Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 CRP acts as a transcriptional repressor of the YPO1635-phoPQ-YPO1632 operon in Yersinia pestis.

Literature DB >> 25413606

CRP acts as a transcriptional repressor of the YPO1635-phoPQ-YPO1632 operon in Yersinia pestis.

Yiquan Zhang¹, Fengjun Sun, Huiying Yang, Lei Liu, Bin Ni, Xinxiang Huang, Ruifu Yang, Dongsheng Zhou.

Abstract

Yersinia pestis is the causative agent of plague. Both cyclic AMP receptor protein (CRP) and PhoP are involved in regulating virulence-related genes in Y. pestis. The phoPQ loci are transcribed as two distinct operons, YPO1635-phoPQ-YPO1632 and phoPQ-YPO1632. In the present work, the regulation of the first operon by CRP was investigated using primer extension, LacZ fusion, electrophoretic mobility shift assay, and DNase I footprinting assays. The results showed that CRP bound to a DNA region overlapping core promoter -10 element and transcription start of YPO1635 to repress the expression of YPO1635-phoPQ-YPO1632. Taken together with our previous results, complex regulatory interactions of CRP-AMP and PhoP/PhoQ were proposed in Y. pestis, which would contribute to tightly controlled expression of virulence-related genes.

Entities: Chemical Species

Mesh：

Substances：

Year: 2014 PMID： 25413606 DOI： 10.1007/s00284-014-0736-z

Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.188

20 in total

Review 1. The pleiotropic two-component regulatory system PhoP-PhoQ.

Authors: E A Groisman
Journal: J Bacteriol Date: 2001-03 Impact factor: 3.490

2. A plasminogen-activating protease specifically controls the development of primary pneumonic plague.

Authors: Wyndham W Lathem; Paul A Price; Virginia L Miller; William E Goldman
Journal: Science Date: 2007-01-26 Impact factor: 47.728

3. Role of the Yersinia pestis plasminogen activator in the incidence of distinct septicemic and bubonic forms of flea-borne plague.

Authors: Florent Sebbane; Clayton O Jarrett; Donald Gardner; Daniel Long; B Joseph Hinnebusch
Journal: Proc Natl Acad Sci U S A Date: 2006-03-27 Impact factor: 11.205

4. Identification of the CRP regulon using in vitro and in vivo transcriptional profiling.

Authors: Dongling Zheng; Chrystala Constantinidou; Jon L Hobman; Stephen D Minchin
Journal: Nucleic Acids Res Date: 2004-11-01 Impact factor: 16.971

5. Cyclic AMP and its receptor protein negatively regulate the coordinate expression of cholera toxin and toxin-coregulated pilus in Vibrio cholerae.

Authors: K Skorupski; R K Taylor
Journal: Proc Natl Acad Sci U S A Date: 1997-01-07 Impact factor: 11.205

6. Regulation of the Vibrio vulnificus hupA gene by temperature alteration and cyclic AMP receptor protein and evaluation of its role in virulence.

Authors: Man Hwan Oh; Sung Min Lee; Dong Hwan Lee; Sang Ho Choi
Journal: Infect Immun Date: 2009-01-12 Impact factor: 3.441

7. The cyclic AMP receptor protein, CRP, is required for both virulence and expression of the minimal CRP regulon in Yersinia pestis biovar microtus.

Authors: Lingjun Zhan; Yanping Han; Lei Yang; Jing Geng; Yingli Li; He Gao; Zhaobiao Guo; Wei Fan; Gang Li; Lianfeng Zhang; Chuan Qin; Dongsheng Zhou; Ruifu Yang
Journal: Infect Immun Date: 2008-08-18 Impact factor: 3.441

8. Essential role for cyclic AMP and its receptor protein in Yersinia enterocolitica virulence.

Authors: Shane Petersen; Glenn M Young
Journal: Infect Immun Date: 2002-07 Impact factor: 3.441

9. A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor.

Authors: Lisa Rickman; Colin Scott; Debbie M Hunt; Thomas Hutchinson; M Carmen Menéndez; Rachael Whalan; Jason Hinds; M Joseph Colston; Jeffrey Green; Roger S Buxton
Journal: Mol Microbiol Date: 2005-06 Impact factor: 3.501