxic (1% O2) or normoxic (6% O2) conditions for 24 hours. ELISAs were performed on media and normalized to total protein to determine cytokine secretion. RUPPrats were supplemented with vitamin D by oral gavage, and blood pressure (MAP) and pup weights were measured in NP and RUPPrats with or without Vitamin D supplementation. Flow cytometry was used to evaluate CD4+ Tcells in control RUPPrats and RUPPrats treated with Vitamin D. RESULTS: Inflammatory cytokine secretion was higher (p<0.05) while the anti-inflammatory cytokine, IL-10, was significantly lower in the media of PE placentas compared to NP (p=0.005). Vitamin D supplementation decreased hypoxia stimulated pro-inflammatory cytokine secretion (p=0.003) in the media of PE placentas. Vitamin D decreased MAP and circulating CD4+ T cells in the RUPPrat model of PE (p<0.05). CONCLUSION:Vitamin D supplementation may be useful in the treatment or prevention of hypertensive disorders in pregnancy.
Entities:
Keywords:
Cytokines; Hypertension; Preeclampsia; Vitamin D
Authors: B T Alexander; S E Kassab; M T Miller; S R Abram; J F Reckelhoff; W A Bennett; J P Granger Journal: Hypertension Date: 2001-04 Impact factor: 10.190
Authors: Bhavisha A Bakrania; Frank T Spradley; Heather A Drummond; Babbette LaMarca; Michael J Ryan; Joey P Granger Journal: Compr Physiol Date: 2020-12-09 Impact factor: 9.090
Authors: Jessica L Faulkner; Denise C Cornelius; Lorena M Amaral; Ashlyn C Harmon; Mark W Cunningham; Marie M Darby; Tarek Ibrahim; D'Andrea S Thomas; Florian Herse; Gerd Wallukat; Ralf Dechend; Babbette LaMarca Journal: Am J Physiol Regul Integr Comp Physiol Date: 2015-12-16 Impact factor: 3.619
Authors: Ashlyn C Harmon; Denise C Cornelius; Lorena M Amaral; Jessica L Faulkner; Mark W Cunningham; Kedra Wallace; Babbette LaMarca Journal: Clin Sci (Lond) Date: 2016-03 Impact factor: 6.124