| Literature DB >> 25413837 |
Haruko Miyazaki1, Fumitaka Oyama2, Ritsuko Inoue3, Toshihiko Aosaki3, Takaya Abe4, Hiroshi Kiyonari4, Yoshihiro Kino1, Masaru Kurosawa1, Jun Shimizu5, Ikuo Ogiwara6, Kazuhiro Yamakawa6, Yoshinori Koshimizu7, Fumino Fujiyama8, Takeshi Kaneko7, Hideaki Shimizu9, Katsuhiro Nagatomo10, Katsuya Yamada10, Tomomi Shimogori11, Nobutaka Hattori12, Masami Miura3, Nobuyuki Nukina1.
Abstract
Voltage-gated Na(+) channel β-subunits are multifunctional molecules that modulate Na(+) channel activity and regulate cell adhesion, migration and neurite outgrowth. β-subunits including β4 are known to be highly concentrated in the nodes of Ranvier and axon initial segments in myelinated axons. Here we show diffuse β4 localization in striatal projection fibres using transgenic mice that express fluorescent protein in those fibres. These axons are unmyelinated, forming large, inhibitory fibre bundles. Furthermore, we report β4 dimer expression in the mouse brain, with high levels of β4 dimers in the striatal projection fascicles, suggesting a specific role of β4 in those fibres. Scn4b-deficient mice show a resurgent Na(+) current reduction, decreased repetitive firing frequency in medium spiny neurons and increased failure rates of inhibitory postsynaptic currents evoked with repetitive stimulation, indicating an in vivo channel regulatory role of β4 in the striatum.Entities:
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Year: 2014 PMID： 25413837 DOI： 10.1038/ncomms6525
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919