Literature DB >> 25410290

Determination of Functional Activity of Human iPSC-Derived Hepatocytes by Measurement of CYP Metabolism.

Pooja Chaudhari1, Neha Prasad1, Lipeng Tian1, Yoon-Young Jang2.   

Abstract

The advent of induced pluripotent stem cell (iPSC) technology has enabled the modeling of an array of specific human disease phenotypes, aiding in the increasingly important and indispensable understanding of disease progression and pathogenesis. Pluripotent stem cell-derived hepatocytes present a new avenue for drug screening and personalized drug testing toward precision medicine. CYP450 microsomal enzymes play a critical role in drug metabolism. Hence, CYP activity measurement of iPSC-derived hepatocytes is a vital prerequisite, to ensure metabolic functionality before proceeding to drug testing. Herein, we describe the protocol for measurement of different CYP450 enzyme activities in human iPSC-derived hepatocytes.

Entities:  

Keywords:  CYP450; Drug metabolism; Hepatic differentiation; Liver function; iPSCs

Mesh：

Substances：

Year:  2016        PMID： 25410290     DOI： 10.1007/7651_2014_145

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

Review 1.  Expression kinetics of hepatic progenitor markers in cellular models of human liver development recapitulating hepatocyte and biliary cell fate commitment.

Authors:  Pooja Chaudhari; Lipeng Tian; Abhijeet Deshmukh; Yoon-Young Jang
Journal:  Exp Biol Med (Maywood)       Date:  2016-07-06

Review 2.  Current Management of Alcoholic Hepatitis and Future Therapies.

Authors:  Behnam Saberi; Alia S Dadabhai; Yoon-Young Jang; Ahmet Gurakar; Esteban Mezey
Journal:  J Clin Transl Hepatol       Date:  2016-06-15

3.  A comparison of hepato-cellular in vitro platforms to study CYP3A4 induction.

Authors:  Beyza Bulutoglu; Camilo Rey-Bedón; Safak Mert; Lipeng Tian; Yoon-Young Jang; Martin L Yarmush; O Berk Usta
Journal:  PLoS One       Date:  2020-02-27       Impact factor: 3.240

4.  Alcohol Increases Liver Progenitor Populations and Induces Disease Phenotypes in Human IPSC-Derived Mature Stage Hepatic Cells.

Authors:  Lipeng Tian; Abhijeet Deshmukh; Neha Prasad; Yoon-Young Jang
Journal:  Int J Biol Sci       Date:  2016-07-18       Impact factor: 6.580

5.  An in vitro model of hepatic steatosis using lipid loaded induced pluripotent stem cell derived hepatocyte like cells.

Authors:  Hiraganahalli Bhaskar Deepak; Nellikalaya Shreekrishna; Zaheerbasha Sameermahmood; Niranjan Naranapur Anand; Raghotham Hulgi; Juluri Suresh; Sonal Khare; Saravanakumar Dhakshinamoorthy
Journal:  J Biol Methods       Date:  2020-07-22
  5 in total

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