BACKGROUND: Novel biomarkers of exposure and early adverse effects are needed for comparative studies of combustible and non-combustible tobacco products for regulatory authority evaluation. Metabolic biomarkers reflect both gene and environmental effects. RESULTS: CE-MS has been applied to human urine samples from non-smokers and smokers of cigarettes at two tar levels. Validated chemometric models were able to separate smokers from non-smokers, with discrimination mainly based on the presence of nicotine metabolites. With these removed, it still proved possible to discriminate smokers from non-smokers with models now based on endogenous metabolites. The biochemical relevance of these biomarkers is discussed. CONCLUSION: This proof-of-principle metabonomics study illustrates the potential of CE-MS to discover novel biomarkers in urine from tobacco users.
BACKGROUND: Novel biomarkers of exposure and early adverse effects are needed for comparative studies of combustible and non-combustible tobacco products for regulatory authority evaluation. Metabolic biomarkers reflect both gene and environmental effects. RESULTS: CE-MS has been applied to human urine samples from non-smokers and smokers of cigarettes at two tar levels. Validated chemometric models were able to separate smokers from non-smokers, with discrimination mainly based on the presence of nicotine metabolites. With these removed, it still proved possible to discriminate smokers from non-smokers with models now based on endogenous metabolites. The biochemical relevance of these biomarkers is discussed. CONCLUSION: This proof-of-principle metabonomics study illustrates the potential of CE-MS to discover novel biomarkers in urine from tobacco users.
Authors: Peter G Shields; Micah Berman; Theodore M Brasky; Jo L Freudenheim; Ewy Mathe; Joseph P McElroy; Min-Ae Song; Mark D Wewers Journal: Cancer Epidemiol Biomarkers Prev Date: 2017-06-22 Impact factor: 4.254
Authors: Huiwei Zhu; Abu S Abdullah; Jingyi He; Jianxiong Xi; Yimeng Mao; Yitian Feng; Qianyi Xiao; Pinpin Zheng Journal: Int J Environ Res Public Health Date: 2021-01-15 Impact factor: 3.390