| Literature DB >> 25412204 |
Jens Laurids Sørensen1, Teis Esben Sondergaard, Lorenzo Covarelli, Patricia Romans Fuertes, Frederik Teilfeldt Hansen, Rasmus John Normand Frandsen, Wagma Saei, Mie Bech Lukassen, Reinhard Wimmer, Kristian Fog Nielsen, Donald Max Gardiner, Henriette Giese.
Abstract
The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS.Entities:
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Year: 2014 PMID： 25412204 DOI： 10.1021/np500436r
Source DB: PubMed Journal: J Nat Prod ISSN： 0163-3864 Impact factor: 4.050