Holly D Cox1, Cole M Hughes, Daniel Eichner. 1. Sports Medicine Research & Testing Laboratory, 560 Arapeen Drive, Suite 150A, Salt Lake City, UT 84108, USA.
Abstract
BACKGROUND: Dried blood spot sample collection could improve detection of the misuse of IGF-1, its analogs and growth hormone. An LC-MS/MS method was developed to measure two IGF-1 peptides and one analog peptide after trypsin digestion. In addition to standard method validation parameters, the effect of hematocrit on cysteine alkylation, trypsin digestion and the selection of internal standard were evaluated. RESULTS: Quantification of IGF-1 peptides was possible with an LLOQ of 25 ng/ml and imprecision of less than 15%. CONCLUSION: While the effects of hematocrit must be evaluated empirically for each method, dried blood spots are a suitable matrix for the measurement of IGF-1 and its analogs by MS.
BACKGROUND: Dried blood spot sample collection could improve detection of the misuse of IGF-1, its analogs and growth hormone. An LC-MS/MS method was developed to measure two IGF-1 peptides and one analog peptide after trypsin digestion. In addition to standard method validation parameters, the effect of hematocrit on cysteine alkylation, trypsin digestion and the selection of internal standard were evaluated. RESULTS: Quantification of IGF-1 peptides was possible with an LLOQ of 25 ng/ml and imprecision of less than 15%. CONCLUSION: While the effects of hematocrit must be evaluated empirically for each method, dried blood spots are a suitable matrix for the measurement of IGF-1 and its analogs by MS.